Effect of High Intensity Interval Training and Continous Training on the Gene Expression of Bcl2, Bax and P-53 Protein in the Left Ventricle type 2 diabetes of Male Rats

Document Type : Research Paper

Authors

1 Department of Exercise physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

2 2- Professor, Department of exercise physiology, Faculty of physical education and sport science, Islamic Azad University, Central Tehran Branch, Tehran, Iran.

3 Associate professor , Department of exercise physiology, Faculty of physical education and sport science, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

Abstract

Abstract
Background: The purpose of this study was the Effect of High-Intensity Interval Training and Continous Training on the Gene Expression of Bcl2, Bax and  P-53 Protein in the Left Ventricle type 2 diabetes of Male Rats
Material and Methods: 24 male diabetic rats were divided into 3 groups of 8; High-intensity intensity interval training (HIIT), Moderate training (CT), and Control (C). Diabetes was induced in a pellet with a high-fat diet (30% fat and 25% fructose) for 24 weeks. Glucose oxidase was used to measure glucose in plasma using and the HOMA-IR method was used to measure insulin resistance index. PCR-Real time was used to determine the expression of BAX and BCL-2 genes and P-53 Protein Synthesis.
Results: geneexperision of Bcl-2 was significantly increased in the HIIT group compared to the CT (P=0/002) and control groups (p=0/000) and in the CT group compared to the Control group (p=0/033). Bax gene was significantly decreased in both groups HIIT(p=0/000) and CT(p=0/009) compared to the Control group (P=0/0001). P-53 protein synthesis was significantly in HIIT compared to CT (p=0/012) and control group (p=0/000).
Conclusion: Based on the findings, 8 weeks of intensity interval training with a higher effect on increasing Bcl-2 gene expression and decreased P-53 protein synthesis may improve left ventricular apoptosis in diabetic rats which reduces fibrosis and dysfunction in the heart tissue and may be an effective factor in reducing diabetic cardiomyopathy.

Keywords


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