Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Effect of High Intensity Interval Training and Continous Training on the Gene Expression of Bcl2, Bax and P-53 Protein in the Left Ventricle type 2 diabetes of Male Rats

Document Type : Research Paper

Authors
Alireza Safar nezhad 1
Maghsoud Peeri 2
Hasan Matin Homaee 3
1 Department of Exercise physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 2- Professor, Department of exercise physiology, Faculty of physical education and sport science, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
3 Associate professor , Department of exercise physiology, Faculty of physical education and sport science, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
10.22038/mjms.2021.17582
Abstract
Abstract
Background: The purpose of this study was the Effect of High-Intensity Interval Training and Continous Training on the Gene Expression of Bcl2, Bax and  P-53 Protein in the Left Ventricle type 2 diabetes of Male Rats
Material and Methods: 24 male diabetic rats were divided into 3 groups of 8; High-intensity intensity interval training (HIIT), Moderate training (CT), and Control (C). Diabetes was induced in a pellet with a high-fat diet (30% fat and 25% fructose) for 24 weeks. Glucose oxidase was used to measure glucose in plasma using and the HOMA-IR method was used to measure insulin resistance index. PCR-Real time was used to determine the expression of BAX and BCL-2 genes and P-53 Protein Synthesis.
Results: geneexperision of Bcl-2 was significantly increased in the HIIT group compared to the CT (P=0/002) and control groups (p=0/000) and in the CT group compared to the Control group (p=0/033). Bax gene was significantly decreased in both groups HIIT(p=0/000) and CT(p=0/009) compared to the Control group (P=0/0001). P-53 protein synthesis was significantly in HIIT compared to CT (p=0/012) and control group (p=0/000).
Conclusion: Based on the findings, 8 weeks of intensity interval training with a higher effect on increasing Bcl-2 gene expression and decreased P-53 protein synthesis may improve left ventricular apoptosis in diabetic rats which reduces fibrosis and dysfunction in the heart tissue and may be an effective factor in reducing diabetic cardiomyopathy.
Keywords
Cardiomyocyte
Apoptosis
BAX
BCL2
P-53
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Medical Journal of Mashhad university of Medical Sciences
Volume 63, Issue 5
January and February 2021
Pages 2769-2881