The Effect of Aerobic Maximal Workload exercises on galactin-3, plasma creatine kinase MB and lactate dehydrogenase concentration of Iranian Soccer premier league players

Document Type : Research Paper


1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 Associate Professor, Department of Physical Education and Sports Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. (Corresponding Author)

3 Associate Professor, Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, Central Tehran Branch, Islamic Azad University, Iran.

4 Assistant Professor, Department of Physical Education and Sports Science, Science and Research Branch, Islamic Azad University, Tehran, Iran


Introduction: Cardiac biomarkers play an important role in predicting heart damage. The aim of this study was to investigate The effect of aerobic maximal workload exercises on Galactin-3, plasma Creatine Cinase-MB and lactate dehydrogenase concentration of Iranian Soccer premier league players.
Methods: The statistical population consisted of players of the Iranian Football Premier League in the season 1399-1400. 20 football players were selected as available samples. Evaluation of aerobic activity was incremental to maximal with the implementation of the Bruce protocol. Blood samples were taken before, immediately and 24 hours after the end of the protocol. To test the hypotheses and compare the dependent variables in different groups, analysis of variance in repeated measures and Bonferroni test were used to compare between blood sampling steps. Data analysis was performed using SPSS25 software.
Results: There was no significant difference in the amount of Galactin-3 between the pre-test and post-test stages and also between the post-test and 24 hours after the test. There was a significant difference in Creatine Kinase-MB and Lactate Dehydrogenase levels between pre-test and post-test stages as well as between pre-test and 24 hours after test.
Conclusion: The merobic maximal workload exercises alters serum Galactin-3 levels in Premier League players, possibly due to inflammatory and anti-inflammatory effects during exercise and post-exercise recovery. Also, based on the findings of the present study, increasing aerobic exercise to the maximum caused a significant difference in levels of creatine kinase MB and lactate dehydrogenase in the post-test and 24 hours after the test.


1. Lee IM, Manson JE, Hennekens CH, Paffenbarger RS, Jr, Body weight and mortality. 1993. A 27-year followup of middle-aged men. JAMA;270:2823-8.
2. Rifai N, Douglas PS, OToole M , Rimm E, Ginsburg GS, 1999. Cardiac troponin T and I echocardiographic(
correction of electrocardiographic) wall motion analyses and ejection fractions in athletes participating in the
Hawaii Ironman Triathlon. Am J Cardiol;83:1085-9.
3. Shave RE, Whyte GP, George K , Gaze DC, Collinson PO. 2005. Prolonged exercise should be considered
alongside typical symptoms of acute myocardial infarction when evaluation increases in cardiac troponin T.
4. Neilan TG, Januzzi JL, Lee-Lewandrowski E, et al. 2006. Myocardial injury and ventricular dysfunction related
to training levels among nonelite participants in the Boston marathon. Circulation;114:2325-33.
5. Middleton N, George K, Whyte G, Gaze D, Collinson P, Shave R. 2008. Cardiac troponin T release is stimulated
by endurance exercise in healthy humans. J Am Coll Cardiol a;52:1813-4.
6. Felker GM.Fiuzat M, Shaw LK, Clare R, Whellan DJ, Bettari L, et al. 2012. Galectin-3 in Ambulatory Pations
with Heart Failure: Results from the HF-Action Study. Circ Heart Fail,; 5; 72-8.
7. Braunwald E, 2008. Biomarkers in Heart Failure. N Engl J Med.; 358: 2148-59.
8. 8.Mudaraddi, Rakesh & Kulkarni, Shrirang & Trivedi, Dhiraj & Patil, Vidya & Kamble, Pramod. 2015.
Association of Serum Electrolytes and Urea Levels with Cardiac Markers in Acute Myocardial Infarction.
International Journal of Clinical Biochemistry and Research. 2. 233. 10.5958/2394-6377.2015.00009.X.
9. Hattassch R, Spethmann S, de Boer RA, Ruifrok WP, Schattke S, Wagner M, et al. 2013. Galectin-3 increase in
endurance athletes. Europ J prevent cardiol, 21: 1192-1199.
10. Kim H, Lee J, Hyun JW, Park JW, Joo HG and Shin T. 2007. Expression and immunohistochemical localization
of galectin-3 in various mouse tissues. Cell Biol Int, 31: 655-662.
11. Berent R, von Duvillard SP, Crouse SF, Auer J, Green JS, Sinzinger H and Schmid P. 2009. Short-term
residential cardiac rehabilitation reduces B- type natriuretic peptide. Europ J Cardiovascular Preven Rehab, 16:
12. Sharma UC, Pokharel S, Van Brakel TJ, Van Berlo JH, Cleutjens JP, Schroen B, et al. 2004. Galectin-3 marks
activated macrophages in failure- prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation,
110: 3121-3128.
13. Detain D, Messika- Zeitoun D, Chen HH, Rossi A, Avierinos JF, Scott C. et al. 2006. Association of B-type
natriuretic peptide activation to left ventricular end-systolic remodeling in organic and functional mitral
regurgitation. Am J Cardiol, 97: 1029-34.
14. Eggers KM, Jernberg T, Lindahl B. 2019. Cardiac troponin elevation in patients without a specific diagnosis. J
Am Coll Cardiol.;73:1–9. doi: 10.1016/j.jacc.2018.09.082.
15. Roos A, Bandstein N, Lundbäck M, Hammarsten O, Ljung R, Holzmann MJ. 2017. Stable high-sensitivity
cardiac troponin T levels and outcomes in patients with chest pain. J Am Coll Cardiol.;70:2226–2236.
16. Thygesen K, Mair J, Katus H, Plebani M, Venge P, Collinson P, Lindahl B, Giannitsis E, Hasin Y, Koenig W,
Mueller C, Huber K, Hamm C, Jaffe A. 2010. Recommendation for The use of Cardiac Troponin Measurement
in Act Cardiac Cure. European Heart Journal 31, 2197-2206
17. Shave R, Baggish A, George K, Wood M, Scharhag J, Whyte G, Gaze D, Thompson PD. 2010. Exercise –
induced Cardiac Troponin Elevation: Evidence, Mechanisms, and Implication. J Am Coll Cardiol a; 56: 169 –
18. Shave R, George KP,Atkinson G,Hart E,Middleton N,Whyte G, etal. 2007. Exercise-induced cardiac troponinT
release:a meta-analysis.Med Sci Sports Exerc;39:2099–106.
19. Lin, Y.-H., Lin, L.-Y., Wu, Y.-W., Chien, K.-L., Lee, C.-M., Hsu, R.-B., . . . Liao, L.-C. 2009. The relationship
between serum galectin-3 and serum markers of cardiac extracellular matrix turnover in heart failure patients.
Clinica chimica acta, 409(1-2), 96-99.
20. Lippi, G., Cervellin, G., Banfi, G., & Plebani, M. 2011. Cardiac troponins and physical exercise. It’s time to
make a point. Biochemia medica: Biochemia medica, 21(1), 55-62.