The effect of endurance training, high intensity interval training and Medicago Sativa extract on the mir126 in breast cancer rats

Takhti, Saeid; Banaeifar, Abdolali; Eizadi, Mojtaba; Arshadi, Sajad; Tabatabaey, Hamid

doi:10.22038/mjms.2025.27691

The effect of endurance training, high intensity interval training and Medicago Sativa extract on the mir126 in breast cancer rats

Document Type : Research Paper

Authors

Saeid Takhti ¹

Abdolali Banaeifar ²

Mojtaba Eizadi ³

Sajad Arshadi ²

Hamid Tabatabaey ⁴

¹ PhD student in Exercise Physiology, ST.C., Islamic Azad University, Tehran, Iran.

² Associate Professor, Department of Exercise Physiology, ST.C., Islamic Azad University, Tehran, Iran.

³ Assistant Professor, Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Markazi, Iran.

⁴ Assistant Professor, Department of Exercise Physiology, ST.C.,, Islamic Azad University Tehran, Iran.

10.22038/mjms.2025.27691

Abstract

Introduction: Breast cancer is the most common cancer and the leading cause of cancer-related death among women. In 2018, about ½ million people were diagnosed with breast cancer worldwide, and about one in four women had breast cancer. Exercise training can have different effects on cancer control by affecting microRNAs.
Methods: The statistical population of the present study consisted of all female Balb C mice of the Laboratory Animal Center of Shiraz University of Medical Sciences, which were purchased from a statistical population of 90 10-week-old female Balb C mice weighing 17±2 grams. An aerobic, interval, and resistance training program was performed for 12 weeks, 5 sessions per week, with a gradual increase in intensity and duration. Shapiro-Wilk, Levine, one-way analysis of variance, and Tukey post hoc tests were used to analyze the data. Daily consumption of 0.1 ml of alfalfa extract was done via intraperitoneal injection for 12 weeks.
Results: The results showed that the level of mir126 was increased in all training groups with and without alfalfa extract consumption, but this level was more significant in the endurance training groups with and without alfalfa extract consumption than in the other groups.
Conclusion: The present study showed that exercise training combined with alfalfa consumption can play a very important role in modulating and controlling breast cancer conditions by increasing microRNAs.

Keywords

Endurance training

interval training

resistance training

breast cancer

microRNA