تأثیر 6 هفته تمرین بر روی نوار گردان به همراه مصرف جنیستئین بر بیان ژن iNOS و Cdc2 در موش های بالب سی مبتلا به سرطان پستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران

2 گروه تربیت بدنی و علوم ورزشی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران (نویسنده مسئول)

چکیده

مقدمه: هدف پژوهش بررسی تاثیر 6 هفته تمرین بر روی نوار گردان به همراه مصرف جنیستئین بر بیان ژن iNOS و Cdc2 در موش های بالب سی مبتلا به سرطان پستان بود.
روش کار:  در این مطالعه بنیادی، تجربی که روی 40 سر موش ماده Balb/C انجام شد، بعد از القاء سرطان پستان، موش ها به صورت تصادفی به 4 گروه 10تایی موش های سرطانی، موش های سرطانی به همراه یک دوره تمرین روی نوارگردان، موش های سرطانی به همراه مصرف مکمل جنیستئین و موش های سرطانی به همراه یک دوره تمرین روی نوارگردان و مصرف مکمل جنیستئین تقسیم شدند. گاواژ مکمل خوراکی جنیستئین بین 100 الی 150 میکرولیتر، روزانه 5 روز در هفته، به مدت شش هفته اجراء گردید. تمرین روی نوارگردان با سرعت 20 متر در دقیقه، شیب صفر درصد به مدت 30 دقیقه در روز، 5 بار در هفته به مدت 6 هفته اجراء گردید. جهت تجزیه و تحلیل داده ها از آنالیز واریانس دوطرفه با اندازه گیری های مکرر استفاده شد.
نتایج: نتایج نشان داد اثر اصلی تمرین روی نوارگردان و اثر اصلی مصرف مکمل جنیستئین بر بیان ژن­ها در موش­های ماده Balb/C بعد از القاء سرطان پستان، از نظر آماری معنی­دار می­باشد (05/0 > P).
نتیجه گیری: فعالیت ورزشی و مصرف جنیستئین از طریق کاهش بیان ژن  iNOS و CDC-2 در موش های بالب سی القا شده با سرطان پستان، عملا از پیشرفت سرطان جلوگیری می کند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of 6 weeks of treadmill training combined with genistein consumption on iNOS and Cdc2 gene expression in BalbC mice with breast cancer

نویسندگان [English]

  • Seyadeh Zahra Hajiaghaei 1
  • Sanaz Mirzayan Shanjani 2
  • Zohreh Afsharmand 1
  • Yaser Kazemzadeh 1
1 Department of Exercsie Physiology, Isl.C., Islamic Azad University, Islamshahr, Iran
2 Department of Exercsie Physiology, Isl.C., Islamic Azad University, Islamshahr, Iran (Corresponding Author)
چکیده [English]

Introduction: The aim of the study was to investigate the effect of 6 weeks of treadmill exercise combined with genistein on iNOS and Cdc2 gene expression in Balb/C mice with breast cancer.
 Methods: In this basic, experimental study, which was conducted on 40 female Balb/C mice, after induction of breast cancer, the mice were randomly divided into 4 groups of 10: cancer mice, cancer mice with a treadmill exercise period, cancer mice with genistein supplementation, and cancer mice with a treadmill exercise period and genistein supplementation. Oral gavage of genistein supplement between 100 and 150 microliters was performed, 5 days a week, for six weeks. Treadmill exercise was performed at a speed of 20 meters per minute, 0% incline for 30 minutes per day, 5 times a week for 6 weeks. Two-way analysis of variance with repeated measures was used to analyze the data.
Results: The results showed that the main effect of treadmill exercise and the main effect of genistein supplementation on gene expression in female Balb/C mice after induction of breast cancer were statistically significant (P < 0.05).
Conclusion: Exercise and genistein intake effectively prevent cancer progression by reducing iNOS and CDC-2 gene expression in Balb/C mice induced with breast cancer.

کلیدواژه‌ها [English]

  • Aerobic training
  • genistein supplementation
  • iNOS
  • Cdc2
  • Balb/c mice
  • breast cancer

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 68، شماره 5
آذر-دی1404
آذر و دی 1404
صفحه 1595-1603

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