Evaluation of vitamin D levels using spermogram selection

Document Type : Research Paper


1 Assistant Professor, Department of Urology, Mashhad University of Medical Sciences, Mashhad, Iran

2 Assistant Professor of Community Medicine, Clinical Research Development Unit, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

3 General Physician of Mashhad University of Medical Sciences


Introduction: Despite the high costs of infertility treatment and the lack of access to advanced treatment for many couples, the role of infertility in the occurrence of psychological and social problems can also be mentioned.
Considering the possible effects of vitamin D on the characteristics of sperm, a purposeful relationship between these two categories can be of great help to the community health system. The aim of this study was to evaluate the level of vitamin D in patients with spermogram disorder and compare it with the control group.
Methods: This descriptive-analytical study was performed on 114 patients were divided into two groups of 57 controls and infertile in the age range of 20 to 59 years and the infertile group were men with fertility disorders.The control group included fertile men with at least one child under the age of 5. Spermograms and levels of vitamin-D and hormonal factors such as LH and FSH, testosterone in both control and infertile groups were evaluated and comparisons were made between the two groups.
Results: Sperm count parameters and sperm motility and sperm shape Vitamin D were a significant difference between the two groups (P <0.001). It was also found that vitamin D had a significant and direct correlation with testosterone in the patient group (P = 0.001).
Conclusion: These results suggest the possible relationship between vitamin D and sperm quality in men. Therefore, treatment of vitamin D deficiency in men can be a solution to reduce infertility disorder in men.


1. Makar RS, Toth TL. The evaluation of
infertility. Pathology Patterns Reviews. 2002
Jun 1;117(suppl_1):S95-103.
2. Jodar M, Soler-Ventura A, Oliva R, of
Reproduction MB, Development Research
Group. Semen proteomics and male infertility.
Journal of proteomics. 2017 Jun 6;162:125-
3. Bracke A, Peeters K, Punjabi U, Hoogewijs
D, Dewilde S. A search for molecular
mechanisms underlying male idiopathic infertility. Reproductive biomedicine online.
2018 Mar 1;36(3):327-39.
4. Irani M, Mirzaei K, Maleki N, Entezari E. The
role of vitamin D in male and female
reproductive health: a review study. The
Iranian Journal of Obstetrics, Gynecology and
Infertility. 2017 May 22;20(3):98-109.
5. Ciccone IM, Costa EM, Pariz JR, Teixeira
TA, Drevet JR, Gharagozloo P, Aitken RJ,
Hallak J. Serum vitamin D content is
associated with semen parameters and serum
testosterone levels in men. Asian journal of
andrology. 2021 Jan;23(1):52.
6. Mahdiani E, Khadem Haghighian H, Javadi
M, Karami AA, Kavianpour M. Effect of
Carob (Ceratonia siliqua L.) oral
supplementation on changes of semen
parameters, oxidative stress, inflammatory
biomarkers and reproductive hormones in
infertile men. Sci J Kurdistan Univ Med Sci.
2018 Jan 1;23:56-66.
7. Arab D, Doustmohammadi H, Ardestani
Zadeh A. Dietary supplements in the
management of varicocele‐induced infertility:
A review of potential mechanisms.
Andrologia. 2021 Feb;53(1):e13879.
8. Jensen MB. Vitamin D and male
reproduction. Nature Reviews Endocrinology.
2014 Mar;10(3):175.
9. Azizi E, Naji M, Shabani-Nashtaei M,
Aligholi A, Najafi A, Amidi F. Association of
serum content of 25-hydroxy vitamin D with
semen quality in normozoospermic and
oligoasthenoteratozoospermic men.
International Journal of Reproductive
BioMedicine. 2018 Nov;16(11):689.
10. Babu SR, Sadhnani MD, Swarna M,
Padmavathi P, Reddy PP. Evaluation of FSH,
LH and testosterone levels in different
subgroups of infertile males. Indian Journal of
Clinical Biochemistry. 2004 Jan 1;19(1):45-9.
11. Blomberg Jensen M, Bjerrum PJ, Jessen TE,
Nielsen JE, Joensen UN, Olesen IA, Petersen
JH, Juul A, Dissing S, Jørgensen N. Vitamin
D is positively associated with sperm motility
and increases intracellular calcium in human
spermatozoa. Human reproduction. 2011 Jun
12. Hammoud AO, Meikle AW, Peterson CM,
Stanford J, Gibson M, Carrell DT. Association
of 25-hydroxy-vitamin D levels with semen
and hormonal parameters. Asian journal of
andrology. 2012 Nov;14(6):855.
13. Fields J, Trivedi NJ, Horton E, Mechanick JI.
Vitamin D in the Persian Gulf: integrative
physiology and socioeconomic factors.
Current osteoporosis reports. 2011
14. Abbasihormozi S, Kouhkan A, Alizadeh AR,
Shahverdi AH, Nasr‐Esfahani MH, Sadighi
Gilani MA, Salman Yazdi R, Matinibehzad A,
Zolfaghari Z. Association of vitamin D status
with semen quality and reproductive
hormones in Iranian subfertile men.
Andrology. 2017 Jan;5(1):113-8.
15. Waud K, Bocca S. Role of vitamin D
deficiency in male fertility. Fertility and
Sterility. 2015 Feb 1;103(2):e39.
16. Akhavizadegan H, Karbakhsh M. Comparison
of serum vitamin D between fertile and
infertile men in a vitamin D deficient endemic
area: a case-control study. Urologia Journal.
2017 Oct;84(4):218-20.