Effect of Incorporation of CPP-ACP into an Iranian Toothpaste on Remineralization of Enamel Lesion

Document Type : Research Paper


1 School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

2 Dental Materials Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3 3Graduate student, School of Dentistry, University of Alberta, Edmonton, Alberta, Canada

4 Private Office, Mashhad, Iran

5 Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


The aim of this study is to examine the impact of incorporating Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP), produced at the Dental Materials Research Center of Mashhad University of Medical Sciences, into an Iranian toothpaste on the remineralization of enamel lesions.
Material and Method
Twenty enamel blocks were obtained by cutting sections from the crowns of cow incisor teeth. Each enamel block was divided into thirds; one-third was varnished to serve as the healthy control, while the remaining two-thirds underwent a demineralization process.
Following demineralization, the remaining half of the enamel block surface was varnished, leaving only the third area exposed to the remineralization process. The 20 enamel blocks were then divided into two groups: the first group received Meridant Complete7 toothpaste, and the second group received Meridant Complete7 toothpaste containing 1% CPP-ACP by weight.
In each group, the designated toothpaste was applied to the exposed third area for 3 minutes, followed by washing with distilled water and placement in an artificial saliva solution. This procedure was repeated daily for seven days. Remineralization was assessed using the Vickers microhardness method, measuring at depths of 20, 50, 100, and 150 micrometers in the three designated areas, from the surface to the depth.
The results indicated that the incorporation of CPP-ACP into Merident toothpaste significantly enhanced remineralization at depths of 20 micrometers (p=0.028) and 50 micrometers (p=0.033). However, at other depths, the addition of CPP-ACP did not yield a statistically significant difference in the toothpaste's impact on remineralization.
In conclusion, the findings of this study demonstrate that the incorporation of CPP-ACP into Merident Iranian toothpaste enhances its efficacy in remineralizing enamel lesions, particularly in the surface layer of tooth enamel


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