Plasmid Replicon Typing of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal Children Using PCR Method Based on Plasmid Incompatibility Groups

Document Type : Research Paper

Authors

Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Introduction: Plasmids play a the crucial role in the horizontal transfer of genes, especially antibiotic resistance and pathogenic agents, and not epidemiological studies have been conducted on the type of plasmids transmitted by Enteroaggregative Escherichia coli pathotypes. The main purpose of this study is to classify the plasmids of Enteroaggregative Escherichia coli isolates isolated from children with diarrhea based on the major incompatible plasmid groups according method of Johnsnon et al(2007).
Materials and Method: This descriptive study was performed on 44 isolates of Enteroaggregative Escherichia coli Isolates Isolated from Diarrheal to identify 18 plasmid replicons by The method of Johnsnon et al(2007).
Results: The results showed the FreP 35(79.54%) plasmid replicon was the most frequent and L/M plasmid replicon was the least frequent with 1(2.27%). IncFIC, IncN, IncW, Inc X, Inc HI1, Inc HI2 and IncFIIA replicons were not observed in any of the isolates.
Conclusion: The results of this study indicated a high diversity of profile plasmid incompatibility groups among the studied isolates.

Keywords

Main Subjects

References

  1. Frost LS, Leplae R, Summers AO, Toussaint A. Mobile genetic elements: the agents of open source evolution. Nature Reviews Microbiology. 2005;3(9):722-32.
  2. Johnson TJ, Giddings CW, Horne SM, Gibbs PS, Wooley RE, Skyberg J, et al. Location of increased serum survival gene and selected virulence traits on a conjugative R plasmid in an avian Escherichia coli Avian Diseases. 2002;46(2):342-52.
  3. Smets BF, Barkay T. Horizontal gene transfer: perspectives at a crossroads of scientific disciplines. Nature Reviews Microbiology. 2005;3(9):675-8.
  4. Bertini A, Poirel L, Mugnier PD, Villa L, Nordmann P, Carattoli A. Characterization and PCR-based replicon typing of resistance plasmids in Acinetobacter baumannii. Antimicrob Agents Chemother. 2010; 54(10):4168-77.
  5. Hopkins KL, Liebana E, Villa L, Batchelor M, Threlfall EJ, Carattoli A. Replicon typing of plasmids carrying CTX- M or CMY beta-lactamases circulating among Salmonella and Escherichia coli Antimicrob Agents Chemother. 2006;50(9):3203
  6. Couturier M, Bex F, Bergquist PL, Maas WK. Identification and classification of bacterial plasmids. Microbiological Reviews. 1988;52(3):375-95.
  7. Johnson TJ, Wannemuehler YM, Johnson SJ, Logue CM, White DG, Doetkott C, et al. Plasmid replicon typing of commensal and pathogenic Escherichia coli Applied and Environmental Microbiology. 2007;73(6):1976-83.
  8. Boyd D, Cloeckaert A, Chaslus-Dancla E, Mulvey MR. Characterization of variant Salmonella genomic island 1 multidrug resistance regions from serovars Typhimurium DT104 and Agona. Antimicrob Agents Chemother. 2002;46(6):1714-22.
  9. Gilmour MW, Thomson NR, Sanders M, Parkhill J, Taylor DE. The complete nucleotide sequence of the resistance plasmid R478: defining the backbone components of incompatibility group H conjugative plasmids through comparative genomics. Plasmid. 2004;52(3):182-202.
  10. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. Journal of Microbiological Methods. 2005;63(3):219-28.
  1. Pierad D, De Greve H, Haesbrouk F, Mainil J. O157:H7 and O104:H4 Vero/Shiga toxin-producing Escherichia coli outbreaks respective role of cattle and humans. Veterinary Research. 2012;43:13.
  1. Estrada T, Navarro F. Enteroaggregative Escherichia coli pathotype a genetically heterogeneous emerging foodborne enteropatogen. FEMS Medical Microbiology and Immunology.2012;66:1–18.
  1. Mary A, Chikwelu L, Sandeep D, Kamaldeen AB. Molecular basis of virulencein clinical isolates of Escherichia coli and Salmonella species from at ertiary hospital in the Eastern Cape, South Africa. Gut Pathogens. 2011;3:3-9.
  2. Vilchez S, Reyes D, Paniagua M, Bucardo F, Mollby R, Weintrau A. Prevalence of diarrheagenic Escherichia coli in children from Leon ,Nicaragua. Journal of Medical Microbiology. 2009;58:630-637.
  3. Boschi-Pinto C, Velebit L, Shibuya K. Estimating child mortality due to diarrhea in developing countries. Bull World Health Organ. 2008;86:710-7.
  4. Kotloff KL, Blackwelder WC, Nasrin D, Nataro JP, Farag TH, Vaneijk A, et al. The Global Enteric Multicenter Study (GEMS) of diarrheal disease in infants and young children in developing countries epidemiologic and clinical methods of the case/control study. Clincal Infect Disease. 2012;55(l 4):232-245.
  5. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews. 1998; 11:142–201.
  6. Arenas MM, Martínez Y, Torres AG. Clinical implications of enteroadherent Escherichia coli. Current Gastroenterology Reports. 2012;14:386–394.
  7. Nataro JP, Kaper JB, Robins-Browne R, Prado V, Vial P, Levine MM. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatric Infectious Disease Journal.1987;6: 829-831.
  8. Wanke CA, Schorling JB, Barrett LJ, Desouza MA, Guerrant RL. Potential role of adherence traits of Escherichia coli in persistent diarrhea in an urban Brazilian slum.  Pediatric Infectious Disease Journal. 1991;10:746-751.
  9. Mathewson JJ, Jiang ZD, Zumla A. HEp2 cell-adherent Escherichia coli in patients with human immunodeficiency virus-associated diarrhea. Journal Infect Diseases. 1995;171: 1636-39.
  10. Cohen MB, Nataro JP,  Bernstein DI, Hawkins J, Roberts N, Staat MA.Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis a prospective controlled study. Journal Pediatr. 2005;146:54-61.
  11. Nataro JP, Mai V, Johnson J. Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut. Clinical Infectious Diseases. 2006;43: 402-407.
  12. Koo HL, Jiang ZD, Brown E, Garcia C, Qi H, Dupont HL. Coliform contamination of vegetables obtained from popular restaurants in Guadalajara, Mexico, and Houston, Texas. Clinical Infectious Diseases. 2008;47:218-221.
  13. Vigil KJ, Jiang ZD, Chen JJ. Coliform and Escherichia coli contamination of desserts served in public restaurants from Guadalajara, Mexico, and Houston, Texas. The American Journal of Tropical Medicine and Hygiene. 2009;80: 606-608.
  14. Rashki A, Keshtgar A, Hadadi F. Replicon Typing of O2, O6, and O157 Serotypes of Escherichia Coli Using PCR Method Based on Plasmid Incompatibility Groups. Journal of Babol University of Medical Sciences. 2018; 20(7): 40-6.
  15. Abraham S, Chapman TA, Zhang R, Chin J, Mabbett AN, Totsika M, et al. Molecular characterization of Escherichia coli strains that cause symptomatic and asymptomatic urinary tract infections. Journal Clinical Microbiology. 2012;50(3):1027-30.
  16. Xia L, Liu Y, Xia S, Kudinha T, Xiao SN, Zhong NS, et al. Prevalence of ST1193 clone and IncI1/ST16 plasmid in E. coli isolates carrying blaCTX-M-55 gene from urinary tract infections patients in China. Scientific Reports. 2017;7:44866.
  17. Cloeckaert A, Praud K, Doublet B, Bertini A, Carattoli A, Butaye P, et al. Dissemination of an extended-spectrum-beta-lactamase blaTEM-52 gene-carrying IncI1 plasmid in various Salmonella enterica serovars isolated from poultry and humans in Belgium and France between 2001 and 2005. Antimicrob Agents Chemother. 2007; 51(5): 1872-5.
  18. Cloeckaert A, Praud K, Lefevre M, Doublet B, Pardos M, Granier SA, et al. IncI1 plasmid carrying extended-spectrum-beta-lactamase gene blaCTX-M-1 in Salmonella enterica isolates from poultry and humans in France, 2003 to 2008. Antimicrob Agents Chemother.54(10):4484-6.
  19. Garcia-Fernandez A, Chiaretto G, Bertini A, Villa L, Fortini D, Ricci A, et al. Multilocus sequence typing of IncI1 plasmids carrying extended-spectrum beta-lactamases in Escherichia coli and Salmonella of human and animal origin. Journal Antimicrob Chemother. 2008;61(6):1229-33.
Medical Journal of Mashhad university of Medical Sciences
Volume 66, Issue 6
January and February 2024

Files

Share

How to cite

Statistics