Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

The effect of eight weeks of high intensity interval training on the expression of 12.13.diHome, SREBP-1 genes and body composition in obese vistar rat

Document Type : Research Paper

Authors
Mohsen Akbari 1
Amir Rashid lamir 2
Nahid Bijeg 2
Seysd Alireza Hoseini Kakhk 3
1 PhD student of biochemistry and sports metabolism. Faculty of Sports Sciences, Ferdowsi University of Mashhad. Mashhad, Iran
2 Professor of biochemistry and sports metabolism. Faculty of Sports Sciences, Ferdowsi University of Mashhad. Mashhad, Iran
3 Associate Professor of Biochemistry and Sports Metabolism. Faculty of Sports Sciences, Ferdowsi University of Mashhad. Mashhad, Iran
10.22038/mjms.2023.73131.4337
Abstract
Abstract:
Introduction: Adipose tissue is one of the tissues that affects the process of obesity. The aim of the present study was the effect of eight weeks of high intensity interval training on the expression of 12.13.diHome, SREBP-1 genes and body composition of non-obese rats.
working method; In the present study, 18 obese Wistar rats, aged eight weeks and weighing 343.88±42.20 grams, were randomly divided into two groups; high intensity interval training (10 heads) and control (8 heads). The rats of the experimental group performed high intensity interval training with an intensity of 85 to 90% of the maximum speed for eight weeks, five sessions a week, and Real Time-PCR method was used to measure the expression of 12.13.diHome and SREBP-1 genes. Became. Statistical methods of independent t-test and dependent t-test were used to check the statistical data at a significance level of P ≤ 0.05.
Results; The results of the present study showed that high intensity interval training led to a significant increase in the expression of the 12.13.diHome gene (P=0.01) and a decrease in the expression of the SREBP-1 gene. The same embryo caused a decrease in the weight (P=0.001) and body mass index (P=0.001) of the rats.
conclusion; According to the results of this research, it is suggested to use high intensity interval training that increase intramuscular lipolysis and decrease hepatic lipogenesis; To be used to reduce fat mass
Keywords
HIIT
12.13.diHome
SREBP-1
body composition
obesity
Subjects
  1. Jin X, Qiu T, Li L, Yu R, Chen X, Li C, et al. Pathophysiology of obesity and its associated diseases. Acta Pharmaceutica Sinica B. 2023.
  2. Fei X, Huang J, Li F, Wang Y, Shao Z, Dong L, et al. The Scap-SREBP1-S1P/S2P lipogenesis signal orchestrates the homeostasis and spatiotemporal activation of NF-κB. Cell Reports. 2023;42(6).
  3. Gao Y, Liu J, Hao Z, Sun N, Guo J, Zheng X, et al. Baicalin ameliorates high fat diet‐induced nonalcoholic fatty liver disease in mice via adenosine monophosphate‐activated protein kinase‐mediated regulation of SREBP1/Nrf2/NF‐κB signaling pathways. Phytotherapy Research. 2023.
  4. Zhou Q, Kong D, Li W, Shi Z, Liu Y, Sun R, et al. LncRNA HOXB-AS3 binding to PTBP1 protein regulates lipid metabolism by targeting SREBP1 in endometrioid carcinoma. Life Sciences. 2023;320:121512.
  5. Lu Y, Zhang C, Song Y, Chen L, Chen X, Zheng G, et al. Gallic acid impairs fructose-driven de novo lipogenesis and ameliorates hepatic steatosis via AMPK-dependent suppression of SREBP-1/ACC/FASN cascade. European Journal of Pharmacology. 2023;940:175457.
  6. Moon BR, Han JS. HM-chromanone suppresses lipid accumulation by modulating AMPK/SREBP-1c pathway in ob/ob mice. Journal of Functional Foods. 2023;104:105538.
  7. Oh S, Shida T, Yamagishi K, Tanaka K, So R, Tsujimoto T, et al. Moderate to vigorous physical activity volume is an important factor for managing nonalcoholic fatty liver disease: a retrospective study. Hepatology. 2015;61(4):1205-15.
  8. Cintra DE, Ropelle ER, Vitto MF, Luciano TF, Souza DR, Engelmann J, et al. RETRACTED: Reversion of hepatic steatosis by exercise training in obese mice: the role of sterol regulatory element-binding protein-1c. Elsevier; 2012.
  9. Yang J, Sáinz N, Félix-Soriano E, Gil-Iturbe E, Castilla-Madrigal R, Fernández-Galilea M, et al. Effects of long-term DHA supplementation and physical exercise on non-alcoholic fatty liver development in obese aged female mice. Nutrients. 2021;13(2):501.
  10. Ebrahimi M, Fathi R, Ansari Pirsarii Z, Talebi-Garakani E. Relative gene expression of key genes involved in lipid metabolism, following high fat diet and moderate and high intensity aerobic training in rat’s liver. Sport Physiology. 2017;9(34):201-16.
  11. Azarbayjani M, Banaeifar A, Arshadi S. The Effect of Aerobic Training and Adenosine on the Expression of SREBP-1C and A1 Receptor in Hepatic Fat-fed Rats. Iranian Journal of Nutrition Sciences & Food Technology. 2019;14(1):1-9.
  12. Jeong S. Regulation of PPAR and SREBP-1C Through Exercise in White Adipose Tissue of Female C57BL/6J Mice. 대한의생명과학회지. 2012;18(3):227-36.
  13. Haun CT, Mobley CB, Vann CG, Romero MA, Roberson PA, Mumford PW, et al. Soy protein supplementation is not androgenic or estrogenic in college-aged men when combined with resistance exercise training. Scientific reports. 2018;8(1):11151.
  14. Asano M, Iwagaki Y, Sugawara S, Kushida M, Okouchi R, Yamamoto K, et al. Effects of Japanese diet in combination with exercise on visceral fat accumulation. Nutrition. 2019;57:173-82.
  15. Dobrzyn P, Pyrkowska A, Jazurek M, Szymanski K, Langfort J, Dobrzyn A. Endurance training-induced accumulation of muscle triglycerides is coupled to upregulation of stearoyl-CoA desaturase 1. Journal of Applied Physiology. 2010;109(6):1653-61.
  16. Nadeau KJ, Ehlers LB, Aguirre LE, Moore RL, Jew KN, Ortmeyer HK, et al. Exercise training and calorie restriction increase SREBP-1 expression and intramuscular triglyceride in skeletal muscle. American Journal of Physiology-Endocrinology and Metabolism. 2006;291(1):E90-E8.
  17. Ikeda S, Miyazaki H, Nakatani T, Kai Y, Kamei Y, Miura S, et al. Up-regulation of SREBP-1c and lipogenic genes in skeletal muscles after exercise training. Biochemical and biophysical research communications. 2002;296(2):395-400.
  18. Zarei F, Sherafati Moghadam M, Shabani M, Jokar M. the effects of 4 weeks high intensity interval training on mammalian rapamycin target protein (mtor) and sterol transcription factor regulatory protein-1 (srebp1) proteins content in diabetics obese rats adipose tissue. Iranian Journal of Diabetes and Metabolism. 2020;19(1):26-35.
  19. Shahouzehi B, Masoumi-Ardakani Y, Nazari-Robati M, Aminizadeh S. The Effect of High-intensity Interval Training and L-carnitine on the Expression of Genes Involved in Lipid and Glucose Metabolism in the Liver of Wistar Rats. Brazilian Archives of Biology and Technology. 2022;66.
  20. Egan B, Sharples AP. Molecular responses to acute exercise and their relevance for adaptations in skeletal muscle to exercise training. Physiological Reviews. 2023;103(3):2057-170.
  21. Hildreth K, Kodani SD, Hammock BD, Zhao L. Cytochrome P450-derived linoleic acid metabolites EpOMEs and DiHOMEs: a review of recent studies. The Journal of nutritional biochemistry. 2020;86:108484.
  22. Lynes M. Leiria LO Lundh M Bartelt A Shamsi F Huang TL Takahashi H Hirshman MF Schlein C Lee A et al. 2017 The cold-induced lipokine 12, 13-diHOME promotes fatty acid transport into brown adipose tissue. Nature Medicine.631:637.
  23. Badi I, Antoniades C. Brown Adipose Tissue and the Take (12, 13-di) HOME Message to the Heart. Am Heart Assoc; 2021. p. 160-2.
  24. Pinckard KM, Shettigar VK, Wright KR, Abay E, Baer LA, Vidal P, et al. A novel endocrine role for the BAT-released lipokine 12, 13-diHOME to mediate cardiac function. Circulation. 2021;143(2):145-59.
  25. Foryst-Ludwig A, Kreissl MC, Benz V, Brix S, Smeir E, Ban Z, et al. Adipose tissue lipolysis promotes exercise-induced cardiac hypertrophy involving the lipokine C16: 1n7-palmitoleate. Journal of Biological Chemistry. 2015;290(39):23603-15.
  26. Stanford KI, Lynes MD, Takahashi H, Baer LA, Arts PJ, May FJ, et al. 12, 13-diHOME: an exercise-induced lipokine that increases skeletal muscle fatty acid uptake. Cell metabolism. 2018;27(5):1111-20. e3.
  27. Grapov D, Fiehn O, Campbell C, Chandler CJ, Burnett DJ, Souza EC, et al. Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women. Physiological reports. 2020;8(17):e14547.
  28. Wang Y, Wagner KM, Morisseau C, Hammock BD. Inhibition of the soluble epoxide hydrolase as an analgesic strategy: A review of preclinical evidence. Journal of Pain Research. 2021:61-72.
  29. Sam MAK, Hidayatullah MF, Ekawati FF. The Effect of High Intensity Interval Training on Body Composition in Obesity Patients: Literature Review. European Journal of Sport Sciences. 2023;2(3):1-7.
  30. Khalafi M, Symonds ME. The impact of high intensity interval training on liver fat content in overweight or obese adults: A meta-analysis. Physiology & Behavior. 2021;236:113416.
  31. Leandro CG, Levada AC, Hirabara SM, MANHAS-DE-CASTRO R, De-Castro CB, Curi R, et al. Aprogram of moderate physical training for wistar rats based on maximal oxygen consumption. The Journal of Strength & Conditioning Research. 2007;21(3):751-6.
  32. Moin Norouzi, Abbas Sadeghi*, Mohaddeh Aksef Pakdehi, Qasim Torabi. 1400. The effect of eight weeks of intense intermittent exercise and caffeine consumption on the expression of glycogen synthase and the amount of liver glycogen in large diabetic rats. Medical Scholar 156. 41-55 [in persian].
  33. Nakamuta M, Fujino T, Yada R, Yada M, Yasutake K, Yoshimoto T, et al. Impact of cholesterol metabolism and the LXRα-SREBP-1c pathway on nonalcoholic fatty liver disease. International journal of molecular medicine. 2009;23(5):603-8.
  34. Yu Z, Feng Z, Fu L, Wang J, Li C, Zhu H, et al. Qingluotongbi formula regulates the LXRα-ERS-SREBP-1c pathway in hepatocytes to alleviate the liver injury caused by Tripterygium wilfordii Hook. f. Journal of Ethnopharmacology. 2022;287:114952.
  35. Szántó M, Gupte R, Kraus WL, Pacher P, Bai P. PARPs in lipid metabolism and related diseases. Progress in Lipid Research. 2021;84:101117.
  36. Vertegaal AC. Signalling mechanisms and cellular functions of SUMO. Nature Reviews Molecular Cell Biology. 2022;23(11):715-31.
  37. Chenfei Z, Haizhen Y, Jie X, Na Z, Bo X. Effects of aerobic exercise on hippocampal SUMOylation in APP/PS1 transgenic mice. Neuroscience Letters. 2022 Jan 10;767:136303.https://doi.org/10.1016/j.neulet.2021.136303
  38. Gehlert S, Klinz FJ, Willkomm L, Schiffer T, Suhr F, Bloch W. Intense resistance exercise promotes the acute and transient nuclear translocation of small ubiquitin-related modifier (SUMO)-1 in human myofibres. International Journal of Molecular Sciences. 2016 Apr 29;17(5):646.https://doi.org/10.3390/ijms17050646
  39. London E, Stratakis CA. The regulation of PKA signaling in obesity and in the maintenance of metabolic health. Pharmacology & Therapeutics. 2022 Jan 17:108113. https://doi.org/10.1016/j.pharmthera.2022.108113
  40. Lynes MD, Leiria LO, Lundh M, Bartelt A, Shamsi F, Huang TL, et al. The cold-induced lipokine 12, 13-diHOME promotes fatty acid transport into brown adipose tissue. Nature medicine. 2017;23(5):631-7. https://doi.org/10.1038/nm.4297

 

 

 

Medical Journal of Mashhad university of Medical Sciences
Volume 66, Issue 3
September and October 2023
Pages 440-451