Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Effect of exercise activity with different intensity and volume on the expression of PDGF-B and FGF-2 genes in subcutaneous adipose tissue o f Wistar rats.

Document Type : Research Paper

Authors
Noshin Sarikhani 1
Majid Gholipour 2
Mohammad Malekipooya 3
1 Master of Sports Physiology, Karaj, Payame Noor University, Alborz, Iran.
2 2- Department of Physical Education, Sharif University of Technology, Tehran, Iran.
3 Department of Exercise Physiology, Professor Hesabi Branch, Islamic Azad University Professor Hesabi,Tafresh, Iran.(Corresponding Author)
10.22038/mjms.2022.63498.3722
Abstract
Background and Aim: Obesity is the cause of various diseases and in this regard, the growth of adipose tissue is associated with changes in angiogenesis. The effect of exercise on regulation of adipose tissue angiogenesis has been shown by some studies. The aim of the present study was to investigate the effect of exercise activity with different intensity and volume on the expression of PDGF-B and FGF-2 genes in subcutaneous adipose tissue of Wistar rats.
Materials and Methods: In this experimental study 24 rats (8 weeks old weighing 237±33 g) were kept under standard conditions and were randomly divided into 3 groups High Intensity Interval Training, Moderate Intensity Training and Control. HIIT (28 minutes, including 4 intervals of high intensity at 90 to 100% VO2max and low intensity at 50 to 60% VO2max) and MIT (37 minutes with an intensity of 65% VO2max) groups underwent 8 weeks exercise training. Tissue sampling was performed 24 hours after the last exercise training session and expression of PDGF-B and FGF-2 genes were measured by qRT-PCR. One-way ANOVA and Tukey post-hoc tests were used to analyze the data at the significance level of α <0.05.
Results: The results showed that HIIT and MIT significantly increased PDGF-B and FGF-2 gene expression in rat adipose tissue compared to the control group (both P=0.001).
Conclusion: It seems that according to the findings of the present study, both training protocols have the same effects on the process of angiogenesis of subcutaneous adipose tissue.
Keywords
High Intensity Interval Training
Moderate Intensity Training
Adipose Tissue
PDGF-B
FGF-2
  1. Scholtz S MJ. Obesity and psychiatry. Psychiatry. 2009;8(6):198-202.
    2. WHO. Global Recommendations on Physical Activity for Health Cataloguing-in-Publication Data2010. 2010:10-2 .
    3. Rojas- Rodriguez R GO, Kruse ME, Rosenthal B, Rao K, Min S, Bellve KD, Lif\shitz LM, Corvera S. Adipose tissue
    angiogenesis assay. . Method Enzymol. 2014;537:75-91.
    4. Hafidi ME, Buelna-Chontal, M., Sánchez-Muñoz, F., and Carbó, R. (). . Adipogenesis: a necessary but harmful
    strategy. Int J Mol Sci 2019:20:3657.
    5. Christiaens V LH. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol. 2010;318(1):2-9.
    6. Maleki Poya M. AB, Palizvan MR., et al. . The Effect of Eight Weeks of Incremental Endurance Training on The
    Levels of Matrix Metalloproteinase-1 (MMP1 )and Thrombosponidine-1 (TSP1) in the Rats, Induced by Myocardial
    Infarction by Isoproterenol. J Arak Uni Med Sci 2019;22(3):118-28.
    7. Tahergurabi Z KM. Obesity and Angiogenesis. . 2012;29(173):1-16.
    8. RJ. T. Response of the coronary vasculature to myocardial hypertrophy. Journal of the American College of
    Cardiology. 1990;15(3):528-33.
    9. Korivi M HC-W, Chen C-Y, Lee J-P, Kesireddy SR, Kuo C-H. . Angiogenesis: Role of exercise training and aging.
    Adapt Med. 2010;2:29-41.
    10.Lindberg N HE. PDGF in gliomas: more than just a growth factor? Ups J Med Sci 2012;117: 92-8.
    11.Cao R BE, Wahlestedt C, Thyberg J, Cao Y. . Leptin induces vascular permeability and synergistically stimulates
    angiogenesis.5–6390:)11(98 2001
    12.T. DBaY. Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats. . .
    Metabolism: clinical and experimental. 2014;63 (4): 452-5.
  2. 13.Czarkowska-Paczek B Z-PM, Bartlomiejczyk I, Przybylski J, Gorski J. The influence of physical exercise on the
    generation of TGF-β, PDGF-AA, and VEGF-A in adipose tissue. . Eur J Appl Physiol. 2011;5(111):875-81.
    14.Czarkowska PB BI, Przybylski J. . The serum levels of growth factors: PDGF, TGF-beta and VEGF are increased
    after strenuous physical exercise. J Physiol Pharmacol 2006;57:189-97.
    15.Barari A BJ, Sarabandi M. . The effect of circuit resistance training combined with ginseng supplementation level of
    VEGF and PDGF in inactive females. Med J Tabriz Univ Med Sci Health Serv. 2015;37:6-13.
    16.Li D-J FH, Zhao T, Ni M, Shen F-M. . . Exercise-stimulated FGF23 promotes exercise performance via controlling
    the excess reactive oxygen species production and enhancing mitochondrial function in skeletal muscle. Metabolism:
    clinical and experimental 2016;65(5):747-5.6
    17.Cheragh Birjandi S SM, Hedayati M. Effect of high intensity interval training and L-Arginine supplementation on
    serum levels of fibroblast growth factor 21 and atrial natriuretic peptide in overweight and obese young men. . Journal
    of Birjand University of Medical Sciences ; (): [Persian]. 2016;23(3):211-21.
    18.Wisløff U HJ, Kemi OJ, Ellingsen Ø. Intensity-controlled treadmill running in rats: Vo2max and cardiac hypertrophy.
    . Am J Physiol Heart Circ Physiol ;. 2001; 280(3):H1301–10.
    19.Rognmo Ø ,Hetland, E., Helgerud, J., Hoff, J. and Slørdahl, S.A., . High intensity aerobic interval exercise is superior
    to moderate intensity exercise for increasing aerobic capacity in patients with coronary artery disease. European
    Journal of Cardiovascular Prevention & Rehabilitation. 2004;11(3):.216-22.
    20.Kregel KC, Allen, D.L., Booth, F.W., Fleshner, M.R., Henrikson, E.J., Musch, T.I., O’Leary, D.S., Parks, C.M., Poole,
    D.C., Ra’anan, A.W. and Sheriff, D.D., . . Resource book for the design of animal exercise protocols. American
    Physiological Society, . Bethesda, MD, USA. 2006.
    21.Trayhurn P WI. Adipokines: inflammation and the pleiotropic role of white adipose tissue. Br J Nutr. 2004;92:347–55.
    22.Molgat AS GA, Sorisky A. . Preadipocyte apoptosis is prevented by macrophage-conditioned medium in a
    PDGFdependent manner. Am J Physiol Cell Physiol 2009;296:C757–C65.
    23.Pallua N PA, Suschek C, Wolter TP. . Content of the growth factors bFGF, IGF-1, VEGF, and PDGF-BB in freshly
    harvested lipoaspirate after centrifugation and incubation:Plast Reconstr Surg 2009;123:826–33.
    24.Rahimi M GS, Asad MR, Abbasi A. The effect of exercise with different intensity on platelet- derived growth factor
    gene expression in visceral and subcutaneous adipose tissue of rats . .Rep Health Care. 2017; 3 (3): 37- 45.
    25.Mirzaei Amirabad G AMR, Rahimi M. . The Effect of Exercise with Different Intensity and Volume on fibroblast
    growth factor (FGF-2) Gene Expression in Subcutaneous and Visceral Adipose tissue in Male Rats. J Of Sport and
    Exercise Physiology. 2018;11(2):103-16.
    26.Trenerry MK DGP, Larsen AE, Garnham AP, Cameron‐ Smith D. . Impact of resistance exercise training on
    interleukin‐ 6 and JAK/STAT in young men. Muscle Nerve. 2011;43:385-92.
    27.Czarkowska-Paczek B Z-PM, Bartlomiejczyk I, Przybylski J, Gorski J. . Skeletal and heart muscle expression of
    PDGF-AA and VEGF-A after an acute bout of exercise and endurance training in rats. . Med Sci Monit.
    2010;16:BR147-53.
    28.Li X EU. Novel VEGF family members: VEGF-B ,VEGF-C and VEGF-D. . Int J Biochem Cell Boil 2001;3:421-6.
    29.Nameni F NMMS. The effect of endurance resistance exercise combined with Lcitrulline malate supplement on FGF,
    VEGF, and fatigue among young bodybuilders. . Ebnesina J. 2020;21(4.)
    30.Ramos JS DL, Tjonna AE, Beetham KS, Coombes JS. . The impact of high-intensity interval training versus moderateintensity continuous training on vascular function: a systematic review and meta-analysis. Sports Med.
    2015;45(5):679-92.
    31.Ye J GZ, Yin J, He Q. Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose
    tissue of ob/ob and dietary obese mice. Am J Physiol-Endoc M 2007;293 (4):E1118-E28. .
    32.Shweiki D IA, Soffer D, Keshet E. . Vascular endothelial growth factor induced by hypoxia may mediate hypoxiainitiated angiogenesis. . Nature. 1992;359(6398):843-5.
    33.Lundby C GM, Pilegaarg H. . Regular endurance training reduces the exercise induced HIF-1alpha and HIF- 2alpha
    mRNA expression in human skeletal muscle in normoxic conditions. Eur J Appl Physiol. 2006;96:363–9.
    34.Olfert IM HR, Wagner PD, Breen EC. . Myocyte vascular endothelial growth factor is required for exercise-induced
    skeletal muscle angiogenesis. American journal of physiology.
    35. .Regulatory, integrative and comparative physiology ;. 2010;299(4):R1059-R67.
    36.Loustau T CE, Karkeni E, Jover B, Laurant P, Landrier J-F, Riva C. Physical exercise regulates angiogenesis in
    adipose tissue of dietinduced obese mice thru ECSCR/Akt pathways. . Arch Cardiovasc Dis. 2016;8(3):219-20.
    37.Coker RH WR, Kortebein PM, Sullivan DH, Evans WJ. . Influence of exercise intensity on abdominal fat and
    adiponectin in elderly adults. . Metab Syndr Relat Disord. 2009; 7(4):363-8.
    38.Ohkawara K TS, Miyachi M, Ishikawa-Takata K, Tabata I. A dose–response relation between aerobic exercise and
    visceral fat reduction: systematic review of clinical trials. . Int J Obesity ; 31(12): . 2007;31:1786-97.
Medical Journal of Mashhad university of Medical Sciences
Volume 65, Issue 3
May and June 2022
Pages 1259-1267