Effects of knotweet or polygonum aviculare herbal extract on proliferation of HeLa cell line


1 MSc in Virology Tabriz university of Medical sciences, Tabriz, Iran

2 Professor of Anatomical sciences, Tabriz University of Medical sciences, Tabriz, Iran

3 Associate professor of Medicine Biotechnology research center, Iranian Blood Transfusion organization, Tehran, Iran

4 Assistant Professor of Biochemistry, Tabriz University of Medical sciences, Tabriz, Iran

5 MSc in Anatomical sciences, Tabriz University of Medical sciences, Tabriz, Iran

6 Assistant Professor of Anatomical sciences, Tabriz University of Medical sciences, Tabriz, Iran


Cervical cancer is the most common cancer in women living in developing countries. Recently, for treatment of diseases such as cancer, herbal medicine is used as a supplementary.The Aim of this study was assessment of anticancerous effects of polygonum aviculare herbal extract on Hela cervical cancer cell line
Materials and Methods
HeLa cells were cultured in RPMI -1640 with 10% Fetal Bovine Serum in 5% Co2 and at 37ºC in different concentrations (0, 0.005, 0.05, 0.01, 0.025, 0.075, 0.1, 0.125, 0.15, 0.175, 0.2, 0.25, 0.3, 0.35, 0.5, 5 mg/ml) of polygonum aviculare. For assessment of viability of cells, trypan blue staining was performed. MTT assay was used for proliferation detection.
Results: our results showed that in 0.15, 0.20 and 0.35 mg/ml proliferation of HeLa cells decreased according to MTT assay.
It was proved that polygonum aviculare had antioxidant component and could be a scavenger of free radical. Because of high production of free radicals in diseases such as cancer, the use of the herbal medicine with high amount of antioxidant could be a supplementary treatment in cancer and other diseases.


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