Antinociceptive effect of hydroalcoholic extract of Moringa (Moringa oleifera) in Male Rats

Khajeh, Hamide; Ganjali, Salehe; Rigi, Mahin; Ghafghazi, Leila; Mazarei, Ayoub

doi:10.22038/mjms.2024.82230.4736

Antinociceptive effect of hydroalcoholic extract of Moringa (Moringa oleifera) in Male Rats

Document Type : Research Paper

Authors

Hamide Khajeh ¹

Salehe Ganjali ²

Mahin Rigi ³

Leila Ghafghazi ⁴

Ayoub mazarei ⁵

¹ M. Sc. OF Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

² Assistant Professor Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran

³ Department of Aquatic Science, Hamon International Wetland Research Institute, Zabol Research Institute, Zabol , Iran

⁴ M. Sc. Graduate of Greenhouse Production Production, Department of Department of Horticultural Science. High School Secretary Zeinab, Zabul Education Management, Iran

⁵ PhD Graduate of Biotechnology, Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran

10.22038/mjms.2024.82230.4736

Abstract

Introduction
The application of herbal plants instead of synthetic drugs is increasing in recent years because of their lower side-effects and high varieties of efficient components. The aim of this study is to investigate some of the Chemical compounds and antinociceptive effects of hydroalcoholic extract of Moringa leaf on male rats.
Material and Method
In the present experimental study, 36 adult male rats were divided into 6 groups: control group, groups treated with the extract (100, 200 and 300 mg/kg), morphine (1 mg/kg) and naloxone (mg/kg) in combination with 300 mg/kg extract. The analgesic effects of HRC were assessed with writhing, tail-flick and formalin tests. The data were compared and investigated by One-wayANOVA and the Duncan test.
Results
According to the results, the highest amount of phenolic compounds, flavonoid and antioxidant activity was related to the concentration of 300 mg of extract.
Moringa oleifera leaf extract significantly inhibited the number of contractions induced by acetic acid (P<0.05, P<0.01 and P<0.01). Doses of 200 and 300 mg/kg HRC showed antinociceptive activity in the tail flick model (P<0.01). In formalin test, the highest effect was observed at dose of 300 mg/kg (P<0.01).
CONCLUSION: According to the present results, Moringa oleifera hydroalcoholic extracts have central and peripheral analgesic effects.

Keywords

Analgesic effect

Polyphenolic compounds

Medicinal plant

Hydroalcoholic Extract

Subjects