Investigating the apoptotic Effect of Oxygen, Ozone and Air Nanomicrobubbles on Melanoma Cancer

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran

2 Department of Medical Genetics and Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Introduction: Skin melanoma is one of the most invasive types of skin cancer. It accounts for about 75% of all skin cancer deaths and has not yet been definitively treated. As a result, early diagnosis and prevention of the disease is important. Much research has been done to find ways to treat and cure cancer. One of the newest methods in this field is the use of nanotechnology to detect and inhibit cancer cells. In this study, the effect of using Nano microbubbles of Air, Oxygen and Ozone on A375 melanoma cancer cell line was evaluated.
Materials and Methods: A375 cell line treated with different concentrations of Nano microbubbles of Air, Oxygen and Ozone and were evaluated by MTT method. In order to measure cell viability, MTT plate was added to each well and then the plate was incubated.
Results: According to the results of this study, a decrease in the viability of A375 cancer cells with an increase in the concentration of Oxygen and Ozone Nano microbubbles after 24 hours indicates apoptosis and has a significant inhibition of the growth of these cells.
Conclusion: The results of this study indicate that Nano microbubbles of Oxygen and Ozone in high concentrations cause cell death of A375 cell line. The results of this study will help investigate the role of Nano microbubbles of Air, Oxygen and Ozone in the treatment of melanoma cancer.

Keywords


(1) de Waal, A., Cutaneous Melanoma. Population-based studies on epidemiological and clinical aspects. 2015, (Sl:
sn).
(2) Sheets, S.S.F., Melanoma of the Skin. National Cancer Insitute. Surveillance, Epidemiology and End Results
Program. Avalaible online :http://seer.cancer.gov/statfacts/html/melan.html, 2018.
(3) Goldstein, A.M. and M.A. Tucker, Dysplastic nevi and melanoma. 2013, AACR.
(4) Olsen, C.M., et al., Familial melanoma: a meta-analysis and estimates of attributable fraction. 2010. 19(1): p. 65-
73.
(5) Flaherty, K.T.J.A.r.o.m., Targeting metastatic melanoma. 2012. 63: p. 171-183.
(6) D'Orazio, J., et al., UV radiation and the skin. 2013. 14(6): p. 12222-12248.
(7) Guimarães, M.V., et al., Dry Extract of Matricaria recutita L.(Chamomile) Prevents Ligature‐Induced Alveolar
Bone Resorption in Rats via Inhibition of Tumor Necrosis Factor‐α and Interleukin‐1β. 2016. 87(6): p. 706-715.
(8) Temesgen, T., et al., Micro and nanobubble technologies as a new horizon for water-treatment techniques: A
review. 2017 :246 .p. 40-51.
(9) Wu, C., et al., Generation and characterization of submicron size bubbles. 2012. 179: p. 123-132.
(10) Agarwal, A., W.J. Ng, and Y.J.C. Liu, Principle and applications of microbubble and nanobubble technology for
water treatment. 2011. 8 :)9(4p. 1175-1180.
(11) Li, X., et al., Gas‐Liquid Mass Transfer Characteristics with Microbubble Aeration–I. Standard Stirred Tank.
2016. 39(5): p. 945-952.
(12) Xiong, Y., F.J.I.j.o.m.s. Peng, and technology, Optimization of cavitation venturi tube design for pico and nano
bubbles generation. 2015. 25(4): p. 523-529.
(13) Cho, K., et al., Therapeutic nanoparticles for drug delivery in cancer. 2008. 14(5): p. 1310-1316.
(14) Nikseresht, M., et al., The hydroalcoholic extract of Matricaria chamomilla suppresses migration and invasion of
human breast cancer MDA-MB-468 and MCF-7 cell lines. 2017. 9(1): p. 87.
(15) Naganuma, M., et al., Nanobubble technology to treat spinal cord ischemic injury. 2020. 3: p. 1-11.
(16) Ferrara, K.W., M.A. Borden, and H.J.A.o.c.r .Zhang, Lipid-shelled vehicles: engineering for ultrasound molecular
imaging and drug delivery. 2009. 42(7): p. 881-892.
(17) Zhang, Y., et al., Light-responsive CO 2 bubble-generating polymeric micelles for tumor cell ablation. 2017. 8(2):
p. 405-407.
(18) Shawli, H., et al., Nanobubble-enhanced antimicrobial agents: a promising approach for regenerative
endodontics. 2020. 46(9): p. 1248-1255.
(19) Seyedah Motahara Ghasemi, Ehsan Karimi and Hamidreza Rahimi, Investigation of the effects of air and oxygen
nano-microbubbles on cytotoxicity and induction of apoptosis in liver cancer cells (Huh7 grade), Journal of
Mashhad University of Medical Sciences Faculty of Medicine, 2018
(20) Mahjour, A., et al., Evaluation of antitumor effect of oxygen nanobubble water on breast cancer‐bearing BALB/c
mice. 2019. 120(9): p. 15546-1555.2