The effect of Lactobacillus paracasei probiotic on BDNF gene expression in the striatum of Parkinsonian model rats induced by 6-hydroxydopamine.

Document Type : Research Paper

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Master's degree, Animal Biology, Physiology, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Branch, Tehran, Iran.

10.22038/mjms.2024.71249.4232

Abstract

Introduction: Parkinson's disease (PD), a common neurodegenerative disease, is hallmarked by damage to the dopaminergic neurons of the substantia nigra and stiatum. Due to the positive role of brain-drived neutrophic factor (BDNF) in neurodegenerative disease and neuroprotective effects of probiotics, the effect of Lactobacillus paracasei probiotic bacteria on BDNF Gene Expression in the Stratuim of 6-hydroxydopamine Rat Model of Parkinson Disease was investigated in the present stud
Material and method: 30 mature male Wistar rats were divided into 3 groups: Control, Parkinson and Treatment. The rats were gavaged by saline in Control and Parkinson groups and by Lactobacillus paracasei probiotic bacteria in Treatment group for one month. The model of Parkinson’s disease was induced injection of 6-hydroxydopamine in Substantial nigra using a stereotaxic instrument. The amphetamine rotation test was taken. Quantitative Real-time RT-PCR was used to determine the changes occurred in BDNF gene expression.
Results: The results showed that BDNF gene expression increased in treatment group compared to Parkinson group and net rotations has reduced significantly as well.
Conclusion: It seems that pre-treatment of Parkinson’s model of rats with Lactobacillus paracasei probiotic bacteria has increased BDNF, which may caused by neuroprotective effects of probiotics, suggesting a novel therapy for Parkinson’s disease.

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medical journal of mashhad university of medical sciences
Volume 67, Issue 1
March and April 2024

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