Role of Cytokines in Patients with Arthritis and Low Back Pain Brucellosis
Research center of Immunology and Immunology
Nayyereh
Saadati
Associate Professor of Immunology Department of Internal Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Reza
Farid Hoseini
Professor of Immunology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Jalil
Tvakol Afshari
Associate Professor of Immunology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Amir Reza
Khaligi
Infectious Disease Specialist, Mashhad University of Medical Sciences, Mashhad, Iran
author
Bahram
Naghib zadeh
Intern, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction Cytokines are secreted as the molecular products of different cells in particular T–Lymphocyte which contribute in the processes of immune responses. Therefore, the study may provide valuable information regarding the function of cell–mediated immunity ( CMI ) in this disease. .Materials and Methods This study was performed in the medical centers of Mashhad University of Medical Science. 40 Patients with brucellosis and 40 normal samples took part in this investigation. Diagnosis of the disease was based on case history clinical examination and serological tests. Results The levels of TNF-α, IL-2, IFN–γ, IL-4, and IL–10 in serum were measured using ELISA method. The levels of the random variables IL-2 and IL-10 were tested by Kolmogrov Smirnov test and shown to be normal (p<0.05). Other cytokines including IL-4, IFN–γ, and TNF-α of patients serum were not normal. The results indicated that the levels of IL-10 in the sera of patients had increased (p<0.001). IFN–γ level showed it has increased in patients (p<0.05). The level of IL–2 and IL–4 and TNF-α in the sera of patients did not show any appreciable difference with those of normal controls (p>0.1). Kruskal-Wallis test showed a significant difference in IFN–γ in the two groups. Conclusion Our results would suggest a definite role of IFN–γ, and IL-10 in sera of patients with arthritis and low bake pain brucellosis.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
133
137
https://mjms.mums.ac.ir/article_5375_1738ff2af0476f16ec6e3d1ad3054d60.pdf
dx.doi.org/10.22038/mjms.2010.5375
Evaluation of Mulecular Epidemiology of Cutaneous Leishmaniasis In Sabzevar
Masoud
Mohajery
Associate Professor, Parasitology & Medical Mycology Department, Mashhad University of Medical Sciences, Mashhad, Iran
author
Seyad Aliakbar
Shamsian
Assistant Professor, Iranian Academic Center of Education, Culture& Research, Mashhad Branch
author
Abdolrahim
rezaee
Assistant Professor, Virology Mashhad University of Medical Sciences, Mashhad, Iran
author
Kazem
Hasan Poor
Specialist In Pediatric Medicine
author
Mohammad taghi
Shakeri
Associate Professor of Social Medicine,University of Medical Sciences, Mashhad, Iran
author
Gholamreza
Farnoosh
Master Of Science In Parasitology
author
Farhad
Fathi Moghadam
Research Instructor, Iranian Academic Center of Education, Culture& Research, Mashhad Branch
author
text
article
2010
per
Introduction Cutaneous leishmaniasis is a parasitic infection With an important health problem in many parts of Iran such as Sabzevar, in Khorasan Razavi province. Epidemiological and clinical findings aren’t sufficient for identification of parasites. Because the host sources are different an accurate identification and diagnosis is necessary before treatment. DNA of every parasite such as every organism is specific. This facilitates extensive use of DNA for diagnostic and identification of parasite species. Molecular methods such as PCR seem to be very useful for this reason. We decided to identify different species of leishmania parasites causing Cutaneous leishmaniasis by PCR in Sabzevar Materials and Methods A Total of 86 patients, whom diseases were confirmed by direct smear, were recruited and samples were isolated and cultured in NNN medium, followed by sub-cultured in RPMI-1640. Then DNA was extracted using four DNA extraction methods. Extracted kinetoplastic DNA was amplified by PCR method using two specific primers. Electrophoresis patterns from each isolate were compared with reference strains of L.major, L.tropica and the marker Results The related bands to amplified products were detected on agarose gel in all samples expected of DNA extracted by boiling method. The results of kDNA gene templets in Electrophoresis gel indicated the leishmania parasite species, causing Cutaneous leishmaniasis, in Sabzevar as 32 samples L.tropica and 54 samples L.major. Conclusion L.tropica and L.major both are Etiologic agents ofCutaneous leishmaniasis in Sabzevar and PCR technique is a suitable tool for the leishmania species characterization in epidemiological studies. The phenol-chloroform based methods are as valuable as DNeasy mini kit (QIAGEN) but more cost effective than kit.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
138
144
https://mjms.mums.ac.ir/article_5376_03d9e1b6245b5f906f10ff25d6c898c4.pdf
dx.doi.org/10.22038/mjms.2010.5376
Correlation between the Result of Leishmanin Skin Test And
Clinical Forms of Cutaneous Leishmaniasis and Species of Parasite
Research Center for Skin Diseases & Cutaneous Leishmaniasis
Elham
Shirangi
General Practitioner
author
Farzaneh
Maaleki
Assistant Professor of Dermatology, Azad University of Medical Sciences, Mashhad, Iran
author
Monavar
Afzal-aghae
Community Medicine specialist, Mashhad University of Medical Sciences, Mashhad, Iran
author
Saba
Fata
General Practitioner
author
Maryam
Ghaffarian
General Practitioner
author
Mojtaba
Meshkat
Biometry Expert
author
Abdolmajid
Fata
Professor of Mycology, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction Cutaneous Leishmaniasis (CL) is a common parasitic skin disease which is endemic in many parts of Iran, including Khorasan. Leishmanin Skin Test (LST) is used for clinical diagnosis of previous infection. The aim of this study was to determine the correlation between the result of LST with the clinical form of CL lesion and species of causative agent in patients referred to the Parasitology laboratory, Imam Reza hospital during 2007-2008. Materials and Methods This study was undertaken over a 12 month period. Following confirmation of diagnosis of CL in 50 patients who obtained positive result by direct smear test, their demographic, clinical and laboratory information were recorded. LST was performed for each patient. PCR (polymerase chain reaction) was used to determine the species of parasite. The result of LST read after 24, 48 and 72 hours.The collected data were statistically analyzed using SPSS. Results Among 30 persons with Anthroponotic Cutaneous Leishmaniasis (ACL), 60% had dry and 33% had infected moist lesions. Among 20 persons with Zoonotic Cutaneous Leishmaniasis (ZCL), dry and infected moist ulcers were observed in 30% and 65% respectively. The result of Positive LSTwas increased 48 and 72 hrs. After injection. Conclusion There was a significant relationship between the clinical form of CL lesion and causative agent as the result of the LST; but no significant relationship was observed between causative agent & clinical form.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
145
151
https://mjms.mums.ac.ir/article_5378_eff8061d6ad71af13db1fa8d9deacade.pdf
dx.doi.org/10.22038/mjms.2010.5378
The Effect of Hearing Loss on Acoustic Parameters of Voice in Children
Omid
Mohammadi
Master of Science in Speech Therapy, Dept. Of Speech Therapy, Faculty of Rehabilitation, Semnan University of Medical Sciences and Health Services, Semnan, Iran
author
Ali
Sadolahi
Master of Science in Speech Therapy, Dept. Of Speech Therapy, Faculty of Rehabilitation, Semnan University of Medical Sciences and Health Services, Semnan, Iran.
author
Raheb
Ghorbani
Associate Professor in Biostatics, Dept. Of Social Medicine, Faculty of Medicine, Semnan University of Medical Sciences and Health Services, Semnan, Iran.
author
text
article
2010
per
Introduction By means of normal hearing system and appropriate auditory feedback, we can control acoustic parameters of voice such as phonation, quality of voice, pitch and intensity. Materials and Methods The aim of this cross-sectional study was investigation and comparison of some acoustic parameters of voice including: mean of intensity range, frequency range, shimmer, and harmonic to noise ratio between 2 groups of children (17 hearing impaired and 17 normal children). These groups were matched with respect to age and sex. The mean of each parameters were calculated using collection of speech studio software and electrolaryngograph apparatus and were compared between two groups of children. Results The mean of intensity range between two groups was not statistically different (P=0.462). The mean of frequency range between two groups was not statistically different either (P=0.277). The mean of Shimmer in children with hearing loss was higher than normal children (P=0.010). The mean of Harmonic to noise ratio (HNR) in children with hearing loss was lower than normal children (P=0.001). Conclusion It can be concluded that most susceptible acoustic parameters of voice for improving by hearing aids and speech therapy services are frequency and intensity range. The higher mean of shimmer and lower mean of harmonic to noise ratio (HNR) in hearing impaired children in comparison with normal group is caused by excessive pressure that these children are bearing on the larynx and related structures.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
152
157
https://mjms.mums.ac.ir/article_5379_3fd78a0540abcf33ef54159e2ec87d9e.pdf
dx.doi.org/10.22038/mjms.2010.5379
Diagnostic Accuracy of Polyclonal Stool Antigen for Detection of Helicobacter Pylori Infection in Children
Seyyed Ali
Jafari
Assistant Professor Of Gastroenterology, Mashhad University Of Medical Sciences, Mashhad, Iran
author
Hamidreza
Kianifar
Assistant Professor Of Gastroenterology, Mashhad University Of Medical Sciences, Mashhad, Iran
author
Ali
Khakshoor
Assistant Professor, Bojnord University Of Medical Sciences, Mashhad, Iran
author
Simin
Hiradfar
Pathologist, Mashhad Dr Sheikh Hospital, Mashhad, Iran
author
Nona
Zabolinejad
Associate Professor of Pathology, Mashhad University Of Medical Sciences, Mashhad, Iran
author
Ezzat
Khodashenas
Resident in Pediatrics, Mashhad University Of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction Helicobacter pylori infection has various clinical features. One of the most common presentations of this infection is upper abdominal pain. Complications such as gastritis, gastric ulcer, gastric carcinoma and MALT lymphoma mandate early diagnosis of H.P infection by a low cost and non invasive manner. The aim of this study was to evaluate the diagnostic role of H.P stool antigen detection as a simple and non invasive method for diagnosis of this infection. Materials and Methods Upper endoscopy with gastric biopsy was done on all patients between 6 months to 18 years old with upper abdominal pain. Stool test was done by polyclonal anti-h.p antibody. Results of stool Test were compared with results of RUT and histologic examination Results Overall 110 patients (57 boys,53 girls) were studied, 32 patients were Helicobacter pylori positive and 78 patients were Helicobacter pylori negative based on RUT and histologic examination. Stool antigen testing was positive for 39 patients. Sensitivity and specificity of stool Ag were 100% and 91% respectively Conclusion Stool Ag test has high sensitivity and negatity predictive value for diagnosis of Helicobacter pylori infection.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
158
162
https://mjms.mums.ac.ir/article_5380_37ebd04cdbad2703aed07ebf798ee06e.pdf
dx.doi.org/10.22038/mjms.2010.5380
Aseptic Meningitis after MMR Vaccination in Children of Mashhad
Mohammad Saeed
Sasan
Assistant professor of Pediatrics, Subspecialty in Pediatric Infectious Diseases, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mahboube
Haji Shamsaei
Nursing student, nursing and Midwifery School of Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction In Iran vaccination against Mumps (with Zagreb strain) has been started since 2005, and since that time the incidence of Mumps and its complications (like meningitis) has dramatically decreased. However during the 3 years after Mumps vaccination there have been frequent reports of Parotitis and Meningitis in the first few weeks after the shot. This article is the report of the authors experience about the probable adverse reactions of mumps vaccine. Materials and Methods This was prospective cross sectional observational study in Pediatric wards of Imam Reza and Dr Sheikh hospitals from August 2004 up to July 2009. In this study children who developed Parotitis or Meningitis within 0ne to six weeks after MMR vaccination were considered as probable Mumps vaccine adverse reaction. Results There were 24 cases of Meningitis (with or without Parotitis) and 16 cases of isolated Parotitis during 9 to 39 days after MMR vaccination. Male to Female ratio was 3/1 in Meningitis and 2/3 in Parotitis group. In the whole group %65 (26 ) of children were 4-6 years old and 30% of them (12 cases) were One year old, and in all of them this was the first shot of Mumps vaccine. We had a probable case of vaccine virus transmission from a 7 years old girl to her 14 years old brother. Conclusion Meningitis is a common event during one to six weeks after Mumps vaccination with the current (Zagreb) strain.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
163
168
https://mjms.mums.ac.ir/article_5381_7ad4e1833f194b09e8441aa922f5535a.pdf
dx.doi.org/10.22038/mjms.2010.5381
Comparison of Quality of Life between Hemodialysis and Peritoneal Dialysis Patients in Imam Reza and Ghaem Hospital Dialysis Centers in Mashhad
Abbas Ali
Zeraati
Assistant Professor of Nephrology,Mashhad University of Medical Sciences, Mashhad, Iran
author
Masih
Naghibi
Professor of Nephrology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mohammad javad
ojahedi
Professor of Nephrology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Shahab
Ahmad Zadeh
Medical Doctor
author
Boshra
Hasan Zamani
Nephrologists, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction Relative differences in QOL between hemodialysis (HD) and peritoneal dialysis (PD) are not clearly known. The objective of this study was to compare QOL between HD and PD patients in Emam Reza and Ghaem hospital dialysis centers. Materials and Methods We compared 40 patients (17male and23 female) on PD with 40 matched patients (20 male and 20 female) on HD. Health related 36 item short form questionnaire (SF-36) were used to assess the quality of life. For the SF-36, eight domain scores (physical functioning [PF], role limitations as a result of physical problems [RP], body pain [BP], general health perceptions [GH], social functioning [SF], role limitations as a result of emotional problems [RE], vitality [VT], mental health [MH]) were calculated. We also calculated summary Physical Component (PCS), Mental Component (MCS) and total scores of QOL. Results This study included 80 patients treated with HD and PD. The worst score was for RP dimension in both groups, and the best score was for RE in PD patients and BP in HD patients. SF-36 domains of GH, RP, VT and PCS were all significantly higher in the PD patients as compared to the HD patients. The only domain for which significant differences favoured HD patients was BP. (P < 0.05). Conclusion This study provided evidence that patients in PD treatment modality were experiencing better quality of life in SF-36 domains of GH, RP, VT, PCS in comparison to HD patients
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
169
176
https://mjms.mums.ac.ir/article_5382_a11da2c86679104fdb5daf891478cace.pdf
dx.doi.org/10.22038/mjms.2010.5382
Evaluation of Relationship between Contrast Sensitivity, Color Vision And
Visual Acuity in Diabetic Patients with and Without Retinopathy
Javad
Heravian
Ph.D. of Optometry, Associate professor of Mashhad University of Medical Sciences, Mashhad, Iran
author
Abbas
Azimi
Ph.D. of Optometry, Associate professor of Mashhad University of Medical Sciences, Mashhad, Iran
author
Shayesteh
Yasini
MSc in Optometry
author
Hadi
Ostadimoghaddam
Ph.D. of Optometry, Associate professor of Mashhad University of Medical Sciences, Mashhad, Iran
author
Abbas Ali
Yekta
Ph.D. of Optometry, Associate professor of Mashhad University of Medical Sciences, Mashhad, Iran
author
Naser
Shoeibi
Ophthalmologist, Assistant professor of Mashhad University of Medical Sciences, Mashhad, Iran
author
Habibolah
Esmaily
Epidemiolojist, Assistant professor of Mashhad University of Medical Sciences, Mashad,Iran
author
text
article
2010
per
Introduction To investigate the discriminative ability of contrast sensitivity (CS), color vision and log MAR visual acuity (VA) in detecting functional losses in diabetic participants with and without retinopathy comparing to the control group. Materials and Methods In this cross sectional study in 1388 we examined 105 patients in Mashhad Khatam- Al-Anbia Eye Hospital including 70 diabetic patients (35 with retinopathy and 35 without retinopathy) with 35 control group. Contrast sensitivity was examined by means of CSV-1000 E at 3, 6, 12 and 18 cpd respectively. Color discrimination ability was measured with the Farnsworth D-15 test and log MAR VA by Snellen chart. Results Contrast sensitivity was significantly lower in the diabetic eyes with retinopathy than in the normal eyes in all spatial frequencies. Comparing to the control group, there was a statistically significant CS loss in spatial frequencies of 3, 6, 18 cpd in the diabetic eyes without retinopathy (p<0.05). The mean log MAR VA and color vision abnormalities were significantly higher in the diabetic eyes with retinopathy than in the normal eyes or the diabetic eyes without retinopathy (p<0.001). The sensitivity and specificity of the CS test in 6 cpd were 71% and 82% which were significantly higher than other spatial frequencies. The sensitivity and the specificity of the color vision test and VA (log MAR ≥ 0.05, Snellen ≤ 9/10) were 79% and 94% respectively. Conclusion There was significant difference between the visual performance of those diabetics with retinopathy and those without. There was excellent agreement between the results of these three tests
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
177
184
https://mjms.mums.ac.ir/article_5383_c1d11937024738102f677640953c03ae.pdf
dx.doi.org/10.22038/mjms.2010.5383
Three Case of IBD or Exacerbation of IBD after Ovulation Induction
Azita
Ganji
Gastroenterologist
author
Mahmoud
Farhoodi
Professor of Internal Medicine,
Mashhad University of Medical Sciences, Mashhad, Iran
author
Kamran
Ghaffarzadegan
Clinical Pathologist
author
Akram
Bikyazdi
Resident of Internal Medicine,Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2010
per
Introduction Ulcerative colitis (UC) and Crohn’s disease (CD), collectively referred to as inflammatory bowel disease (IBD), present with different histology and cytokine profiles. While the precise mechanisms underlying the development of IBD are not known, sufficient data have been collected to suggest that it results from a complex interplay of genetic , environmental, and immunologic factors. An inappropriate mucosal immune response to normal intestinal constituents is a key feature, leading to an imbalance in local pro- and anti-inflammatory cytokines. Neutrophil and monocyte influx occurs with subsequent secretion of oxygen radicals and enzymes, leading to tissue damage. [ Case report Here we report three patients with sever symptom of IBD after ovarian stimulation. Conclusion Cytokine profile after ovarian stimulation may contribute to the altered intestinal permeability due to increase in systemic inflammatory cytokines and neutrophil or macrophage activation. In our hypothesis ovulation induction can induce inflammation in intestinal mucosa or exacerbate previous IBD by different cytokines.
medical journal of mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد
1735-4013
53
v.
3
no.
2010
189
192
https://mjms.mums.ac.ir/article_5384_fb65f4090904803a84dab9595bcc185c.pdf
dx.doi.org/10.22038/mjms.2010.5384