Evaluation Resistance prevalence of Staphylococcus Aureus β-Lactamase from Nasal of Emergency Staff in Ghaem Hospital of Mashhad 1397

Document Type : Research Paper


1 Medical laboratory sciences department-Paramedical sciences school- Medical sciences university-Mashhad-Iran.

2 Molecular Cell Bachelor, Neyshabour Azad University Iran.

3 Bachelor of Science in Laboratory, para medical School of Mashhad.


Background: Staphylococcus aureus is a Gram-positive cocci which is one of the main infection causes. 20-30% of healthy people carry staphylococcus in their nasal cavities. Staphylococci resistant to beta-lactam is called MRSA. Regarding the presence of MRSA in the therapeutic environment. The sample was taken by swapping from nose secretion of emergency department staff at Ghaem Hospital. This study aims staphylococcus resistant to antibiotics.
Methods: In this study, 100 emergency medical personnel of Ghaem Hospital in Mashhad were sampled from nasal secretions from Farvrdin to Aban 1397. Samples were taken by two sterile wet swabs from nasal secretions and by chemical test, staph coccus was detected and antibiotic susceptibility test was done by Kirby Bayer method.
Results: From a total of 100 employees in emergency departments of Ghaem Hospital, 68 samples revealed Staphylococcus aureus (68%), while 21 samples of Staphylococcus aureus were found to be beta-lactamase positive. Resistance to antibiotics was observed, 100% of samples were resistant to penicillin and the highest sensitivity to cefotaxime was 100%. It was notable that one sample was resistant to vancomycin (3.5%).
Conclusion: If an employee has MRSA in nose secretion, they shouldn’t have direct contact with patients. More caution is needed in hospital occupations.


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