The Effect of High Intensity Interval Resistance Training and Vitamin D Supplementation on Sirtuin1 and Interleukin-10 levels in Overweight Women with Low Vitamin D Status

Document Type : Research Paper

Authors

1 PhD Student in Sports Physiolog, Dept. of Physical Education and Sports Sciences, Sari Branch, Islamic Azad University, Sari, Iran

2 Assistant Prof., Dept. of Physical Education and Sports Sciences, Sari Branch, Islamic Azad University, Sari, Iran

3 Associate Prof., Dept. of Physical Education and Sports Sciences, Qaemshahar Branch, Islamic Azad University, Qaemshahar, Iran

Abstract

Introduction: Overweight and obesity are associated with low-grade chronic inflammation, and the study of interventions affecting inflammation-reducing signaling pathways is a potential therapeutic goal in vitamin D (VD) deficiency-induced diseases. The aim of this study was to evaluate the effect of high intensity interval resistance training (HIIRT) and VD supplementation on Sirtuin 1(SIRT1) and Interleukin-10 (IL10) levels in overweight women.
Materials and Methods: This semi-experimental study was performed on 52 overweight women with VD deficiency (26.04±1.95 years and BMI=27.42±1.10 kg/m2). Subjects were purposefully selected and then randomly divided into exercise, VD, exercise +VD (combined) and control groups. HIIRT consisted of three sets with an intensity of 80% of one maximum repetition, and 150 seconds of rest between sets for 8 weeks. VD was taken weekly at a dose of 50,000 units. Data were analyzed using paired t-test, one-way ANOVA and Kruskal-Wallis at P<0.05.
Results: After 8 weeks of VD supplementation, HIIRT and combined intervention, SIRT1 and IL10 levels increased significantly (P<0.001). In addition, there was a significant difference between the rate of changes in IL10 and SIRT1 levels in the combined group with the exercise (P=0.004 and P<0.001, respectively) and VD (P<0.001) groups and also in the exercise group with the VD supplement group (P=0.004 and P=0.008, respectively).
Conclusion: It seems that HIIRT is more effective in increasing SIRT1 and IL10 and thus reducing inflammation and its complications in overweight conditions with low VD status, so that concurrent exercise interventions and VD consumption is associated with reinforcement these effects.

Keywords

References

  1. Pirola L, Ferraz JC. Role of pro- and anti-inflammatory phenomena in the physiopathology of type 2 diabetes and obesity. World journal of biological chemistry 2017;8(2):120-128. doi: 10.4331/wjbc.v8.i2.120
  2. El Ouaamari A, Minehira K. Nonalcoholic Fatty liver disease: its mechanisms and complications. International journal of endocrinology 2013;2013:969748. doi: 10.1155/2013/969748
  3. Yilma AN, Singh SR, Fairley SJ, Taha MA, Dennis VA. The anti-inflammatory cytokine, interleukin-10, inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV-inactivated Chlamydia trachomatis. Mediators of inflammation 2012;2012:520174. doi: 10.1155/2012/520174
  4. Abdelhamid ER, Kamhawy AH, Ahmed HH, Abu Shady  MM, Eladawy R, Megawer  AS, et al. Role of Inflammatory Cytokines in Obese and Nonobese Diabetic Children. Open Access Macedonian Journal of Medical Sciences 2020; 8(B):858-865. doi:10.3889/oamjms.2020.4982.
  5. Lauridsen JK, Olesen RH, Vendelbo J, Hyde TM, Kleinman JE, Bibby BM, et al. High BMI levels associate with reduced mRNA expression of IL10 and increased mRNA expression of iNOS (NOS2) in human frontal cortex. Translational psychiatry 2017;7(2):e1044. doi: 10.1038/tp.2016.259
  6. Fjeldborg K, Pedersen SB, Moller HJ, Christiansen T, Bennetzen M, Richelsen B. Human adipose tissue macrophages are enhanced but changed to an anti-inflammatory profile in obesity. Journal of immunology research 2014; 2014: 309548. doi: 10.1155/2014/309548
  7. Dorneles GP, Haddad DO, Fagundes VO, Vargas BK, Kloecker A, Romao PR, et al. High intensity interval exercise decreases IL-8 and enhances the immunomodulatory cytokine interleukin-10 in lean and overweight-obese individuals. Cytokine 2016; 77: 1–9.. doi: 10.1016/j.cyto.2015.10.003
  8. Chang JS, Chang CC, Chien E, Lin SS, Cheng-Shiuan T, Bai CH, Chao KC. Association between interleukin 1β and interleukin 10 concentrations: a cross-sectional study in young adolescents in Taiwan. Pediatrics 2013, 13:123. doi: 10.1186/1471-2431-13-123.
  9. Sabir MS, Khan Z, Hu C, Galligan MA, Dussik CM, Mallick S, et al. SIRT1 enzymatically potentiates 1,25-dihydroxyvitamin D3 signaling via vitamin D receptor deacetylation. Journal of Steroid Biochemistry and Molecular Biology 2017;172:117-129. doi: 10.1016/j.jsbmb.2017.06.010
  10. Song YS, Lee SK, Jang YJ, Park HS, Kim JH, Lee YJ, et al. Association between low SIRT1 expression in visceral and subcutaneous adipose tissues and metabolic abnormalities in women with obesity and type 2 diabetes. Diabetes Research and Clinical Practice 2013;101(3):341-8. doi: 10.1016/j.diabres.2013.07.002
  11. Arab Sadeghabadi Z, Nourbakhsh M, Pasalar P, Emamgholipour S, Golestani A, Larijani B, et al. Reduced gene expression of sirtuins and active AMPK levels in children and adolescents with obesity and insulin resistance. Obesity Research and Clinical Practice 2018 (2):167-173. doi: 10.1016/j.orcp.2017.10.004.
  12. Ye X, Li M, Hou T, Gao T, Zhu WG, Yang Y. Sirtuins in glucose and lipid metabolism. Oncotarget 2017;8(1):1845-59. doi: 10.18632/oncotarget.12157
  13. Abiri B, Vafa M. Effects of vitamin D and/or magnesium supplementation on mood, serum levels of BDNF, inflammatory biomarkers, and SIRT1 in obese women: a study protocol for a double-blind, randomized, placebo-controlled trial. Trials 2020;21(1):1-7. doi: 10.1186/s13063-020-4122-9
  14. Yin K, Agrawal DK. Vitamin D and inflammatory diseases. Journal of inflammation research 2014;7:69-87. doi: 10.2147/JIR.S63898
  15. Vranić L, Mikolašević I, Milić S. Vitamin D Deficiency: Consequence or Cause of Obesity? Medicina (Kaunas, Lithuania) 2019;55(9):541. doi: 10.3390/medicina55090541
  16. Blum M, Dolnikowski G, Seyoum E, Harris SS, Booth SL, Peterson J, et al. Vitamin D (3) in fat tissue. Endocrine 2008;33(1):90-94. doi: 10.1007/s12020-008-9051-4
  17. Kim HA, Perrelli A, Ragni A, Retta F, De Silva TM, Sobey CG, et al. Vitamin D Deficiency and the Risk of Cerebrovascular Disease. Antioxidants 2020;9(4):327. doi: 10.3390/antiox9040327
  18. Chang E, Kim Y. Vitamin D insufficiency exacerbates adipose tissue macrophage infiltration and decreases AMPK/SIRT1 activity in obese rats. Nutrients 2017;9(4):338. doi: 10.3390/nu9040338
  19. Aliashrafi S, Arefhosseini SR, Lotfi-Dizaji L, Ebrahimi-Mameghani M. Effect of vitamin D supplementation in combination with weight loss diet on lipid profile and sirtuin 1 in obese subjects with vitamin D deficiency: a double blind randomized clinical trial. Health promotion perspectives 2019;9(4):263-69. doi: 10.15171/hpp.2019.36. eCollection 2019
  20. Galan-Lopez P, Sanchez-Oliver AJ, Pihu M, Gísladóttír T, Domínguez R, Ries F. Association between Adherence to the Mediterranean Diet and Physical Fitness with Body Composition Parameters in 1717 European Adolescents: The AdolesHealth Study. Nutrients 2019;12(1):77. doi: 10.3390/nu12010077
  21. Gonzalez-Molero I, Rojo-Martinez G, Morcillo S, Gutierrez C, Rubio E, Perez-Valero V, et al. Hypovitaminosis D and incidence of obesity: a prospective study. European journal of clinical nutrition 2013; 67(6):680–2. doi: 10.1038/ejcn.2013.48
  22. Beavers KM, Brinkley TE, Nicklas BJ. Effect of exercise training on chronic inflammation. Clinica Chimica Acta 2010;411(11-12):785-93. doi: 10.1016/j.cca.2010.02.069.
  23. Kalhor F, Arshadi S, Zfari A,  Banaeifar A. The effect of resistance training on Atrogin, eotaxin and IL-10 indices in obese wome. Razi Journal of Medical Sciences 2020;27(3):130-137.[ In Persian]
  24. Khodadoust M, Habibian M. [Investigating the Changes of Tumor Necrosis Factor-α and Interleukin-10 After 8 Weeks of Regular Pilates Exercise and Vitamin D Intake in Overweight Men: A Randomized Clinical Trial]. Journal of Arak University Medical Sciences 2020; 23 (6) :888-901.doi:32598/jams.23.6.3537.5 [ In Persian]
  25. Mombini H, Eslami Farsani M, Ab Abzadeh Sh, Barzegar H, Vahdat H. [Effect of High-intensity Interval Training (HIIT) on the levels of irisin and Interleukin-10 in overweight men]. Qom University of Medical Sciences journal 2018;12(2):35-44.[ In Persian]
  26. Ghasemi E, Afzalpou. ME, Zarban A. [The Effects of 10 Weeks of High Intensity Interval Training and Green Tea Supplementation on Serum Levels of Sirtuin 1 and Catalase in Overweight Women]. Sport Physiolog 2017; 8 (32): 169-84.[ In Persian]
  27. Zakavi I, Nayebifar S, Ghasemi E, Valipour A. Therapeutic Efficacy of Aerobic Exercise Training along with Oak Husk Hydroalcoholic Extract for Amelioration of Inflammation in Obese Elderly Male Mice. BioMed research international 2021;2021:5585077. doi: 10.1155/2021/5585077.
  28. Moro T, Marcolin G, Bianco A, Bolzetta F, Berton L, Sergi G, et al. Effects of 6 Weeks of Traditional Resistance Training or High Intensity Interval Resistance Training on Body Composition, Aerobic Power and Strength in Healthy Young Subjects: A Randomized Parallel Trial. International Journal of Environmental Research and Public Health 2020;17(11):4093. doi: 10.3390/ijerph17114093.
  29. Rahimian Mashhad Z, Attarzadeh Hosseini S, Rashid Lamirm A, Sardar M, Nekooei S, Giti R. Effect of aerobic and resistance exercise programs on arterial stiffness, serum IL6 and IL10 in obese women. The Iranian Journal of Obstetrics, Gynecology and Infertility 2020; 23(2):20-29. [In Persian]
  30. Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. Journal of applied physiology 2005;98(4):1154-62.
  31. Ma JK, Scribbans TD, Edgett BA, Boyd JC, Simpson CA, Little JP, et al. Extremely low-volume, high-intensity interval training improves exercise capacity and increases mitochondrial protein content in human skeletal muscle. Open Journal of Molecular and Integrative Physiology 2013; 3(4): 202-10. doi: 10.4236/ojmip.2013.34027
  32. Akbari M, Ostadmohammadi V, Lankarani KB, Tabrizi R, Kolahdooz F, Heydari ST, et al. The Effects of Vitamin D Supplementation on Biomarkers of Inflammation and Oxidative Stress Among Women with Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Hormone and Metabolic Research 2018;50(4):271-79. doi: 10.1055/s-0044-101355
  33. Torma F, Gombos Z, Jokai M, Takeda M, Mimura T, Radak Z. High intensity interval training and molecular adaptive response of skeletal muscle. Sports Medicine and Health Science 2019; 1(1):24-32. doi:10.1016/j.smhs.2019.08.003
  34. Vargas-Ortiz K, Pérez-Vázquez V, Macías-Cervantes MH. Exercise and Sirtuins: A Way to Mitochondrial Health in Skeletal Muscle. International journal of molecular sciences 2019;20(11):2717. doi: 10.3390/ijms20112717
  35. Ruiz-Ojeda FJ, Anguita-Ruiz A, Leis R, Aguilera CM. Genetic factors and molecular mechanisms of vitamin D and obesity relationship. Annals of Nutrition and Metabolism 2018;73(2):89-99. doi: 10.1159/000490669
  36. Chen Y, Zhang J, Ge X, Du J, Deb DK, Li YC. Vitamin D Receptor Inhibits Nuclear Factor κB Activation by Interacting with IκB Kinase β Protein. International journal of biological chemistry 2013; 288(27): 19450-8. doi: 10.1074/jbc.M113.467670
  37. Zhang X, Zhou M, Guo Y, Song Z, Liu B. 1,25-Dihydroxyvitamin D₃ Promotes High Glucose-Induced M1 Macrophage Switching to M2 via the VDR-PPARγ Signaling Pathway. BioMed research international 2015;2015:157834. doi: 10.1155/2015/157834
  38. Ren Z, He H, Zuo Z, Xu Z, Wei Z, Deng J. The role of different SIRT1-mediated signaling pathways in toxic injury. Cellular and Molecular Biology Letters 2019 30;24:36. doi: 10.1186/s11658-019-0158-9
  39. Zhang N, Li P, Lin H, Shuo T, Ping F, Su L, et al. IL-10 ameliorates PM2.5-induced lung injury by activating the AMPK/SIRT1/PGC-1α pathway. Environmental Toxicology and Pharmacology 2021;86:103659. doi: 10.1016/j.etap.2021.103659
Medical Journal of Mashhad university of Medical Sciences
Volume 66, Issue 2
July and August 2023
Pages 178-188

Files

Share

How to cite

Statistics