Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Plasmid Replicon Typing of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal Children Using PCR Method Based on Plasmid Incompatibility Groups

Document Type : Research Paper

Authors
zakiyeh imanpanah
Gholamreza Hashemitabar
Mahdi Askari Badouei
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
10.22038/mjms.2024.73904.4369
Abstract
Introduction: Plasmids play a the crucial role in the horizontal transfer of genes, especially antibiotic resistance and pathogenic agents, and not epidemiological studies have been conducted on the type of plasmids transmitted by Enteroaggregative Escherichia coli pathotypes. The main purpose of this study is to classify the plasmids of Enteroaggregative Escherichia coli isolates isolated from children with diarrhea based on the major incompatible plasmid groups according method of Johnsnon et al(2007).
Materials and Method: This descriptive study was performed on 44 isolates of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal to identify 18 plasmid replicons by The method of Johnsnon et al(2007).
Results: The results showed the FreP 35(79.54%) plasmid replicon was the most frequent and L/M plasmid replicon was the least frequent with 1(2.27%). IncFIC, IncN, IncW, Inc X, Inc HI1, Inc HI2 and IncFIIA replicons were not observed in any of the isolates.
Conclusion: The results of this study indicated a high diversity of profile plasmid incompatibility groups among the studied isolates.
Keywords
Plasmid
Replicon Typing
EAEC
Children's diarrhea
Multiplex-PCR
Subjects
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Medical Journal of Mashhad university of Medical Sciences
Volume 66, Issue 6
January and February 2024