Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Effect of High Intensity Interval Training and Thyme Honey on Hepatic miR-423-5P, FAM3A, Akt2 Pathway of Type ll Diabetic rats

Document Type : Research Paper

Authors
marjan abdi ardekani 1
Abdol Ali Banaei Far 2
Sajjad Arshadi 2
Hossein Abednatanzi 3
1 Teacher of district one of education in Shiraz
2 Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, Islamic Azad University, South Tehran Branch, Tehran. Iran.
3 Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
10.22038/mjms.2022.58858.3440
Abstract
Introduction: Micro-RNAs are effective in inflicting diabetes type II and its side effects. The Purpose of this study was to evaluate the effect of High Intensity Interval Training & thyme honey on expression of the genes of the hepatic miR-423-5P - FAM3A-AKt2 pathway in rats.
Materials and Methods: 36 male rats were fed with high-fat diets for 20weeks and were then injected streptozotocin (25 mg/kg) in order to inflict diabetes type II. The 36rats were then divided into four groups: Control, regular exercise group, nutritional intervention group and exercise with nutritional intervention group. Regular exercise included five sessions a week for eight weeks and nutritional intervention was done at a dosage of 3 g/kg of thyme honey using a gauge method. The amount of expression of miR-423-5P, mRNA FAM3A and mRNA AKt2 genes of the liver were assessed using RT-PCR and evaluation kits. The obtained findings were analyzed using the two-way analysis of variance and Bonferroni post hoc test.
Results: Expression of the miR-423-5P gene was significantly lower in all groups compared to control, expression of mRNA FAM3 had insignificant increase and expression of mRNA AKt2 had a significant increase in groups of regular exercise and exercise with nutritional intervention. This gene had an insignificant increase of expression in the group with only nutritional intervention. (p value<0.05)
Discussion: It seems that HIIT & consumption of thyme honey, both alone and interactively with greater effectiveness, can change in expression of the mentioned gene in liver of rats with diabetes type II.
Keywords
Diabetes Type II
miR-423-5P - FAM3A -AKt2
HIIT
Thyme Honey
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Medical Journal of Mashhad university of Medical Sciences
Volume 65, Issue 1
May and June 2022
Pages 101-115