Changes in Chemerin Level and Insulin Resistance in Rats with Non-Alcoholic Fatty Liver Disease: Effect of High Intensity Interval Training and Portulaca Oleracea extract

Document Type : Research Paper

Authors

1 Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.

2 Department of Sport Sciences, Islamic Azad University, Bojnurd branch, Bojnurd, Iran

Abstract

the aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and Portulaca Oleracea extract on chemerin level and insulin resistance in rats with NAFLD. Materials and methods: Twenty five male Wistar rats were randomly divided into five groups: healthy control, fatty liver control, HIIT, Portulaca Oleracea extract, and HIIT+Portulaca Oleracea extract. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. Portulaca Oleracea supplement at 400 mg/kg was given to the experimental groups. HIIT was performed for 8 weeks, 5 sessions per week with 7 repetitions of 1 minute at 90% maximum speed accompanied by active rest intervals including 2 minutes of running at 20% maximum speed. Hepatic chemerin level and insulin resistance were measured after 8 weeks of HIIT and consumption of purslane extract. Results: Insulin resistance was significantly higher in fatty liver control group than healthy controls (P=0.002). But it was significantly lower in HIIT group (P=0.01), Portulaca Oleracea extract (P=0.037), and HIIT+Portulaca Oleracea extract group (P=0.012) than that in fatty liver control group. Chemerin levels in the HIIT group (p=0.01), Portulaca Oleracea extract (p=0.017) and HIIT+ Portulaca Oleracea extract (p=0.001) was significantly lower than the fatty liver control group. Conclusion: It seems that HIIT and Portulaca Oleracea extract can play an important role in controlling the progression of this disease by reducing the level of chemerin and insulin resistance in patients with NAFLD.

Keywords

Main Subjects

References

  1. Chalasani N, Younossi Z, Lavine JE, Diehl AM, Brunt EM, Cusi K, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the American Gastroenterological Association, American Association for the Study of Liver Diseases, and American College of Gastroenterology. Gastroenterology. 2012;142(7):1592-609.
  2. Loomba R, Sanyal AJ. The global NAFLD epidemic. Nature reviews Gastroenterology & hepatology. 2013;10(11):686-90.
  3. Watt MJ, Miotto PM, De Nardo W, Montgomery MK. The liver as an endocrine organ—linking NAFLD and insulin resistance. Endocrine reviews. 2019;40(5):1367-93.
  4. Portillo-Sanchez P, Bril F, Maximos M, Lomonaco R, Biernacki D, Orsak B, et al. High prevalence of nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus and normal plasma aminotransferase levels. The journal of clinical endocrinology & metabolism. 2015;100(6):2231-8.
  5. Masarone M, Rosato V, Aglitti A, Bucci T, Caruso R, Salvatore T, et al. Liver biopsy in type 2 diabetes mellitus: Steatohepatitis represents the sole feature of liver damage. PLoS One. 2017;12(6):e0178473.
  6. Bugianesi E, Gastaldelli A, Vanni E, Gambino R, Cassader M, Baldi S, et al. Insulin resistance in non-diabetic patients with non-alcoholic fatty liver disease: sites and mechanisms. Diabetologia. 2005;48(4):634-42.
  7. Korenblat KM, Fabbrini E, Mohammed BS, Klein S. Liver, muscle, and adipose tissue insulin action is directly related to intrahepatic triglyceride content in obese subjects. Gastroenterology. 2008;134(5):1369-75.
  8. Peng L, Yu Y, Liu J, Li S, He H, Cheng N, et al. The chemerin receptor CMKLR1 is a functional receptor for amyloid-β peptide. Journal of Alzheimer's Disease. 2015;43(1):227-42.
  9. Ernst MC, Sinal CJ. Chemerin: at the crossroads of inflammation and obesity. Trends in Endocrinology & Metabolism. 2010;21(11):660-7.
  10. Rourke J, Dranse H, Sinal C. Towards an integrative approach to understanding the role of chemerin in human health and disease. Obesity Reviews. 2013;14(3):245-62.
  11. Buechler C, Feder S, Haberl EM, Aslanidis C. Chemerin isoforms and activity in obesity. International journal of molecular sciences. 2019;20(5):1128.
  12. Sell H, Laurencikiene J, Taube A, Eckardt K, Cramer A, Horrighs A, et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes. 2009;58(12):2731-40.
  13. Bozaoglu K, Bolton K, McMillan J, Zimmet P, Jowett J, Collier G, et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology. 2007;148(10):4687-94.
  14. Kukla M, Zwirska-Korczala K, Hartleb M, Waluga M, Chwist A, Kajor M, et al. Serum chemerin and vaspin in non-alcoholic fatty liver disease. Scandinavian journal of gastroenterology. 2010;45(2):235-42.
  15. Yilmaz Y, Yonal O, Kurt R, Alahdab YO, Eren F, Ozdogan O, et al. Serum levels of omentin, chemerin and adipsin in patients with biopsy-proven nonalcoholic fatty liver disease. Scandinavian journal of gastroenterology. 2011;46(1):91-7.
  16. Marques C, Motta V, Torres T, Aguila M, Mandarim-de-Lacerda C. Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice. Brazilian journal of medical and biological research. 2010;10:467-75.
  17. Hottenrott K, Ludyga S, Schulze S. Effects of high intensity training and continuous endurance training on aerobic capacity and body composition in recreationally active runners. Journal of sports science & medicine. 2012;11(3):483.
  18. Nalcakan GR. The effects of sprint interval vs. continuous endurance training on physiological and metabolic adaptations in young healthy adults. Journal of human kinetics. 2014;44:97.
  19. Gibala MJ, Little JP, MacDonald MJ, Hawley JA. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of physiology. 2012;590(5):1077-84.
  20. Francois ME, Little JP. Effectiveness and safety of high-intensity interval training in patients with type 2 diabetes. Diabetes Spectrum. 2015;28(1):39-44.
  21. Kalaki-Jouybari F, Shanaki M, Delfan M, Gorgani-Firouzjae S, Khakdan S. High-intensity interval training (HIIT) alleviated NAFLD feature via miR-122 induction in liver of high-fat high-fructose diet induced diabetic rats. Archives of physiology and biochemistry. 2020;126(3):242-9.
  22. Zhou Y-X, Xin H-L, Rahman K, Wang S-J, Peng C, Zhang H. Portulaca oleracea L.: a review of phytochemistry and pharmacological effects. BioMed research international. 2015;2015.
  23. Chan K, Islam M, Kamil Ma, Radhakrishnan R, Zakaria M, Habibullah M, et al. The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. sativa (Haw.) Celak. Journal of ethnopharmacology. 2000;73(3):445-51.
  24. Malek F, Boskabady M, Borushaki MT, Tohidi M. Bronchodilatory effect of Portulaca oleracea in airways of asthmatic patients. Journal of ethnopharmacology. 2004;93(1):57-62.
  25. Gheflati A, Adelnia E, Nadjarzadeh A. The clinical effects of purslane (Portulaca oleracea) seeds on metabolic profiles in patients with nonalcoholic fatty liver disease: A randomized controlled clinical trial. Phytotherapy Research. 2019;33(5):1501-9.
  26. Zarei A, Changizi Ashtiyani S, Taheri S. The effects of hydroalcoholic extract of Portulaca Oleracea on the serum concentreation of Hepatic enzymes in Rats. ISMJ. 2014;17(5):889-99.
  27. Lan S, Fu-er L. Effects of Portulaca oleracea on insulin resistance in rats with type 2 diabetes mellitus. Chinese Journal of Integrative Medicine. 2003;9(4):289-92.
  28. Dehbashi M, Fathie M, Attarzadeh HSR, Mosaferi ZM. The Effect Of Eight Weeks Of Endurance Training And Injection Of Growth Hormone Lipolytic Fragment (Aod9604) On Ck18 And Liver Enzymes Of Nafld-Induced Mice Induced By High-Fat Diet. 2021.
  29. Barjasteyazdy A, Zarei M. The effect of high intensity interval training (HIIT) with portulaca Oleracea supplementation on serum levels of liver enzyme in rats with non-alcoholic fatty live disease. Journal of Sport and Biomotor Sciences. 2021;26(26):56-65.
  30. Hafstad AD, Lund J, Hadler-Olsen E, Höper AC, Larsen TS, Aasum E. High-and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes. 2013;62(7):2287-94.
  31. Hafstad AD, Boardman NT, Lund J, Hagve M, Khalid AM, Wisløff U, et al. High intensity interval training alters substrate utilization and reduces oxygen consumption in the heart. Journal of Applied Physiology. 2011;111(5):1235-41.
  32. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 2007;14(6):753-60.
  33. Darvish Damavandi R, Shidfar F, Najafi M, Janani L, Masoodi M, Akbari‐Fakhrabadi M, et al. Effect of Portulaca Oleracea (purslane) extract on liver enzymes, lipid profile, and glycemic status in nonalcoholic fatty liver disease: A randomized, double‐blind clinical trial. Phytotherapy Research. 2021.
  34. Do GM, Oh HY, Kwon EY, Cho Yy, Shin Sk, Park HJ, et al. Long‐term adaptation of global transcription and metabolism in the liver of high‐fat diet‐fed C57BL/6J mice. Molecular nutrition & food research. 2011;55(S2):S173-S85.
  35. Brown MS, Goldstein JL. Selective versus total insulin resistance: a pathogenic paradox. Cell metabolism. 2008;7(2):95-6.
  36. Chen G, Liang G, Ou J, Goldstein JL, Brown MS. Central role for liver X receptor in insulin-mediated activation of Srebp-1c transcription and stimulation of fatty acid synthesis in liver. Proceedings of the National Academy of Sciences. 2004;101(31):11245-50.
  37. Macavei B, Baban A, Dumitrascu D. Psychological factors associated with NAFLD/NASH: a systematic review. Eur Rev Med Pharmacol Sci. 2016;20(24):5081-97.
  38. Cho J, Lee I, Kim D, Koh Y, Kong J, Lee S, et al. Effect of aerobic exercise training on non-alcoholic fatty liver disease induced by a high fat diet in C57BL/6 mice. Journal of exercise nutrition & biochemistry. 2014;18(4):339.
  39. Lehrke M, Becker A, Greif M, Stark R, Laubender RP, von Ziegler F, et al. Chemerin is associated with markers of inflammation and components of the metabolic syndrome but does not predict coronary atherosclerosis. European journal of endocrinology. 2009;161(2):339.
  40. Zhuang X, Sun F, Li L, Jiang D, Li X, Sun A, et al. Therapeutic Effect of Metformin on Chemerin in Non-Obese Patients with Non-Alcoholic Fatty Liver Disease (NAFLD). Clinical laboratory. 2015;61(10):1409-14.
  41. Docke S, Lock J, Birkenfeld A, Hoppe S, Lieske S, Rieger A, et al. Elevated hepatic chemerin mRNA expression in human non-alcoholic fatty liver disease. Eur J Endocrinol. 2013;169(5):547-57.
  42. Zahraei H, Mogharnasi M, Afzalpour ME, Fanaei H. The effect of 8 weeks of continuous and high intensity interval swimming on chemerin levels in liver and visceral fat tissues and insulin resistance in male rats with metabolic syndrome. Journal of Sport and Exercise Physiology. 2022;15(1):33-44.
  43. Hosseini M, Eftekhar B, Riyahi Malayeri S. Effect of interval training with curcumin consumption on some adipokines in menopausal obese rats. Journal of Rafsanjan University of Medical Sciences. 2017;16(6):505-16.
  44. Mahmoudi A, Siauhkouhian M, Iranparvar M, Anari H, Seifi F. Plasma changes of chemerin and pentraxin-3 following eight weeks of endurance exercise in men with non-alcoholic fatty liver disease. Journal of Ardabil university of medical sciences. 2018;17(4):476-86.
  45. Asad MR, Kheradmand S, Kheradmand N. Comparing the Effect of Endurance Exercise and High-Intensity Interval Exercise on Plasma Levels of Chemerin and Insulin Resistance in Obese Male Rats. Journal of Arak University of Medical Sciences. 2019;22(5):112-23.
  46. Fathi R, Mosayebi Z, Nazarali P, Aslani S. Effect of resistance training on plasma levels of chemerin and Insulin in two groups of healthy and insulin resistance male rats. Research in Medicine. 2015;38(4):207-13.
  47. Parastesh M, Nadi Z. The Effects of Regular Resistance Training on the Liver’s Inflammatory Indexes, Chemerin, Resistin, and Insulin Resistance Index in Healthy and Type 2 Diabetic Male Rats. Journal of Arak University of Medical Sciences. 2020;23(1):48-59.
  48. Hayoz D, Ziegler T, Brunner HR, Ruiz J. Diabetes mellitus and vascular lesions. Metabolism. 1998;47:16-9.
  49. Zhu H, Wang Y, Liu Y, Xia Y, Tang T. Analysis of flavonoids in Portulaca oleracea L. by UV–vis spectrophotometry with comparative study on different extraction technologies. Food Analytical Methods. 2010;3(2):90-7.
  50. Chen B, Zhou H, Zhao W, Zhou W, Yuan Q, Yang G. Effects of aqueous extract of Portulaca oleracea L. on oxidative stress and liver, spleen leptin, PARα and FAS mRNA expression in high-fat diet induced mice. Molecular biology reports. 2012;39(8):7981-8.
  51. Karimi G, Aghasizadeh M, Razavi M, Taghiabadi E. Protective effects of aqueous and ethanolic extracts of Nigella sativa L. and Portulaca oleracea L. on free radical induced hemolysis of RBCs. Daru: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences. 2011;19(4):295.
  52. Alam M, Juraimi AS, Rafii M, Abdul Hamid A, Aslani F, Hasan M, et al. Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected purslane (Portulaca oleracea L.) accessions. BioMed research international. 2014;2014.
  53. Uddin M, Juraimi AS, Hossain MS, Nahar M, Un A, Ali M, et al. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. The Scientific World Journal. 2014;2014.
  54. Jeyapal S, Kona SR, Mullapudi SV, Putcha UK, Gurumurthy P, Ibrahim A. Substitution of linoleic acid with α-linolenic acid or long chain n-3 polyunsaturated fatty acid prevents Western diet induced nonalcoholic steatohepatitis. Scientific reports. 2018;8(1):1-14.
  55. Skulas-Ray AC, Kris-Etherton PM, Harris WS, Vanden Heuvel JP, Wagner PR, West SG. Dose-response effects of omega-3 fatty acids on triglycerides, inflammation, and endothelial function in healthy persons with moderate hypertriglyceridemia. The American journal of clinical nutrition. 2011;93(2):243-52.
  56. Hussein MA. Purslane extract effects on obesity-induced diabetic rats fed a high-fat diet. Malaysian journal of nutrition. 2010;16(3).
Medical Journal of Mashhad university of Medical Sciences
Volume 65, Issue 6
January and February 2023
Pages 2273-2284

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