Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

The effect of 6 weeks of treadmill training combined with genistein consumption on iNOS and Cdc2 gene expression in BalbC mice with breast cancer

Document Type : Research Paper

Authors
Seyadeh Zahra Hajiaghaei 1
Sanaz Mirzayan Shanjani 2
Zohreh Afsharmand 1
Yaser Kazemzadeh 1
1 Department of Exercsie Physiology, Isl.C., Islamic Azad University, Islamshahr, Iran
2 Department of Exercsie Physiology, Isl.C., Islamic Azad University, Islamshahr, Iran (Corresponding Author)
10.22038/mjms.2025.27393
Abstract
Introduction: The aim of the study was to investigate the effect of 6 weeks of treadmill exercise combined with genistein on iNOS and Cdc2 gene expression in Balb/C mice with breast cancer.
 Methods: In this basic, experimental study, which was conducted on 40 female Balb/C mice, after induction of breast cancer, the mice were randomly divided into 4 groups of 10: cancer mice, cancer mice with a treadmill exercise period, cancer mice with genistein supplementation, and cancer mice with a treadmill exercise period and genistein supplementation. Oral gavage of genistein supplement between 100 and 150 microliters was performed, 5 days a week, for six weeks. Treadmill exercise was performed at a speed of 20 meters per minute, 0% incline for 30 minutes per day, 5 times a week for 6 weeks. Two-way analysis of variance with repeated measures was used to analyze the data.
Results: The results showed that the main effect of treadmill exercise and the main effect of genistein supplementation on gene expression in female Balb/C mice after induction of breast cancer were statistically significant (P < 0.05).
Conclusion: Exercise and genistein intake effectively prevent cancer progression by reducing iNOS and CDC-2 gene expression in Balb/C mice induced with breast cancer.
Keywords
Aerobic training
genistein supplementation
iNOS
Cdc2
Balb/c mice
breast cancer

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Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 5
November and December 2025
Pages 1595-1603