Investigation of the K1 and K2 capsular serotypes prevalence of Klebsiella pneumonia isolated from Urinary tracts and wounds infections

Document Type : Research Paper

Authors

1 Associate Professor of Molecular Genetics, Department of Biology, Sanandaj branch, Islamic Azad University, Sanandaj, Iran

2 Assistant Professor of Bacteriology, Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran

3 MSc of Microbiology, Department of Biology, Sanandaj branch, Islamic Azad University, Sanandaj, Iran

Abstract

Abstract
Introduction: Klebsiella pneumonia, a gram-negative bacterium, is one of the main causes of nosocomial infections. The purpose of this study is to determine K1 and K2 capsule serotypes in samples collected from hospitals in Kermanshah.
Materials & methods: This study was a Cross-Sectional – Descriptive research. The Samples were collected of patients with urinary tract infections or wounds caused by burns between the first 4 months of 2016. The serotype of samples was determined by using of PCR method for wzy and magA genes belong to CPS cluster which are necessary for biosynthesis of polysaccharide capsule K1 and K2, respectively, after approving bacteria by phenotypic method. 
Results: The findings showed that out of 60 samples approved in phenotypic studies with a 1: 1 ratio of urine and wound samples, 37 samples (61.67%) was belonged to K1 and 23 samples (38.33%) to K2 serotype. Also, among the urine samples of patients, 22 samples (73.33%) had K1 serotype and about of the samples belonging to wounds caused by burns, 21(70%) and 9(30%) samples had K1 and K2 serotype, respectively.

Keywords

References

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Medical Journal of Mashhad university of Medical Sciences
Volume 63, Issue 2
July and August 2020
Pages 2427-2435

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