Document Type : Research Paper
Authors
1
PhD studentDepartment of Horticulture, College of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh,
2
Assistant Professor Department of Horticulture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
3
Assistant ProfessorDepartment of Veterinary medicine, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
4
Assistant Professor Department of Agronomy, College of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
Abstract
Eremostachys spp. have been considered as common herbal medicine in Iranian folk medicine, with pain relief medical applications. To investigate antinociceptive effects of Eremostachys laevigata, methanol, distilled water, chloroform, N-hexan and acetone extracts of root were extracted and injected to mice 30 minutes before pain induced by formalin, acid acetic injection and hot plate methods, in the concentrations of 100, 200, 300 and 400 mg/kg. The positive and negative controls were morphine and normal saline, respectively. Remarkable antinociceptive effect was observed in plant root methanol extract on the pain caused by formalin injection in both acute and chronic phases, visceral pain induced by acetic acid test, and increasing of pain tolerance duration in hot plate test. In hot plate and formalin test, the plant extract of 400 mg/kg could relief the pain as much as morphine, but not in acetic acid test, indicating that it may act through the central nervous than peripheral system. The phenolic content was determined using Folin-Ciocalteu assay. The total flavonoids were measured spectrophotometrically by the aluminium chloride colorimetric assay. The amount of total phenolic was 6.87 mg/g dry weight, expressed as gallic acid equivalents (GAE) and flavonoid was 0.19 mg/g dry weight expressed as rutin equivalents (RE). Two antipain iridoid glucosides, phloyoside I and Sesamoside have separated reversed-phase preparative HPLC from the root of this plant. Therefore, E. laevigata root methanol extract could be a suitable candidate for being considered as pain reliever.
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