Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

The effect of aerobic training on insulin resistance and AMPK/PI3K axis in Soleus muscle of type 2 diabetic rats

Document Type : Research Paper

Authors
Hamid Eslami 1
Mojtaba Eizadi 2
Mania Rozbayani 3
1 PHD Candidate, Department of Exercise Physiology, Bo. C., Islamic Azad University, Borujerd, Iran
2 Department of Exercise Physiology, Sav. C., Islamic Azad University, Saveh, Iran.
3 Department of Exercise Physiology, Bo. C., Islamic Azad University, Borujerd, Iran
10.22038/mjms.2024.83168.4784
Abstract
Introduction: The role of genetic components in hypertrophy and glycemic profile especially in the presence of type 2 diabetes is well defined. This study aimed to determine the effect of aerobic training on AMPK/PI3K axis in Soleus muscle and insulin resistance in type 2 diabetic rats.
Methods: For this purpose, type 2 diabetic induced in fourteen male Wistar rats aged 10 week (220 ± 10 g) by intraperitoneal injection of nicotinamide and STZ and were randomly selected in exercise (n = 7) and control (n = 7) groups. The exercise rats participated in an aerobic training program (5 times weekly) in the form of running on the treadmill. 48 hours after the lasting exercise session, PI3K and AMPK expression in soleus muscle as well as fasting glucose were measured. Fasting glucose and insulin values were used to calculate insulin resistance. Data compared by independent t test between groups.
Results: Compared to control group, aerobic training resulted in significant increase in PI3K expression in soleus muscle (P = 0.005) and significant decrease in fasting glucose (P = 0.001) and insulin resistance (P = 0.010). AMPK expression did not change in response to aerobic training compare with control group (P = 0.238).
Conclusion: Based on these data, improved glucose and insulin resistance in response to aerobic training may be attributed to increased PI3K expression in soleus muscle. Understanding the mechanisms responsible for hypertrophy in response to exercise requires more studies in this area.
Keywords
Type 2 diabetes
Gene expression Aerobic training
Glycemic profile
Hypertrophy
Subjects
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Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 1
March and April 2025
Pages 32-40