The effect of endurance training and probiotic supplementation on MMP9 gene expression in the liver tissue of steatosis rats

Document Type : Research Paper

Authors

1 Lecturer at Farabi University of Science and Arts, Tehran, Iran

2 Assistant Professor of Exercise Physiology, Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.(Corresponding Auther)

3 MSc in Exercise Physiology, Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction: Fatty liver disease is the most important reason for chronic liver diseases, from simple fatty liver to advanced stages such as liver fibrosis, cirrhosis and liver cancer. In this study, the effect of moderate intensity endurance exercise and probiotic consumption on MMP 9 gene expression in an animal model of steatosis was investigated.
Method: This study is an experimental study of laboratory type. 32 male Wistar rats were divided into 4 groups of 8: healthy group, fatty liver group, fatty liver + probiotic group, fatty liver + probiotic group + moderate intensity endurance exercise and were tested for 8 weeks. Endurance training was done for 8 weeks, 5 days a week, at 18-28 meters per minute. The statistical calculation of this study was done using the one-way analysis of variance test and, in case of a significant difference, the Bonferroni post hoc test to determine the location of the difference between groups was done with SPSS 25 software.
Results: Endurance training and probiotic consumption have a significant difference on the expression of the MMP-9 gene in the animal model of fatty liver (P=0.000) and decrease the expression of MMP-9.
Conclusion: According to the results of the study, it seems that endurance training along with probiotic consumption can make patients with fatty liver resistant to the damage caused by this disease.

Keywords

References

Fabbrini E, Sullivan S, Klein S. Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and
clinical implications. Hepatology. 2010 Feb;51(2):679-89. doi: 10.1002/hep.23280. PMID: 20041406; PMCID:
PMC3575093.
2. Pouwels S, Sakran N, Graham Y, Leal A, Pintar T, Yang W, Kassir R, Singhal R, Mahawar K, Ramnarain D.
Non-alcoholic fatty liver disease (NAFLD): a review of pathophysiology, clinical management and effects of weight
loss. BMC Endocr Disord. 2022 Mar 14;22(1):63. doi: 10.1186/s12902-022-00980-1. PMID: 35287643; PMCID:
PMC8919523.
3. Hosseini M, Eftekhar B, Riyahi Malayeri S. Effect of Interval Training with Curcumin Consumption on Some
Adipokines in Menopausal Obese Rats. JRUMS. 2017; 16 (6) :505-516URL: http://journal.rums.ac.ir/article-1-3644-
en.html.
4. Hedayati S, Riyahi Malayeri S, Hoseini M. The Effect of Eight Weeks of High and Moderate Intensity Interval
Training Along with Aloe Vera Consumption on Serum Levels of Chemerin, Glucose and Insulin in Streptozotocininduced Diabetic Rats: An Experimental Study. JRUMS. 2018; 17 (9) :801-814.URL: http://journal.rums.ac.ir/article-1-
4209-en.html.
5. Asgari Hazaveh D, Riyahi Malayeri S, Babaei S. Effect of Eight Weeks High Intensity Interval Training and
Medium Intensity Interval Training and Aloe vera Intake on Serum Vaspin and Insulin Resistance in Diabetic Male
Rats. J Arak Uni Med Sci. 2018; 20 (11) :67-75
URL: http://jams.arakmu.ac.ir/article-1-5406-en.html.
6. Maurice J, Manousou P. Non-alcoholic fatty liver disease. Clin Med (Lond). 2018 Jun;18(3):245-250. doi:
10.7861/clinmedicine.18-3-245. PMID: 29858436; PMCID: PMC6334080.
7. Zhang CY, Yuan WG, He P, Lei JH, Wang CX. Liver fibrosis and hepatic stellate cells: Etiology, pathological
hallmarks and therapeutic targets. World J Gastroenterol. 2016 Dec 28;22(48):10512-10522. doi:
10.3748/wjg.v22.i48.10512. PMID: 28082803; PMCID: PMC5192262.
8. Wang WM, Xu XS, Miao CM. Kupffer Cell-Derived TNF-α Triggers the Apoptosis of Hepatic Stellate Cells
through TNF-R1/Caspase 8 due to ER Stress. Biomed Res Int. 2020 Aug 2;2020:8035671. doi: 10.1155/2020/8035671.
PMID: 32802876; PMCID: PMC7421237.
9. Zommiti M, Feuilloley MGJ, Connil N. Update of Probiotics in Human World: A Nonstop Source of
Benefactions till the End of Time. Microorganisms. 2020 Nov 30;8(12):1907. doi: 10.3390/microorganisms8121907.
PMID: 33266303; PMCID: PMC7760123.
10. Mohammadi, S., Rostamkhani, F., Riyahi Malayeri, S. et al. High-intensity interval training with probiotic
supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis. Sport Sci
Health 18, 491–497 (2022). https://doi.org/10.1007/s11332-021-00829-5.
11. Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of Action of Probiotics. Adv Nutr. 2019 Jan
1;10(suppl_1):S49-S66. doi: 10.1093/advances/nmy063. Erratum in: Adv Nutr. 2020 Jul 1;11(4):1054. PMID:
30721959; PMCID: PMC6363529.
12. Fijan S. Microorganisms with claimed probiotic properties: an overview of recent literature. Int J Environ Res
Public Health. 2014 May 5;11(5):4745-67. doi: 10.3390/ijerph110504745. PMID: 24859749; PMCID: PMC4053917.
13. van der Windt DJ, Sud V, Zhang H, Tsung A, Huang H. The Effects of Physical Exercise on Fatty Liver
Disease. Gene Expr. 2018 May 18;18(2):89-101. doi: 10.3727/105221617X15124844266408. Epub 2017 Dec 6. PMID:
29212576; PMCID: PMC5954622.
14. Riyahi Malayeri S, Azadniya A, Rasaee M J. Effect of eight-week High intensity interval training nd
resveratrol intake on serum adiponectin and resistin in type 2 diabetic rats. Ijdld 2019; 18 (1) :8-1. URL:
http://ijdld.tums.ac.ir/article-1-5708-en.html.
15. Mirea AM, Tack CJ, Chavakis T, Joosten LAB, Toonen EJM. IL-1 Family Cytokine Pathways Underlying
NAFLD: Towards New Treatment Strategies. Trends Mol Med. 2018 May;24(5):458-471. doi:
10.1016/j.molmed.2018.03.005. Epub 2018 Apr 14. PMID: 29665983; PMCID: PMC5939989.
16. Farazandeh Nia, D., Hosseini, M., Riyahi Malayeri, S., Daneshjoo, A. Effect of Eight Weeks of Swimming
Training with Garlic Intake on Serum Levels of IL-10 and TNF-α in Obese Male Rats. Jundishapur Scientific Medical
Journal, 2018; 16(6): 665-671. doi: 10.22118/jsmj.2018.57830.
17. Riyahi Malayeri S, Abdolhay S, Behdari R, Hoseini M. The combined effect of resveratrol supplement and
endurance training on IL-10 and TNF- α in type 2 diabetic rats. RJMS. 2019; 25 (12) :140-149. URL:
http://ijdld.tums.ac.ir/article-1-5708-en.html.
18. Wang Y, Kirpich I, Liu Y, Ma Z, Barve S, McClain CJ, Feng W. Lactobacillus rhamnosus GG treatment
potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver
injury. Am J Pathol. 2011 Dec;179(6):2866-75. doi: 10.1016/j.ajpath.2011.08.039. Erratum in: Am J Pathol. 2012
Jan;180(1):429-30. PMID: 22093263; PMCID: PMC3260853.
19. Ipsen DH, Lykkesfeldt J, Tveden-Nyborg P. Molecular mechanisms of hepatic lipid accumulation in nonalcoholic fatty liver disease. Cell Mol Life Sci. 2018 Sep;75(18):3313-3327. doi: 10.1007/s00018-018-2860-6. Epub
2018 Jun 23. PMID: 29936596; PMCID: PMC6105174.
20. Rai R, Saraswat VA, Dhiman RK. Gut microbiota: its role in hepatic encephalopathy. J Clin Exp Hepatol.
2015 Mar;5(Suppl 1):S29-36. doi: 10.1016/j.jceh.2014.12.003. Epub 2014 Dec 16. PMID: 26041954; PMCID:
PMC4442863.
21. Tang Y, Huang J, Zhang WY, Qin S, Yang YX, Ren H, Yang QB, Hu H. Effects of probiotics on nonalcoholic
fatty liver disease: a systematic review and meta-analysis. Therap Adv Gastroenterol. 2019 Sep
25;12:1756284819878046. doi: 10.1177/1756284819878046. PMID: 31598135; PMCID: PMC6764034.
22. Duseja A, Acharya SK, Mehta M, Chhabra S; Shalimar, Rana S, Das A, Dattagupta S, Dhiman RK, Chawla
YK. High potency multistrain probiotic improves liver histology in non-alcoholic fatty liver disease (NAFLD): a
randomised, double-blind, proof of concept study. BMJ Open Gastroenterol. 2019 Aug 7;6(1):e000315. doi:
10.1136/bmjgast-2019-000315. PMID: 31423319; PMCID: PMC6688701.
23. Elham Sadat Mousavi, Mohammad Reza Asad, Ali Barzegari, Mahbobeh Gholizadeh Ahangari. Effect of
Continuous Endurance Training on mRNA Expression of Interferon Gamma and Matrix Metalloproteinase-9 in Male
Elder Rats. Hormozgan medical journal. 2018;22(3): e87214. doi: 10.5812/hmj.87214.
24. Chen WK, Tsai YL, Shibu MA, Shen CY, Chang-Lee SN, Chen RJ, Yao CH, Ban B, Kuo WW, Huang CY.
Exercise training augments Sirt1-signaling and attenuates cardiac inflammation in D-galactose induced-aging rats.
Aging (Albany NY). 2018 Dec 23;10(12):4166-4174. doi: 10.18632/aging.101714. PMID: 30582744; PMCID:
PMC6326662.
25. de Gregorio E, Colell A, Morales A, Marí M. Relevance of SIRT1-NF-κB Axis as Therapeutic Target to
Ameliorate Inflammation in Liver Disease. Int J Mol Sci. 2020 May 29;21(11):3858. doi: 10.3390/ijms21113858.
PMID: 32485811; PMCID: PMC7312021.
26. Wang X, Yang J, Lu T, Zhan Z, Wei W, Lyu X, Jiang Y, Xue X. The effect of swimming exercise and diet on
the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression. Aging (Albany NY). 2020
Jun 9;12(11):11085-11099. doi: 10.18632/aging.103323. Epub 2020 Jun 9. PMID: 32518216; PMCID: PMC7346084.
27. Lo Presti R, Hopps E, Caimi G. Gelatinases and physical exercise: A systematic review of evidence from
human studies. Medicine (Baltimore). 2017 Sep;96(37):e8072. doi: 10.1097/MD.0000000000008072. PMID:
28906407; PMCID: PMC5604676.
medical journal of mashhad university of medical sciences
Volume 64, Issue 6 - Serial Number 6
March and April 2022
Pages 4361-4368

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