Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Investigating the level of anti-Helicobacter pylori antibodies in infected and non-infected patients with this bacterium

Document Type : Research Paper

Authors
Abdollah Safikhani mahmoodzadeh 1
Elham Moazamian 2
Seyedeh azra Shamsdin 3
Golam abas Kaydani 4
1 Department of Microbiology, Faculty of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
2 Department of Microbiology, College of Science, Agriculture and Modern Technologies, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
3 Gasteroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Laboratory Sciences, School of Allied Medical Sciences, Ahvaze Jundishapur University of Medical Sciences, Ahvaze Iran.
10.22038/mjms.2022.66902.3961
Abstract
Introduction: Helicobacter pylori is a microaerophilic Gram-negative bacterium that is usually found in the stomach. The presence of this bacteria in the stomach can lead to gastritis and decrease gastric acid production. Also, studies have reported the relationship between this infection and gastric cancer. Early diagnosis of infection with this bacterium is crucial in its treatment. This study was conducted with the aim of investigating the level of anti-H. pylori antibodies in infected and non-infected patients.
Methods: A total of 85 patients with symptoms including 46 Hp+ and 39 Hp- samples were included in the study. Finally, the serum level of IgG and IgA antibodies was measured using an ELISA kit (Roche - Germany).
Results: The average age of the studied subjects was 43.83 ± 13.41 and 41.28 ± 12.65 in the Hp+ and Hp- groups, respectively. The Hp+ group included 17 females and 29 males; the Hp- group included 22 females and 17 males. No significant relationship between age and gender was observed in the two groups. Data analysis found that IgG and IgA levels were significantly increased in people with H. pylori infection compared to Hp- people (p<0.001). Also, the results showed no significant relationship between the level of antibodies and the gender of thepeople.
Conclusion: According to the results obtained from this research, H. pylori infection can occur at any age and gender. Considering the high prevalence of this disease and the economic and time costs, it seems necessary to conduct more studies on itand its causes.
Keywords
Helicobacter pylori
antibody
ELISA
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Medical Journal of Mashhad university of Medical Sciences
Volume 66, Issue 1
May and June 2023
Pages 74-82