Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Investigation of the K1 and K2 capsular serotypes prevalence of Klebsiella pneumonia isolated from Urinary tracts and wounds infections

Document Type : Research Paper

Authors
Fatemeh Keshavarzi 1
Fariba Lahoorpour 2
Rezvan Doustipour 3
1 Associate Professor of Molecular Genetics, Department of Biology, Sanandaj branch, Islamic Azad University, Sanandaj, Iran
2 Assistant Professor of Bacteriology, Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran
3 MSc of Microbiology, Department of Biology, Sanandaj branch, Islamic Azad University, Sanandaj, Iran
10.22038/mjms.2020.16278
Abstract
Abstract
Introduction: Klebsiella pneumonia, a gram-negative bacterium, is one of the main causes of nosocomial infections. The purpose of this study is to determine K1 and K2 capsule serotypes in samples collected from hospitals in Kermanshah.
Materials & methods: This study was a Cross-Sectional – Descriptive research. The Samples were collected of patients with urinary tract infections or wounds caused by burns between the first 4 months of 2016. The serotype of samples was determined by using of PCR method for wzy and magA genes belong to CPS cluster which are necessary for biosynthesis of polysaccharide capsule K1 and K2, respectively, after approving bacteria by phenotypic method. 
Results: The findings showed that out of 60 samples approved in phenotypic studies with a 1: 1 ratio of urine and wound samples, 37 samples (61.67%) was belonged to K1 and 23 samples (38.33%) to K2 serotype. Also, among the urine samples of patients, 22 samples (73.33%) had K1 serotype and about of the samples belonging to wounds caused by burns, 21(70%) and 9(30%) samples had K1 and K2 serotype, respectively.
Keywords
Klebsiella pneumonia
Colony PCR
magA
wzy
  1. Vali L, Dashti AA, Jadaon MM, El-Shazly S. The emergence of plasmid mediated quinolone resistance qnrA2
    in extended spectrum β-lactamase producing Klebsiella pneumoniae in the Middle East. DARU Journal of
    Pharmaceutical Sciences. 2015 Dec 1;23(1):34.
    2. Turton JF, Englender H, Gabriel SN, Turton SE, Kaufmann ME, Pitt TL. Genetically similar isolates of
    Klebsiella pneumoniae serotype K1 causing liver abscesses in three continents. Journal of medical
    microbiology. 2007 May 1;56(5):593-7.
    3. Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, Grimont P. Virulent clones of
    Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic
    characterization. PloS one. 2009 Mar 25;4(3):e4982.
    4. Lau HY, Clegg S, Moore TA. Identification of Klebsiella pneumoniae genes uniquely expressed in a strain
    virulent using a murine model of bacterial pneumonia. Microbial pathogenesis. 2007 Apr 1;42(4):148-55.
    5. Diancourt L, Passet V, Verhoef J, Grimont PA, Brisse S. Multilocus sequence typing of Klebsiella pneumoniae
    nosocomial isolates. Journal of clinical microbiology. 2005 Aug 1;43(8):4178-82.
    6. Pan YJ, Lin TL, Chen YH, Hsu CR, Hsieh PF, Wu MC, Wang JT. Capsular types of Klebsiella pneumoniae
    revisited by wzc sequencing. PloS one. 2013 Dec 9;8(12):e80670.
    7. Yeh KM, Lin JC, Yin FY, Fung CP, Hung HC, Siu LK, Chang FY. Revisiting the importance of virulence
    determinant magA and its surrounding genes in Klebsiella pneumoniae causing pyogenic liver abscesses: exact
    role in serotype K1 capsule formation. The Journal of infectious diseases. 2010 Apr 15;201(8):1259-67.
    8. Chuang YP, Fang CT, Lai SY, Chang SC, Wang JT. Genetic determinants of capsular serotype K1 of
    Klebsiella pneumoniae causing primary pyogenic liver abscess. The Journal of infectious diseases. 2006 Mar
    1;193(5):645-54.
    9. Cryz SJ, Mortimer PM, Mansfield V, Germanier R. Seroepidemiology of Klebsiella bacteremic isolates and
    implications for vaccine development. Journal of Clinical Microbiology. 1986 Apr 1;23(4):687-90.
    10. Fang CT, Chuang YP, Shun CT, Chang SC, Wang JT. A novel virulence gene in Klebsiella pneumoniae
    strains causing primary liver abscess and septic metastatic complications. The Journal of experimental
    medicine. 2004 Mar 1;199(5):697-705.
  2. 11. Ofek IT, Kabha KI, Athamna AB, Frankel G, Wozniak DJ, Hasty DL, Ohman DE. Genetic exchange of
    determinants for capsular polysaccharide biosynthesis between Klebsiella pneumoniae strains expressing
    serotypes K2 and K21a. Infection and immunity. 1993 Oct 1;61(10):4208-16.
    12. Kabha K, Nissimov L, Athamna A, Keisari Y, Parolis H, Parolis LA, Grue RM, Schlepper-Schafer J,
    Ezekowitz AR, Ohman DE. Relationships among capsular structure, phagocytosis, and mouse virulence in
    Klebsiella pneumoniae. Infection and immunity. 1995 Mar 1;63(3):847-52.
    13. Cortés G, Borrell N, de Astorza B, Gómez C, Sauleda J, Albertí S. Molecular analysis of the contribution of
    the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae
    in a murine model of pneumonia. Infection and immunity. 2002 May 1;70(5):2583-90.
    14. Izquierdo L, Coderch N, Piqué N, Bedini E, Corsaro MM, Merino S, Fresno S, Tomás JM, Regué M. The
    Klebsiella pneumoniae wabG gene: role in biosynthesis of the core lipopolysaccharide and virulence. Journal
    of bacteriology. 2003 Dec 15;185(24):7213-21.
    15. Lai YC, Peng HL, Chang HY. RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43,
    regulates K2 cps gene expression at the transcriptional level. Journal of bacteriology. 2003 Feb 1;185(3):788-
    800.
    16. Podschun R, Sievers D, Fischer A, Ullmann U. Serotypes, hemagglutinins, siderophore synthesis, and serum
    resistance of Klebsiella isolates causing human urinary tract infections. Journal of infectious diseases. 1993
    Dec 1;168(6):1415-21.
    17. Struve C, Bojer M, Nielsen EM, Hansen DS, Krogfelt KA. Investigation of the putative virulence gene magA
    in a worldwide collection of 495 Klebsiella isolates: magA is restricted to the gene cluster of Klebsiella
    pneumoniae capsule serotype K1. Journal of medical microbiology. 2005 Nov;54(Pt 11):1111-3.
    18. Yeh KM, Kurup A, Siu LK, Koh YL, Fung CP, Lin JC, Chen TL, Chang FY, Koh TH. Capsular serotype K1
    or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess
    in Singapore and Taiwan. Journal of clinical microbiology. 2007 Feb 1;45(2):466-71.
    19. Gierczynski R, Jagielski MA, Rastawicki WA, Kaluzewski S. Multiplex-PCR assay for identification of
    Klebsiella pneumoniae isolates carrying the cps loci for K1 and K2 capsule biosynthesis. Polish Journal of
    Microbiology. 2007;56(3):153.
    20. Feizabadi MM, Raji N, Delfani S. Identification of Klebsiella pneumoniae K1 and K2 capsular types by PCR
    and quellung test. Jundishapur Journal of Microbiology. 2013 Nov 1;6(9).
    21. Feizabadi MM, Raji N, Delfani S. Identification of Klebsiella pneumoniae K1 and K2 capsular types by PCR
    and quellung test. Jundishapur Journal of Microbiology. 2013 Nov 1;6(9).
    22. Onori R, Gaiarsa S, Comandatore F, Pongolini S, Brisse S, Colombo A, Cassani G, Marone P, Grossi P,
    Minoja G, Bandi C. Tracking nosocomial Klebsiella pneumoniae infections and outbreaks by whole-genome
    analysis: small-scale Italian scenario within a single hospital. Journal of clinical microbiology. 2015 Sep
    1;53(9):2861-8.
    23. Khamesipour F, Tajbakhsh E. Analyzed the genotypic and phenotypic antibiotic resistance patterns of
    Klebsiella pneumoniae isolated from clinical samples in Iran.
    24. Rahn A, Drummelsmith J, Whitfield C. Conserved organization in the cps gene clusters for expression of
    Escherichia coli group 1 K antigens: relationship to the colanic acid biosynthesis locus and the cps genes from
    Klebsiella pneumoniae. Journal of bacteriology. 1999 Apr 1;181(7):2307-13.
    25. Nypaver CM, Thornton MM, Yin SM, Bracho DO, Nelson PW, Jones AE, Bortz DM, Younger JG. Dynamics
    of Human Complement–Mediated Killing of Klebsiella pneumoniae. American journal of respiratory cell and
    molecular biology. 2010 Nov;43(5):585-90.
    26.
    . Turton JF, Baklan H, Siu LK, Kaufmann ME, Pitt TL. Evaluation of a multiplex PCR for detection of
    serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes. FEMS
    microbiology letters. 2008 Jul 1;284(2):247-52.
Medical Journal of Mashhad university of Medical Sciences
Volume 63, Issue 2
July and August 2020
Pages 2427-2435