Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Phenotypic and genotypic evaluation of fluoroquinolones resistance in Klebsiella pneumoniae isolates from urinary tract infections in Bojnourd city

Document Type : Research Paper

Authors
Majid Jamshidian-Mojaver 1
Mohadese Amiri 2
Hamidreza Farzin 1
1 Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.
2 Master of Bacteriology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
10.22038/mjms.2020.16893
Abstract
Introduction: Urinary tract infection (UTI) is one of the most common bacterial infections, accounting for a significant proportion of hospital clients (about 30-40%). The emergence of resistance to cephalosporins, fluoroquinolones and trimethoprim-sulfamethoxazole which were often used to treat general and nosocomial infections, delayed effective treatment of these diseases and subsequently resulted in increased infections and mortality of patients.
Methods: In this study, 30 specimens of positive cultures of urinary tract infection which were referred to Imam Reza Hospital laboratory in Bojnourd were studied. The resistance and susceptibility of the isolates were determined by disc diffusion method. The presence of QnrA gen was assessed by molecular methods with specific primers.
Results: The phenotypic resistance of isolates to nalidixic acid, levofloxacin and ciprofloxacin were 43.33%, 26.66% and 40%, respectively. Also, genotypic analysis of the isolates showed 6.66% of the studied genes.
Conclusion: Based on the results of the present study, it can be said that resistance to antibiotics belonging to the fluoroquinolones is not yet widely high.
Keywords
UTI
Klebsiella pneumonia
Antibiotic resistance
Fluoroquinolonese
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Medical Journal of Mashhad university of Medical Sciences
Volume 63, Issue 3
July and August 2020
Pages 2335-2340