Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Investigation of the effect and comparison of three types of exercise (muscular electrical stimulation, aerobic and resistance) on the expression of ACTN3 and ACE genes in overweight women

Document Type : Research Paper

Authors
Romina Molae 1
mahmoud soltani 2
Amir Rashidlamir 3
1 MSc, Faculty of Physical Mashhad Branch, Islamic Azad University, Mashhad
2 .Iran, Mashhad, University Azad Islamic, Branch Mashhad, Education Physical of Department, Professor Assistant
3 5. Department of Sport Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, I.R.Iran
10.22038/mjms.2024.77961.4519
Abstract
Introduction:
Nowadays, due to unhealthy lifestyle and diet and inactivity, people get involved in various diseases such as obesity, and overweight. The purpose of this study was to investigate and compare the effect of three types of exercise (muscular electrical stimulation, aerobic and resistance) on the expression of ACTN3 (Alpha-actinin-3) and ACE (Angiotensin-Converting Enzyme) genes in overweight women.
Methodology:
In this semi-experimental research, 40 overweight women were randomly divided into four groups of 10 people: 1) EMS training (electro-muscle stimulation), 2) endurance training, 3) resistance training, and 4) control. The subjects of the training groups performed the selected exercises related to their group for eight weeks. A blood sample was collected 48 hours before and after the training sessions to measure the expression of ACTN3 and ACE genes. Data were analyzed using correlated t-test, one-way ANOVA, and Tukey's post hoc test at a significance level of p<0.05.
Results:
The results showed that ACTN3 and ACE values increased significantly in the aerobic, resistance, and EMS groups after eight weeks of intervention. At the same time, there was no significant difference in any of these variables in the control group (P>0.05). Also, the results of one-way ANOVA showed that EMS training caused more changes in the expression of ACTN3 and ACE genes compared to other research protocols in overweight women (p<0.05).
Keywords
EMS
ACTN3
ACE
Resistance training
Endurance training
Subjects
  1. Khameneh NFAJEAAZ. Effects of four-week aerobic training, caloric restriction and vitamin D supplementation on serum BDNF levels and body composition in overweight and obese women. Daneshvar Medicine. 2022;30(1):12-26.
  2. Karkhaneh M, Taheri E, Qorbani M, Mohajeri Tehrani MR, Hoseini S. ASSESSMENT OF METABOLIC SYNDROME COMPONENTS IN OBESE WOMEN WITH NORMALWEIGHT COMPARED TO NON OBESE HEALTHY WOMEN. Iranian Journal of Diabetes and Lipid Disorders. 2015;14(4):279-86.
  3. almasi j, Mohamadbigi S, Zakery Pur F. The effect of eight weeks of intense circular exercise on serum irisin levels in inactive women. Physical Activity and Health. 2022;1(1):63-73.
  4. Aboubakri S, Ghara khanlou R, Molanouri Shamsi M. Evaluation of the Frequency of polymorphism rs1815739 (ACTN3) and rs8192678 (PPARGC1A) among Professional Male Soccer Players of Iranian Premier League: Case-Control Study. Journal of Sport Biosciences. 2021;13(2):213-26.
  5. Maffulli N, Margiotti K, Longo UG, Loppini M, Fazio VM, Denaro V. The genetics of sports injuries and athletic performance. Muscles, ligaments and tendons journal. 2013;3(3):173-89.
  6. Melián Ortiz A, Laguarta-Val S, Varillas-Delgado D. Muscle Work and Its Relationship with ACE and ACTN3 Polymorphisms Are Associated with the Improvement of Explosive Strength. Genes (Basel). 2021;12(8).
  7. Pickering C, Kiely J, Grgic J, Lucia A, Del Coso J. Can Genetic Testing Identify Talent for Sport? Genes (Basel). 2019;10(12).
  8. fallah a, fallahmohammadi z, behmanesh m, Gharakhanlou R, Alinaghizadeh M. The ACTN3 R577X polymorphism is associated with judo status in Iranian elite judo athletes. Journal of Applied Exercise Physiology. 2019;14(28):151-8.
  9. Fiorilli G, Quinzi F, Buonsenso A, Casazza G, Manni L, Parisi A, et al. A Single اجلاس of Whole-Body Electromyostimulation Increases Muscle Strength, Endurance and proNGF in Early Parkinson Patients. Int J Environ Res Public Health. 2021;18(10).
  10. Azadpour N, Tartibian B, Koşar ŞN. Effects of aerobic exercise training on ACE and ADRB2 gene expression, plasma angiotensin II level, and flow-mediated dilation: a study on obese postmenopausal women with prehypertension. Menopause. 2017;24(3):269-77.
  11. Mousavi SR, Batavani M, Ghofrani M. Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes. Journal title. 2021;29(4):93-102.
  12. Oji F, Yaghoubi H. The Association Study between D/I Polymorphism of Angiotensin-Converting Enzyme (ACE) Gene with Obesity in the Population of Ardabil Province. New Cellularand Molecular Biotechnology Journal. 2017;7(28):19-24.
  13. AA G, N K, R F, A R, G S, V A. Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats. Tehran University Medical Journal. 2013;71(1):37-45.
  14. Mohd Faridz Ahmad AHH. The Effects of Electrical Muscle Stimulation (EMS) towards Male Skeletal Muscle Mass. International Scholarly and Scientific Research & Innovation. 2015;9(12).
  15. Bellia A, Ruscello B, Bolognino R, Briotti G, Gabrielli PR, Silvestri A, et al. Whole-body Electromyostimulation plus Caloric Restriction in Metabolic Syndrome. Int J Sports Med. 2020;41(11):751-8.
  16. Teschler M, Wassermann A, Weissenfels A, Fröhlich M, Kohl M, Bebenek M, et al. Short time effect of a single اجلاس of intense whole-body electromyostimulation on energy expenditure. A contribution to fat reduction? Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 2018;43(5):528-30.
  17. Patel H, Alkhawam H, Madanieh R, Shah N, Kosmas CE, Vittorio TJ. Aerobic vs anaerobic exercise training effects on the cardiovascular system. World J Cardiol. 2017;9(2):134-8.
  18. Kraemer WJ, Ratamess NA. Fundamentals of resistance training: progression and exercise prescription. Med Sci Sports Exerc. 2004;36(4):674-88.
  19. Pickering C, Kiely J. ACTN3: More than Just a Gene for Speed. Front Physiol. 2017;8:1080.
  20. Alun Jones HEM, and David R. Woods Human Performance: A Role for the ACEGenotype? Exercise and Sport Sciences Reviews. 2002;30(4).
  21. Sheppard JM, Young WB. Agility literature review: classifications, training and testing. J Sports Sci. 2006;24(9):919-32.
  22. Morici G, Gruttad'Auria CI, Baiamonte P, Mazzuca E, Castrogiovanni A, Bonsignore MR. Endurance training: is it bad for you? Breathe (Sheff). 2016;12(2):140-7.
  23. Çamlı AA, Ak B, Arabacı R, Efe R. Recent Advances in Health Sciences: ST. KLIMENT OHRIDSKI UNIVERSITY PRESS 2016.
  24. . !!! INVALID CITATION !!! {}.
  25. Norman B, Esbjornsson M, Rundqvist H, Osterlund T, Glenmark B, Jansson E. ACTN3 genotype and modulation of skeletal muscle response to exercise in human subjects. J Appl Physiol (1985). 2014;116(9):1197-203.
  26. Kikuchi N, Nakazato K. Effective utilization of genetic information for athletes and coaches: focus on ACTN3 R577X polymorphism. J Exerc Nutrition Biochem. 2015;19(3):157-64.
  27. Houweling PJ, Papadimitriou ID, Seto JT, Perez LM, Coso JD, North KN, et al. Is evolutionary loss our gain? The role of ACTN3 p.Arg577Ter (R577X) genotype in athletic performance, ageing, and disease. Hum Mutat. 2018;39(12):1774-87.
  28. Bohannon RW. Considerations and Practical Options for Measuring Muscle Strength: A Narrative Review. Biomed Res Int. 2019;2019:8194537.
  29. Suchomel TJ, Nimphius S, Bellon CR, Stone MH. The Importance of Muscular Strength: Training Considerations. Sports Med. 2018;48(4):765-85.
  30. Rashidlamir A. Investigation of the Effect of Aerobic and Resistance Exercises on Peripheral Blood Mononuclear Cells ABCG1 Gene Expression in Female Athletes. The Journal of Shahid Sadoughi University of Medical Sciences. 2012;20(1):1-9.
  31. Taribiyan B, Baghaiee B, Hosseini SRA. Effect of eight week moderate exercise training on Angiotensin Converting Enzyme gene expression and Angiotensin II activity in middle-aged men. Razi Journal of Medical Sciences. 2012;19(102):53-64.
  32. The Effect of Eight Weeks Aerobic and Resistance Trainingon AMP-Activated Protein Kinase (AMPK) GeneExpression in Soleus Muscle and Insulin Resistance of STZ-Induced Diabetic Rat. journal of medical council of islamic republic of iran. 2019;37(2):81-7.
  33. Seyyed Reza Attarzadeh Hosseini MMR, Navideh Moien Neia. The effect of two different intensities resistance training on muscle growth regulatory myokines in sedentary young women. Arak Medical University Journal (AMUJ) 2016;19(112): 56-65.
  34. Mansour Salehi SMAAA. Investigation of ACTN-3 gene polymorphism in top Iranian athletes. Physical Education Research Institute of the Ministry of Science, Research and Technology. 2012;13:13-22.
  35. Eimari Eskandari R, Salehzadeh K, Shirmohamadzadeh M. The Relationship between Digit Ratio and Genotypes of Angiotensin Converting Enzyme with Strength Muscle on National Company South Oil Professional Weight Lifter Men. Sport Physiology. 2015;7(25):87-98.
  36. Mirzaei S, Siahkouhian M, Afroundeh R, khazani A, Anoshirvani S. Association of ACTN3 Gene Polymorphism with Muscle Strength and Power Indices in Adolescent Weightlifters in Ardabil. Journal of Applied Exercise Physiology. 2020;16(32):103-14.
  37. Amini S, Talebi N, Samadi A. Comparing the Effect of 8 Weeks Iso-distance Interval and Continuous Exercise Training on Appetite Control and Body Composition in Sedentary, Overweight and Obese Girls. Journal of Applied Health Studies in Sport Physiology. 2016;3(2):64-71.
Medical Journal of Mashhad university of Medical Sciences
Volume 67, Issue 2
March and April 2024
Pages 435-450