Medical Journal of Mashhad university of Medical Sciences

Medical Journal of Mashhad university of Medical Sciences

Frequency of Klebsiella Pneumoniae Producing Carbapenemase KPC in Clinical specimens of Mashhad during 2014

Document Type : Research Paper

Authors
Fateme roudbari 1
Kiarash Ghazvini 2
Somayeh heydari fork 3
Mahdi KouhiNoghondar 4
Saeed Amel Jamedar 2
Masoud Youssefi 2
1 Department of cell and biology, Faculty of science,University of Mazandaran, Babolsar, Iran
2 Antimicrobial resistance Research Center, Buali research institute, Mashhad University of medical Sciences, Mashhad, Iran Department of Microbiology and Virology, School of medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of cell and biology, Faculty of science,University of Mazandaran, Babolsar, Iran.
4 MSc Antimicrobial resistance Research Center, Buali research institute, Mashhad University of medical Sciences, Mashhad, Iran
10.22038/mjms.2015.5059
Abstract
Introduction:
Klebsiella pneumoniae producing B-Lactamase is an important medical concern. Therefore we aimed to investigate broad spectrum lactamase KPC-producing Klebsiella pneumoniae in clinical samples in Mashhad during 2014.
 
Material and method
After identification by biochemical methods, antibiotic susceptibility of Klebsiella pneumoniae isolates against 10 different antibiotics was identified using disk diffusion technique. To evaluate the presence of carbapenemase  KPC enzyme, modified Hodge test (MHT) was performed and the minimum inhibitory concentration (MIC) of Meropenem as an indicator of Carbapenem  class of β-Lactam antibiotics was determined using agar dilution test.
 
Results
Among the isolated bacteria, high level of resistance against cefazolin (54.44%), cefepime (49.46%), ceftazidime (45.19%), trimethoprim-sulfamethoxazole (46.26%) was observed. Modified Hodge test was performed for all 136 ESBL Klebsiella pneumoniaisolates and 20 were found to be positive for MHT. Furthered MIC test revealed that 2 isolate had MIC=4 μg/ml, 3 isolate had MIC=2, and remaining 15 isolates had MIC more than 8.
 
Conclusion
This study underscores the risk of increasing carbapenem resistant Klebsiella pneumonia specieswhich demands further attention in clinical settings.
Keywords
Klebsiella pneumonia
Carbapenemase
clinical isolates
Medical Journal of Mashhad university of Medical Sciences
Volume 58, Issue 6 - Serial Number 6
September 2015
Pages 322-329