Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Improvement of glycemic profile in response to high intensity interval training with emphasis on the change of RBP4 gene expression in hepatocytes of diabetic rats

Document Type : Research Paper

Authors
Zarrin parvizy Barjooei Raki 1
Abdolali Banaeifar 2
Sajad Arshadi 3
Mojtaba Eizadi 4
1 Department of Exercise Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Exercise Physiology, South Thran Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor of Exercise Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran
4 Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
10.22038/mjms.2023.73120.4335
Abstract
Introduction: Hepatic glucose release is one of the most important causes of hyperglycemia in type 2 diabetics. The present study was conducted with the aim of determining the effect of intense interval training on RBP4 gene expression of in liver hepatocytes, as well as blood glucose and insulin levels in type 2 diabetic (T2D) rats.
Methods: For this purpose T2D induced by intraperitoneal nicotinamide and STZ in 14 male Wistar rats (10 week old, 220 ± 10 g), then they were divided into control (n=7) and interval (n=7) groups. Rats in the interval group performed a 4-week interval training program (5 sessions per week) in the form of running on a treadmill. The control group did not participate in the exercise program. 48 hours after the last training session, RBP4 gene expression in hepatocytes as well as serum insulin and fasting glucose were compared between groups by independent t-test.
Results: Interval training led to a significant decrease in fasting glucose (p = 0.001) and RBP4 gene expression in liver cells (p = 0.031) compared to the control group. Also, serum insulin increased significantly in response to interval training compared to the control group (p = 0.001).
Conclusion: Based on these findings, the improvement of fasting glucose in response to interval training may be attributed to the reduction of RBP4 in liver hepatocytes. However, knowing the mechanisms responsible for the effect of exercise on glycemic profile requires more studies.
Keywords
Glycemic profile
Hepatic Gluconeogenesis
Gene expression
Type 2 diabetes
Subjects
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Medical Journal of Mashhad university of Medical Sciences
Volume 67, Issue 2
March and April 2024
Pages 621-629