Comparison of the Effect of Two types of High-Intensity Interval Training on the Expression Genes of IRS-1 and IRE-1α in the Cardiomyocytes type 2 diabetes of Obes Male Rats


1 Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Professor, Department of exercise physiology, Faculty of physical education and sport science, Islamic Azad University, Central Tehran Branch, Tehran, Iran.

3 Department of exercise physiology, Alzahra University Sport Sciences, Tehran, Iran


Background: The purpose of this study was Comparison Comparison the Effect of Two types of High-Intensity Interval Training on the Expression Genes of IRS-1 and IRE-1α in the Cardiomyocytes type 2 diabetes of Obes Male Rats.
Material and Methods: 36 male Wistar rats were divided into 4 groups: type1 high-intensity interval training (HIIT1:1), type2 high-intensity interval training (HIIT2:1), diabetic control (DC), and nondiabetic control (NC). Plasma glucose concentration was measured by the glucose oxidase method. ELISA method was used to measure the insulin levels and the HOMA-IR method was used to measure insulin resistance index. A real-time PCR technique was used to evaluate the gene expression of the IRS-1 and IRE-1α genes. Results were analyzed with one-way ANOVA and Tukey post hoc tests at the alpha level of 0/05.
Results: gene expression of IRS-1 in both groups of HIIT2:1(p= 0.002) and HIIT1:1(p= 0.006) showed a significant decrease compared to the DC group and in HIIT2:1(P=0/043) showed a significant decrease compared to the HIIT1:1 group. a gene of IRE-1α in both groups of HIIT2:1(p= 0.005) and HIIT1:1(p= 0.007) showed a significant decrease compared to the DC group and in HIIT2:1(P=0/001) showed a significant decrease compared to the HIIT1:1 group. The mean weight values and insulin resistance index in the HIIT1:1 group and glucose were significantly lower in the HIIT2: 1 group.
Conclusion: High-intensity interval training type 2 with a higher effect on decreasing IRS-1 and IRE-1α genes expression in cardiomyocytes of obese diabetic rats may it can improve gene dysregulation.


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