Plasmid Replicon Typing of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal Children Using PCR Method Based on Plasmid Incompatibility Groups

Document Type : Research Paper


Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran


Introduction: Plasmids play a the crucial role in the horizontal transfer of genes, especially antibiotic resistance and pathogenic agents, and not epidemiological studies have been conducted on the type of plasmids transmitted by Enteroaggregative Escherichia coli pathotypes. The main purpose of this study is to classify the plasmids of Enteroaggregative Escherichia coli isolates isolated from children with diarrhea based on the major incompatible plasmid groups according method of Johnsnon et al(2007).
Materials and Method: This descriptive study was performed on 44 isolates of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal to identify 18 plasmid replicons by The method of Johnsnon et al(2007).
Results: The results showed the FreP 35(79.54%) plasmid replicon was the most frequent and L/M plasmid replicon was the least frequent with 1(2.27%). IncFIC, IncN, IncW, Inc X, Inc HI1, Inc HI2 and IncFIIA replicons were not observed in any of the isolates.
Conclusion: The results of this study indicated a high diversity of profile plasmid incompatibility groups among the studied isolates.


Main Subjects

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