Identification of Leishmania Species Causing Cutaneous Leishmaniasis by RAPD-PCR
Masoud
Mohajery
Associate professor of parasitology, Mashhad university of Medical Sciences, Mashhad, Iran
author
Homa
Hajjaran
Assistant professor of parasitology,Health school, Tehran University of Medical Sciences, Tehran, Iran
author
Ali Akbar
Shamsiyan
Assistant professor of parasitology, Mashhad university of Medical Sciences, Mashhad, Iran
author
Jalil
Tavakkol Afshari
Associate of Immunology, Mashhad university of Medical Sciences, Mashhad, Iran
author
Fatemeh
Saadabadi
MSc of Medical parasitology, Mashhad university of Medical Sciences, Mashhad, Iran
author
text
article
2008
per
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ntroduction: Leishmaniasis, especially cutaneous leishmaniasis is considered as an important health problem in many parts of Iran such as Neishabour, in Razavi Khorasan Province. Since DNA of every parasite such as every organism is specific, this facilitates extensive use of DNA for diagnosis and identification of parasite species. Among these methods, RAPD-PCR seems to be a useful method. Since Neishabour is an important focus for cutaneous leishmaniasis and so far molecular researches haven’t been done, this study was done to identify different species of leishmania parasites causing cutaneous leishmaniasis by RAPD-PCR.
Materials and Methods:In this study a total of fifty-seven patients, whom their disease confirmed by direct smear, were recruited and samples isolated and cultured in NNN medium, followed by sub-culturing in RPMI-1640. This study was approved by the local ethics. Then DNA was extracted by proteinase k and desirable samples were amplified by RAPD-PCR method, using four oligoprimers. Electrophoresis patterns from each isolate were compared with the reference strains of L.major, L. tropica and the marker.
Results:The results of this study indicated that L. tropica is the responsible parasite for causing cutaneous leishmaniasis, in Neishabour, Northeast of Iran.
Conclusion:It seems that Leishmania tropica is the only causative agent of Cutaneous Leishmaniasis in this study area. RAPD-PCR technique is a suitable tools for Leishmania characterization the in epidemiological studies.
medical journal of mashhad university of medical sciences
1735-4013
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v.
2
no.
2008
79
86
https://mjms.mums.ac.ir/article_5515_0deafd1685ee9bf44dedfda7cdefd953.pdf
dx.doi.org/10.22038/mjms.2008.5515
Serologic Evaluation of HIV-1 and Syphilis in Patients withClinical Anogenital Wart
Masoud
Maleki
Assosiat professor of Dermatology, Mashhad University of Medical sciences, Mashhad, Iran
author
Zari
Javidi
Professor of Dermatology, Mashhad University of Medical sciences, Mashhad, Iran
author
Vahid
Mashayeki
Assistant professor of Dermatology, Mashhad University of Medical sciences, Mashhad, Iran
author
Naser
Tayebi Meybodi
Assistant professor of Patology, Mashhad University of Medical sciences, Mashhad, Iran
author
Yalda
Nahidi
Resident of Dermatology, Mashhad University of Medical sciences, Mashhad, Iran
author
Habibollah
Esmaeili
Resident of Dermatology, Mashhad University of Medical sciences, Mashhad, Iran
author
Leily
Hafizi
Assistant professor of Gynecology, Mashhad University of Medical sciences, Mashhad, Iran
author
text
article
2008
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ntroduction:Genital infection with human papilloma virus (HPV) is the most common sexually transmitted disease in the United States. Patients having genital warts are at excessive risk of HIV and syphilis. Uninformed patients with high risk behaviors can help spread this condition in society. Therefore, CDC recommends HIV and syphilis screening for those with a sexual transmitted disease or a sexual risk factor. But this strategy is rarely considered in Iran. The aim of this study was to evaluate the cost-effectiveness of HIV and syphilis screening tests in patients with genital wart.
Materials and Methods:In this case-control study, 100 patients with genital wart referring to Dermatology Department of Imam-Reza Hospital (August 2005 - August 2007) were included as the case group. This study was approved by the local ethics. The Control group was the same in number, age average, sex and marital status. All 200 patients studied considering HIV or syphilis infection. Finally, collected data were statistically analyzed by SPSS software, using χ2and student T test.
Results:Male to female ratio was 0.87. Majority of patients aged 30-39 years. Two patients were HIV positive and only one had Syphilis, without significant difference with the control group (Pv =0.29, Pv =0.50).
Conclusion: Screening is not cost effective in patients with asymptomatic anogenital warts. Since both HIV positive cases were intravenous drug abusers, this study emphasized on HIV and syphilis screening in patients with high risk behavior.
medical journal of mashhad university of medical sciences
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2
no.
2008
87
94
https://mjms.mums.ac.ir/article_5516_7a5f9cb873cd8556d01d8190aec00782.pdf
dx.doi.org/10.22038/mjms.2008.5516
Frequency of Dermatophytoses
Seyyed Ali Akbar
Shamsian
Assistant professor of Parasitology, Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
author
Mohammad Javad
Yazdan Panah
Assistant professor of Dermatology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Monireh
Mokhtari Amir Majdi
Associate professor of Parasitology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mahdi
Moradi Marjaneh
General Practitioner, Research Instructor, Academic Center for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
author
Sara
Saboori Rad
Resident of Dermatology, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2008
per
Introduction:Dermatophytosis is a fungal infection caused by dermatophytes, which have the ability to invade keratinized tissues such as hair, nail and skin. Epidemiology of the disease depends on age as well as prevailing hygiene, socioeconomic status and cultural conditions.
Materials and Methods:This descriptive study was conducted from March 2006 to March 2007 at Mashhad University of Medical Sciences, on 599 patients with suspected cutaneous fungal infection at Dermatology Clinic of Ghaem Hospital Mashhad University of Medical Sciences, Mashhad, Iran. Direct examination of smears and culture on SCC were used to diagnose the infection in mycology labs of Ghaem Hospital and Mashhad branch of ACECR.
Results:Overall, 384 patients had fungal infection (64.1%), out of which 137 cases were suffering from dermatophytosis. Numbers of male and female cases were 91 and 46 respectively (p= 0.002). Maximum infection rate found in 21-30 years age group and tinea corporis was the most common dermatophytic infection (56.2%) followed by tinea cruris (20.4%) and tinea pedis (13.8%). The frequencies of etiological agents isolated from patients were as follows: Tricophyton rubrum, 44.5%; Epidermophyton floccosum, 21.9%; Tricophyton mentagrophyte, 11%; Tricophyton verrucosum, 7.3% and Microsporum canis, 7.3%.
Conclusion:Since high prevalence of dermatophytosis reported in this study, public education on the potential risk factors, diagnosis and treatment of the disease are recommended and more studies to determine the main agents of dermatophytosis in other regions of our country are necessary.
medical journal of mashhad university of medical sciences
1735-4013
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v.
2
no.
2008
95
102
https://mjms.mums.ac.ir/article_5517_773cb6cbea0b249a1483ce3b98d22832.pdf
dx.doi.org/10.22038/mjms.2008.5517
Evaluation of Serum Zinc and Copper Concentrations in Epileptic Patients
Habibollah
Nemati Karimuy
Assistant professor of Neurology, Medical School, Mashhad university of Medical Sciences, Mashhad, Iran
author
Musareza
Hajzadeh
Associate professor of Metabolism and endocrine Physiology, Medical School, Mashhad, Iran
author
Zahra
Daei
BSc, Biology, Mashhad university of Medical Sciences, Mashhad, Iran
author
text
article
2008
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ntroduction: Discovering ethiology of epilepsy is inevitable to find effective treatment methods. The aim of this study was to evaluate serum zinc and copper concentrations in patients with epilepsy.
Materials and Methods: This study was done in 2006. A total ofsixty patients (25 males, 35 females; mean age 22.43±1.18 years) with epilepsy and 60 age-matched healthy subjects (25 males, 35 females; mean age 25.27±1.091 years) enrolled in this study. This study was approved by the local ethics. Serum concentrations of Zn and Cu assessed for all subjects in both groups with an atomic absorption spectrophotometer. Results were demonstrated as Mean± SEM for Cu (μg/lit) and for Zn (μg/dl). The results compared by unpaired t-test and when p<0.05, the differences considered to be significant.
Results: Mean copper level was 947.98±30.39 μg/lit and 967.41±28.25 μg/lit in the case and control group, respectively. The serum concentration of zinc was 86.81±1.55 μg/dl in patients and 94.48 ± 11.11 in the control group μg/dl. The differences between the two groups was insignificant (p>0.05).
Conclusion: In contrast with some other studies which showed a difference in serum concentrations of Cu and Zn in epileptic patients, our data demonstrated that concentrations of serum zinc and copper in epileptic patients are not statistically different from the control group. Therefore, it is suggested that serum levels of Zn and Cu are not the strong determinant factors involved in inducing epilepsy; thus, it can be concluded that epileptic episodes in human may not be related to the serum levels of both trace elements Cu and Zn.
medical journal of mashhad university of medical sciences
1735-4013
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2
no.
2008
103
108
https://mjms.mums.ac.ir/article_5518_2a37764d211fa3852f845e3f1bc83938.pdf
dx.doi.org/10.22038/mjms.2008.5518
Evaluation of Patients with Inflammatory Myopathies
Mohamad Mahdi
Etemadi
Professor of Neurology. Mashhad University of Medical science, Mashhad, Iran
author
Reza
Sadre Nabavi
Professor of Neurology and Neuropathology. Mashhad University of Medical science, Mashhad, Iran
author
Payam
Sasannejad
Assistant Professor of Neurology. Mashhad University of Medical science, Mashhad, Iran
author
Mohammad Taghi
Farzadfard
Neurologist. Mashhad University of Medical science, Mashhad, Iran
author
text
article
2008
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ntroduction:Inflammatory Myopathies are a group of acquired diseases in which most important finding is subacute progressing symmetrical proximal muscle weakness and fatigue with mononuclear cells infiltration in the muscle tissue along with degeneration and regeneration of muscle fibers and elevation of the muscle enzymes.
In nonspecific myositis, the important point is the prominent infiltration of the macrophages rather than lymphocytes in the muscle tissue, and intact fibers with infiltration are less common.
Materials and Methods: In this retrospective and descriptive study, files and pathological slides of patients with myositis, hospitalized in the neurologic ward of Ghaem hospital or outpatients visited the neuropathology center from 1996 to 2006, were again evaluated. This study was approved by the local ethics.
Results:Overall, 39 patients with inflammatory myopathy diagnosis admitted during this period, of which were 56.4% males and 43.6% females. The average age of the women and men was 31 and 35.5 years respectively.
Dysphagia was see in 33.3%, tenderness and muscle pain in 41%, Reynoud’s phenomen in 7.6%, elevated muscle enzymes in 82%, lung involvement in 5.1%, heart involvement in 5.1%, positive ANA in 5.1% and elevated ESR in 53.8% of the patients.
Thirty-nine patients were evaluated in the neuropathology ward and amoung this group 36 cases had nonspecific myositis and 3 patients had polymyositis.
Conclusion:In this study it is shown that polymyositis is a rare disease, and the presence of inflammation in muscle biopsy, elevated muscle enzymes and weakness of girdle muscles are not necessary for the polymyositis diagnosis confirmation. However, most of these cases are nonspecific myositis which will not respond to corticosteroid therapy.
medical journal of mashhad university of medical sciences
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2
no.
2008
109
114
https://mjms.mums.ac.ir/article_5519_e820d9483b47d6b45190b92a1af45e32.pdf
dx.doi.org/10.22038/mjms.2008.5519
Evaluation of the Clinical Features , Diagnostic Procedures and Mortality of Acute PulmonaryThromboembolism
Pulmonary and Ttuberculosis Research Center, Mashhad of Medical Sciences University
Javad
Ghasemieh
Assistant Professorof Internal Medicine, Mashhad University of Medial Sciences, Mashhad, Iran
author
Fariba
Rezaeetalab
Assistant Professorof Internal Medicine, Mashhad University of Medial Sciences, Mashhad, Iran
author
text
article
2008
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ntroduction: Acute pulmonary Thromboembolism from deep venous thrombosis is an extremely important cause of morbidity and mortality . The aims of this study were evaluation of clinical features, diagnostic approaches and especially, mortality of pulmonary thromboembolism.
Materials and Methods: In this descriptive study, 142 patients with thromboembolism evaluated from 2003 to 2007 at Internal Medicen Department, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran. This study was approved by the local ethics. Demographic characteristics, diagnostic approaches and mortality of pulmonary thromboembolism recorded. Data analyzed with Spss 11/5 software.
Results: In 142 patients, dyspnea 80/3%, chest pain 38/7%, cough 31/7%, fever 30/3%, hemoptysis 18/3%, deep vein thrombosis in right lower limb 20/4%, deep vein thrombosis in left lower limb in 17/6% were recorded. Among 142 patients, mortality was seen in 25 cases.
Conclusion: The results of this study are the same as others, in spite of fever that is the fourth manifestation. Longstanding bed rest is the most risk factor for pulmonary thromboembolism. So this risk factor is preventable. Lung perfusion and Doppler sonography were diagnostic. Unfortunately, mortality in our study is more than other studies and it is estimately , doubled.
medical journal of mashhad university of medical sciences
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51
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2
no.
2008
115
120
https://mjms.mums.ac.ir/article_5520_a971a0ba54114b39e99ce85d8d939193.pdf
dx.doi.org/10.22038/mjms.2008.5520
Evaluation of Patency Rate for Arteriovenous Fistulas Using Superficial Basilic Vein Transposition in Dialyzing End Stage Renal Disease Patients
Vascular Surgery Research Center Mashhad University of
Mohammad-Hadi
Saeed Modaghegh
Associate professor of vascular surgery, Imam Reza Hospital,Mashhad University of Medical Sciences, Mashhad, Iran
author
Hassan
Ravari
Assistant professor of vascular surgery, Imam Reza Hospital,Mashhad University of Medical Sciences, Mashhad, Iran
author
GholamHossein
Kazemzadeh
Assistant professor of vascular surgery, Imam Reza Hospital,Mashhad University of Medical Sciences, Mashhad, Iran
author
Hoda
Khallaghi
General practitioner, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2008
per
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ntroduction:Long term vascular access has become more important as patients live longer on dialysis. Improved survival on hemodialysis has resulted in an increasing number of patients with failed vascular access, so an increasing number of options for vascular access have to be added to the surgeon’s armamentarium. In a number of patients, basilic vein transposition is an often overlooked means of arteriovenous access that has a superior patency compared with bridge fistulas using polytetrafluoroethylene (PTFE). And this brought vascular surgeons of Imam-Reza hospital to use this technique in patients with complications in normal fistulas. This article evaluates the survival rate of these fistulas and compares them with patency rates reported worldwide.
Materials and Methods: This study was conducted on, 25 End Stage Renal Disease (ESRD) patients, undergone superficial basilic vein transposition from 2002 till 2005. In these patients, superficial basilic vein transposition method was performed for an arteriovenous fistula (AVF). Demographic data were collected using medical records, and then patients were examined clinically. Patency rate was evaluated using life tables by SPSS version 13 and compared with similar results reported worldwide.
Results: Overall 70.8% were male patients and 29.2% were female. Hypertension was seen in 87.5% of cases and 37.5% suffered from diabetes. Seventy four percent of our cases underwent basilic vein transposition after a failing AVF, one case after a rejected transplant and one after a failed graft. The longest patency rate was 30 months and the shortest was 4 months. The patency rate for the first 6 months was 86% and after one year 77% and after 26 months 58%.
Conclusion: The one year patency rate reported for fistulas by basilica vein transposition method is 90-60% which justifies the results of this article (62.5%). these results suggest that this method can be used as a new access line for dialyzing ESRD patients before using graft fistulas.
medical journal of mashhad university of medical sciences
1735-4013
51
v.
2
no.
2008
121
126
https://mjms.mums.ac.ir/article_5521_904e100283126fe53787532c264af635.pdf
dx.doi.org/10.22038/mjms.2008.5521
Lumbosacral Tethered Cord Syndrome
(A Review of 27 Cases )
Fariborz
Samini
Associate Professor of Neurosurgery Mashhad University of Medical Sciences, Mashhad, Iran
author
Housein
Mashhadinejad
Associate Professor of Neurosurgery Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2008
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ntroduction: Tethered cord syndrome is a neurological disorder that is accompanied by limited movement of spinal cord within the spinal column. This study aimed to describe clinical features, surgery treatment and long term outcomes in these patients.
Material and Methods: This prospective study carried out from 1997 to 2007 on we selected 27 patients with lumbosacral tethered cord syndrome, neurological findings and positive MRI. Operation was performed clinical and neurological and imaging findings and their etiologies were recorded. Follow up was achieved in three periods for studying and reviewing of their neurological and scoliosis recovery. Data analyzed by descriptive statistics and frequency distributes tables by SPSS software.
Results: Sixteen patients were male and 11 were female. 22 cases were younger than 7 and 5 other cases were between 17 to 33. After 3 years, most of disorders were recovered. The best results were due to LBP, motoric disorder, dysesthesia and muscle cramps in lower limbs. Tree Patients had CSF leakage and one patient had pseudomeningocele after operation.
Conclusion: Surgery is recommended for resection of lesion, cord detethering or resection of tight filum terminal and adhesions. Results of surgical treatment are excellent especially in younger patients. In children, surgery is achieved for prevention of progressive neurological disorders. Cord detethering in adults, diminishes the size of cord cysts and prevents of progressive clinical and neurological disorders.
medical journal of mashhad university of medical sciences
1735-4013
51
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2
no.
2008
127
130
https://mjms.mums.ac.ir/article_5522_fac537f9fcb93c628fb1e8fa392d1d61.pdf
dx.doi.org/10.22038/mjms.2008.5522