Investigation of changes in some neurotrophins in hippocampal tissue in an experimental autoimmune encephalomyelitis model following endurance training and royal jelly

Document Type : Research Paper

Authors

1 Department of Exercise Physiology - Central Tehran Branch - Islamic Azad University - Tehran - Iran

2 Department of Exercise Physiology- Central Tehran Branch- Islamic Azad University- Tehran-Iran

10.22038/mjms.2025.88004.5008

Abstract

Introduction: Neurotrophin disorders are a common mechanism in multiple sclerosis. Given the essential role of neurotrophins in cognitive function, the present study aimed to investigate the changes in some neurotrophins in hippocampal tissue in an experimental autoimmune encephalomyelitis (EAE) model following endurance exercise (ET) and royal jelly (RJ).
Methods: In this experimental study, 49 female Sprague-Dawley rats with EAE (with complete Freund's adjuvant) were divided into groups: (1) EAE, (2) Sham (Sh), (3) RJ mg/kg 50 (RJ50), (4) RJ100, (5) ET, (6) ET+RJ50, (7) ET+RJ100. To investigate the effect of EAE on the variables, 7 healthy rats were placed in the healthy control (HC) group. ET was performed for five weeks, four sessions per week at a speed of 11-15 m/min for 30 minutes, and RJ was injected intraperitoneally at doses of (50 and 100 mg/kg/day). One-way ANOVA and Tukey's post hoc test were used to analyze the findings (P≥0.05).
Results: The results of the present study showed that BDNF levels in the ET group were significantly higher than in the EAE group. The results of the present study showed that BDNF levels in the RJ50, RJ100, ET+RJ50, and ER+RJ100 groups were significantly higher than in the EAE group. In addition, TrKB gene expression levels in the RJ100 group were significantly higher than in the RJ50 group. Also, TrKB levels in the RJ50, RJ100, ET+RJ50, and ER+RJ100 groups were significantly higher than in the EAE group.

Keywords

Main Subjects

References

  1. Haki, M., Al-Biati, H. A., Al-Tameemi, Z. S., Ali, I. S., & Al-Hussaniy, H. A. (2024). Review of multiple sclerosis: Epidemiology, etiology, pathophysiology, and treatment. Medicine, 103(8), e37297.‏
  2. Moghtaderi, A., Shahidi-Pourakbari, M., Izadi, S., Khosravi, A., & Hashemzehi, Z. (2023). Ongoing increase in incidence and prevalence of multiple sclerosis in south-eastern Iran: A three decade study. Multiple Sclerosis and Related Disorders, 71, 104557.‏
  3. Gich, J., Salavedra-Pont, J., Coll-Martinez, C., Quintana, E., Álvarez-Bravo, G., Robles-Cedeño, R., ... & Ramió-Torrentà, L. (2024). The nature of memory impairment in multiple sclerosis: understanding different patterns over the course of the disease. Frontiers in Psychology, 14, 1269794.‏
  4. Vacaras, V., Paraschiv, A. C., Iluț, S., Vacaras, C., Nistor, C., Marin, G. E., ... & Mureșanu, D. F. (2024). Brain-Derived Neurotrophic Factor in Multiple Sclerosis Disability: A Prospective Study. Brain Sciences, 14(3), 243.‏
  5. Nociti, V., & Romozzi, M. (2023). The role of BDNF in multiple sclerosis neuroinflammation. International Journal of Molecular Sciences, 24(9), 8447.‏
  6. Robinson, A. P., Harp, C. T., Noronha, A., & Miller, S. D. (2014). The experimental autoimmune encephalomyelitis (EAE) model of MS: utility for understanding disease pathophysiology and treatment. Handbook of clinical neurology, 122, 173-189.‏
  7. Ghanbari, P., Khajehzadeh, S., Sayyed, A., Raeisi, D., & Salehi, O. (2022). The effect of high intensity interval training with beetroot (Beta vulgaris) juice supplementation on serotonin and dopamine receptors expression, anxiety and depression in middle-aged diabetic rats. Avicenna Journal of Phytomedicine, 12(6), 627.‏
  8. Salehi, O. R., Sheikholeslami-Vatani, D., & Hosseini, S. A. (2021). Psycho-physiological effects of high intensity interval training and vitamin E consumption in elderly trimethyltin-treated Alzheimer's rats. Metabolism and Exercise, 11(2), 57-76.‏
  9. Hosseini, S. A., Salehi, O., Keikhosravi, F., Hassanpour, G., Ardakani, H. D., Farkhaie, F., ... & Azarbayjani, M. A. (2022). Mental health benefits of exercise and genistein in elderly rats. Experimental aging research, 48(1), 42-57.‏
  10. Salehi, O. R., Hosseini, S. A., Farkhaie, F., Farzanegi, P., & Zar, A. (2019). The effect of moderate intensity endurance training with genistein on brain-derived neurotrophic factor and tumor necrosis factor-α in diabetic rats. Journal of nutrition, fasting and health, 7(1), 44-51.‏
  11. Shobeiri, P., Karimi, A., Momtazmanesh, S., Teixeira, A. L., Teunissen, C. E., van Wegen, E. E., ... & Rezaei, N. (2022). Exercise-induced increase in blood-based brain-derived neurotrophic factor (BDNF) in people with multiple sclerosis: A systematic review and meta-analysis of exercise intervention trials. PloS one, 17(3), e0264557.‏
  12. Cheng, S. M., & Lee, S. D. (2022). Exercise training enhances BDNF/TrkB signaling pathway and inhibits apoptosis in diabetic cerebral cortex. International Journal of Molecular Sciences, 23(12), 6740.‏
  13. Seyed-Alhosseini, M., Rajabi, H., Ghadiri, A. A., Gharakhanlou, R., & Sarkaki, A. (2017). The Effect of Four Weeks of Aerobic Swimming on the Levels of Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Receptor Kinase B (TrkB) in the Brain of Animal Model of Experimental Autoimmune Encephalitis (EAE). Journal of Isfahan Medical School, 35(448), 1263-1270.‏
  14. Kawahata, I., Xu, H., Takahashi, M., Murata, K., Han, W., Yamaguchi, Y., ... & Yamakuni, T. (2018). Royal jelly coordinately enhances hippocampal neuronal expression of somatostatin and neprilysin genes conferring neuronal protection against toxic soluble amyloid-β oligomers implicated in Alzheimer’s disease pathogenesis. Journal of Functional Foods, 51, 28-38.‏
  15. Dehbozorgi, A., Tabrizi, L. B., Hosseini, S. A., & Rasoli, M. H. (2020). Effects of swimming training and royal jelly on BDNF and NGF gene expression in hippocampus tissue of rats with Alzheimer’s disease. Zahedan Journal of Research in Medical Sciences, 22(2).‏
  16. Tavakolian, S., Peeri, M., Fattahi Masrour, F., & Hajghasem, A. (2024). The Effect of Royal Jelly and Endurance Exercise on Cognitive Function and Pathological Changes of Hippocampus Tissue in Rats with Experimental Autoimmune Encephalomyelitis. Journal of Nutrition, Fasting and Health, 12(2), 90-98.‏
  17. Lohrasbi, M., Taghian, F., Dehkordi, K. J., & Hosseini, S. A. (2022). Interactive Effects of Endurance Training With Royal Jelly Consumption on Motor Balance in an Experimental Encephalomyelitis Model. Journal of Nutrition, Fasting & Health, 10(1).‏
  18. Kheirdeh, M., Jahromi, M. K., Hemmatinafar, M., & Nemati, J. (2023). Additive beneficial effects of aerobic training and royal jelly on hippocampal inflammation and function in experimental autoimmune encephalomyelitis rats. Multiple Sclerosis and Related Disorders, 70, 104527.‏
  19. Lohrasbi, M., Taghian, F., Jalali Dehkordi, K., & Hosseini, S. A. (2022). The functional mechanisms of synchronizing royal jelly consumption and physical activity on rat with multiple sclerosis-like behaviors hallmarks based on bioinformatics analysis, and experimental survey. BMC neuroscience, 23(1), 34.‏
  20. Mousavi, S., Fallahmohammadi, Z., & Hajizadeh Moghaddam, A. (2018). Evaluating the protective effect of 6 weeks resistance training and vitamin D intake on brain neuro-inflammatory factors in female rats with experimental autoimmune encephalomyelitis. Feyz Medical Sciences Journal, 22(6), 573-580.‏
  21. Malekinejad, H., Ahsan, S., Delkhosh-Kasmaie, F., Cheraghi, H., Rezaei-Golmisheh, A., & Janbaz-Acyabar, H. (2016). Cardioprotective effect of royal jelly on paclitaxel-induced cardio-toxicity in rats. Iranian journal of basic medical sciences, 19(2), 221.‏
  22. Tajiri, N., Yasuhara, T., Shingo, T., Kondo, A., Yuan, W., Kadota, T., ... & Date, I. (2010). Exercise exerts neuroprotective effects on Parkinson's disease model of rats. Brain research, 1310, 200-207.‏
  23. Bernardes, D., & Oliveira, A. L. R. D. (2018). Regular exercise modifies histopathological outcomes of pharmacological treatment in experimental autoimmune encephalomyelitis. Frontiers in neurology, 9, 950.‏
  24. Taherian, N., Vaezi, G., Neamati, A., Hojjati, V., Ghorbani-Taherdehi, F., Sahebkar, A., & Gorji-Valokola, M. (2024). The dose-dependent neuroprotective effect of norepinephrine in improving memory retrieval in an experimental model of multiple sclerosis, experimental autoimmune encephalomyelitis. Brain research bulletin, 209, 110907.‏
  25. Hamdi, L., Nabat, H., Goldberg, Y., Fainstein, N., Segal, S., Mediouni, E., ... & Einstein, O. (2022). Exercise training alters autoimmune cell invasion into the brain in autoimmune encephalomyelitis. Annals of Clinical and Translational Neurology, 9(11), 1792-1806.‏
  26. Cefis, M., Chaney, R., Wirtz, J., Méloux, A., Quirié, A., Leger, C., ... & Garnier, P. (2023). Molecular mechanisms underlying physical exercise-induced brain BDNF overproduction. Frontiers in molecular neuroscience, 16, 1275924.‏
  27. Karimi, N., Ashourizadeh, H., Pasha, B. A., Haghshomar, M., Jouzdani, T., Shobeiri, P., ... & Rezaei, N. (2022). Blood levels of brain-derived neurotrophic factor (BDNF) in people with multiple sclerosis (MS): A systematic review and meta-analysis. Multiple Sclerosis and Related Disorders, 65, 103984.‏
  28. Giti, Z., Banaeifar, A., Arshadi, S., & Azarbayjani, M. A. (2021). Effect of Eight Weeks of Positive Slope and Negative Slope Training, Along with Royal Jelly on The Hippocampal Expression Of Β-Amyloid And Γ-Secretase in TrimethyltinInduced Alzheimer's Disease Rats. Journal of Nutrition, Fasting & Health, 9(1).‏
  29. Ziaee Bashirzad, M., Cheragh-Birjandi, S., Younessi HERAVI, M. A., & Salarinia, R. (2024). The effect of four weeks of different aerobic exercises on the gene expression of tropomyosin kinase B receptor and phosphatidyl-inositol-3-kinase in hippocampal rats with spinal cord injury. Journal of Practical Studies of Biosciences in Sport.‏
  30. Hosseini, A., PARNOW, A. H., Karimi, I., & HOSSEINI, B. (2016). Effect of 4-week resistance training on plasma brain derived neurotrophic factor in rats.‏
  31. Ashofteh, A., CHERAGH, B. S., & TaheriChadorneshin, H. (2022). The Effect of Resistance Training Along with Royal Jelly Supplementation on Genes Expression of ProNGF and P75 Receptor in the Hippocampal Tissue of Alzheimer's Rats.‏
  32. Mosalla nezhad A, Taghian F, Jalali Dehkordi K, Hosseini S A. The Effect of Aerobic Training with Royal Jelly Consumption on Health Related Anthropometric Markers in an Experimental Autoimmune Encephalomyelitis Model. Jorjani Biomed J 2021; 9 (4) :1-12
Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 3
May and June 2025
Pages 644-656

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