تغییرات سطح کمرین و مقاومت به انسولین موش های صحرایی مبتلا به کبد چرب غیر الکلی: اثر تمرینات تناوبی شدید و مکمل گیری عصاره خرفه

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران.

چکیده

هدف از این تحقیق بررسی تاثیر تمرینات تناوبی شدید (HIIT) و مصرف عصاره خرفه بر سطح کمرین کبدی و مقاومت به انسولین موش های صحرایی مبتلا به NAFLD بود. تعداد 25 موش نر بالغ نژاد ویستار با سن شش هفته با وزن 160 تا 185 گرم در 5 گروه کنترل سالم، کنترل کبدچرب، تمرین، عصاره و تمرین+عصاره بطور تصادفی قرار داده شدند. با توجه به وزن موش ها مکمل خرفه با دوز mg/kg 400 به دو گروه مربوطه خورانده شد. تمرین‌های تناوبی شدید (نیز بمدت 8 هفته ، 5 جلسه در هفته و هر جلسه 7 تکرار یک دقیقه ای باشدت ۹۰ درصد سرعت بیشینه انجام شد که تناوب های استراحت فعال نیز شامل 2 دقیقه دویدن با شدت ۲۰ درصد بیشینه بود. سطح کمرین کبدی به روش الایزا و مقاومت به انسولین سرمی مورد ارزیابی قرار گرفت. مقاومت به انسولین در گروه کنترل کبد چرب غیرالکلی به طور معنی داری نسبت به گروه سالم کنترل بالاتر بود (002/0=p). سطح این شاخص در گروه تمرین (01/0=p)، عصاره خرفه (037/0=p) و تمرین+عصاره (012/0=p) به طور معنی داری پایین‌تر از گروه کنترل کبد چرب بود. سطح کمرین کبدی در گروه تمرین تمرین (010/0=p)، مصرف عصاره خرفه (017/0=p) و تمرین+عصاره (001/0=p) به طور معنی داری پایین تر از گروه کنترل کبد چرب بود. احتمالاً 8 هفته تمرین ورزشی HIIT و مصرف عصاره خرفه از طریق کاهش سطح کمرین و مقاومت به انسولین در بیماران مبتلا به NAFLD می تواند نقش مهمی را در کنترل پیشرفت این بیماری بر عهده داشته باشند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Changes in Chemerin Level and Insulin Resistance in Rats with Non-Alcoholic Fatty Liver Disease: Effect of High Intensity Interval Training and Portulaca Oleracea extract

نویسندگان [English]

  • mohammad Ranaei
  • Ali Yaghoubi
  • Sadegh cheragh-birjandi
Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.
چکیده [English]

the aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and Portulaca Oleracea extract on chemerin level and insulin resistance in rats with NAFLD. Materials and methods: Twenty five male Wistar rats were randomly divided into five groups: healthy control, fatty liver control, HIIT, Portulaca Oleracea extract, and HIIT+Portulaca Oleracea extract. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. Portulaca Oleracea supplement at 400 mg/kg was given to the experimental groups. HIIT was performed for 8 weeks, 5 sessions per week with 7 repetitions of 1 minute at 90% maximum speed accompanied by active rest intervals including 2 minutes of running at 20% maximum speed. Hepatic chemerin level and insulin resistance were measured after 8 weeks of HIIT and consumption of purslane extract. Results: Insulin resistance was significantly higher in fatty liver control group than healthy controls (P=0.002). But it was significantly lower in HIIT group (P=0.01), Portulaca Oleracea extract (P=0.037), and HIIT+Portulaca Oleracea extract group (P=0.012) than that in fatty liver control group. Chemerin levels in the HIIT group (p=0.01), Portulaca Oleracea extract (p=0.017) and HIIT+ Portulaca Oleracea extract (p=0.001) was significantly lower than the fatty liver control group. Conclusion: It seems that HIIT and Portulaca Oleracea extract can play an important role in controlling the progression of this disease by reducing the level of chemerin and insulin resistance in patients with NAFLD.

کلیدواژه‌ها [English]

  • High intensity interval training
  • Portulaca Oleracea extract
  • chemerin
  • insulin resistance
  • non-alcoholic fatty liver disease

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 65، شماره 6
بهمن و اسفند 1401
صفحه 2273-2284

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