Molecular Analyze of Virulence Genes in Enteroaggregative Escherichia coli (EAEC) Isolates Isolated from Diarrhea Children

Imanpanah, Zakiyeh; Hashemi Tabar, Gholamreza; Askari Badouei, Mahdi

doi:10.22038/mjms.2024.73759.4364

Molecular Analyze of Virulence Genes in Enteroaggregative Escherichia coli (EAEC) Isolates Isolated from Diarrhea Children

Document Type : Research Paper

Authors

zakiyeh imanpanah ¹

Gholamreza Hashemi Tabar ²

mahdi Askari Badouei ³

¹ microbiology ferdowsi university of mashhad mashhad iran

² 1. Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

10.22038/mjms.2024.73759.4364

Abstract

Introduction: Enteroaggregative Escherichia coli has many virulence genes that are related to its pathogenicity. The main purpose of this study was to the molecular analyze virulence genes of Enteroaggregative Escherichia coli isolates isolated from diarrhea children.
Materials and Method: In this cross-sectional descriptive study, after collecting 410 stool samples from diarrhea children, 352 Escherichia coli isolates were isolated using biochemical tests, and 39 EAEC isolates were isolated using the molecular PCR method and CVD432, aggR primers, and virulence genes were investigated. fimA, aap, astA, aafA, cdt in Enteroaggregative Escherichia coli isolates was performed by molecular PCR method.
Results: According to the results of the molecular method, out of a total of 39 EAEC isolates, respectively, the most isolates related to 37 (94.87%) fimA gene, 35 (89.74%) aap gene, 11 (20.28%) astA gene, 3(69 (7%) aafA gene and cdt gene were not found in any of the isolates.
Conclusion: The results showed that in EAEC isolates, the most frequent virulence genes are related to aap and fimA, and these genes play a role in bacterial aggregation and penetration into mucous tissue and intracellular accumulation of isolates, and in adhesion and biofilm formation. On the other hand, the presence of various pathogenic genes in EAEC isolates indicates the direct involvement of these genes in bacterial pathogenicity.

Keywords

“EAEC”

“virulence genes”

“Children's diarrhea”

“Molecular Analyze”

Subjects