Evaluation the cytotoxic and apoptotic effects of Air and Oxygen Nanobubbles on Liver Cancer Cells (HUH7)

Document Type : Research Paper


1 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran.

2 Department of Technology and Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Neurologic inflammatory research center, Mashhad University of Medical Sciences, Mashhad, Iran.


Introduction: Hepatocellular carcinoma (HCC), has been considered as a prevalent type of cancer, affects million people globally each year. Nanotechnology, a progressive field of technology in recent years, has impressively developed in agriculture, industry, and healthcare systems. In the fields of pharmacy and medicine, multifunctional nanoparticles are designed for various purposes, especially the target therapy, the current therapeutic strategy in treatments of HCC.
The aim of the present study was the determination of the cytotoxic effects of micro nano bubbles on the human liver cancer cell line (Huh7-7).
Materials and Methods: The cytotoxicity effects of air and oxygen nano-micro bubbles on HUH7 cells which were treated with different concentrations of nano micro bubbles (1.95 – 500 µl/ml) were determined by calorimetric MTT assay. Histological changes have been checked and reported.
Results: Our results show that the nano micro bubbles inhibits the Huh7 cells proliferation by dose-dependent manner after 48 hours treatment (IC50 = 158.60 µl/ml).
Conclusion: On the basis of obtained data, Oxygen nano micro bubbles induced the apoptosis in Huh7 cells in the certain concentration. Nevertheless, nano micro bubbles may light on the new therapeutic strategy in treatment of hepatocellular carcinoma.   


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