Effect of 12-week resistance training on Mafa expression in pancreas tissue and serum insulin in type 2 diabetic rats

Document Type : Research Paper

Authors

1 Department of Physical Education and Sport Sciences, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran

2 Assistant Professor, Department of Physical Education and Sport Sciences, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran

Abstract

Abstract
Introduction: Increased Mafa expression with increased insulin secretion from pancreatic beta cells and consequent improvement in glycemic profile in type 2 diabetic patients. This study aimed to determine the effect of resistance on Mafa expression in pancreas tissue, glucose and insulin in type 2 diabetes rats.
Material and Methods: The statistical population consisted of all male Wistar rats of the pastor inistitue of Iran, among which 14 were male rat 10 weeks old weighting 220 ± 20 g. Type 2 diabetes induced by Intraperitoneal injection of nicotinamid + STZ. Then rats divided randomly into resistance (6 weeks, 5 times/weekly, n=7) and control (n=7) groups. Fasting glucose, serum insulin and Mafa expression in Pancreas tissue of both groups were measured after lasted exercise and compared between 2 groups by independent T test.
Results: Resistance training significantly reduced fasting glucose and significantly increased serum insulin levels compared with the control group. Resistance training also increased Mafa expression in pancreatic tissue( p>0/0001).
Conclusion: Based on these data, increased serum insulin in response to resistance training in T2D rats may be attributed with increased Mafa expression in pancreas tissue.

Keywords

References

  1. Adeghate E, Schattner P, Dunn E. An update on the etiology and epidemiology of diabetes mellitus.
    Ann NY Acad Sci. 2006; 1084, 1–29.
  2. 2. Melzer D, Murray A, Hurst AJ. Effects of the diabetes linked TCF7L2 polymorphism in a
    representative older population. BMC Med 2006 ; 4:34.
    3. Lyssenko V, Lupi R, Marchetti P. Mechanisms by which common variants in the TCF7L2 gene
    increase risk of type 2 diabetes. J Clin Invest 2007; 117:2155–2163.
    4. Villareal DT, Robertson H, Bell GI. TCF7L2 variant rs7903146 affects the risk of type 2 diabetes by
    modulating incretin action. Diabetes 2010, 59:479–485.
    5. Kitamura YI, Kitamura T, Kruse JP, Raum JC, Stein R, Gu W, Accili D. FoxO1 protects against
    pancreatic B-cell failure through NeuroD and MafA induction. Cell Metab. 2005; Sep; 2(3): 153-63.
    6. Matsuoka TA, Artner I, Henderson E, Means A, Sander M, and Stein R. The MafA transcription
    factor appears to be responsible for tis- sue-specific expression of insulin. Proc. Natl. Acad. Sci.
    USA.2004. 101: 2930– 2933.
    7. Olbrot, M., Rud, J., Moss, L.G., and Sharma, A. Identification of beta-cell-specific insulin gene
    transcription factor RIPE3b1 as mammalian MafA. Proc. Natl. Acad. Sci. USA. 2002; 99: 6737–
    6742.
    8. Kataoka K. MafA is a glucose-regulated and pancreatic beta-cell-specific transcriptional activator
    for the insulin gene. J Biol Chem. 2002; 277:49903–49910.
    9. Matsuoka TA. Members of the large Mafa transcription family regulate insulin gene transcription in
    islet beta cells. Mol Cell Biol. 2003; 23:6049-6062.
    10. Herrera Uribe J, Vitger AD, Ritz C, Fredholm M, Bjørnvad CR, Cirera S. Physical training and
    weight loss in dogs lead to transcriptional changes in genes involved in the glucose-transport
    pathway in muscle and adipose tissues. Vet J. 2016; Feb;208:227.
    11. Calegari VC, Abrantes JL, Silveira LR, Paula FM, Costa JM Jr., Rafacho A, et al. Endurance
    training stimulates growth and survival pathways and the redox balance in rat pancreatic islets. J
    Appl Physiol 2012; 112:711- 8.
    12. Zoppi CC, Calegari VC, Silveira LR, Carneiro EM, Boschero AC. Exercise training enhances rat
    pancreatic islets anaplerotic enzymes content despite reduced insu- lin secretion. Eur J Appl Physiol
    2011; 111:2369-74.
    13. Eizadi M, Soory R, Ravasi A, Baesy K, Choobineh S. Relationship between TCF7L2 Relative
    Expression in Pancreas Tissue with Changes in Insulin by High Intensity Interval Training (HIIT) in
    Type 2 Diabetes Rats . JSSU. 2017; 24 (12) :981-993.
    14. Eizadi M, Ravasi AA, Soory R, Baesi K, Choobineh S. The Effect of Three Months of Resistance
    Training on TCF7L2 Expression in Pancreas Tissues of Type 2 Diabetic Rats. Avicenna J Med
    Biochem. 2016 June; 4(1):e34014..
    15. Eizadi M, Ravasi AA, Soori R, Baesi K, Choubineh S. Effect of three months aerobic training on
    TCF7L2 expression in pancreatic tissue in type 2 diabet es rats induced by streptozotocinnicotinamide. Feyz 2017; 21(1): 1-8.
    16. Ramazani Rad M, Hajirasouli M, Eizadi M. The Effect of 12 Weeks of Aerobic Training on GLP-1
    Receptor Expression in Pancreatic Tissue and Glycemic Control in Type 2 Diabetic Rats. Qom Univ
    Med Sci J . 2017; 11 (6) :36-45.
    17. Rashidi M, Soori R, Choobineh S, Ravasi AA, Baesi K. The Effect of an Aerobic Exercise on
    MTNR1B Gene Expression, Insulin and Glucose Levels in Pancreas of Induced Diabetic Rat with
    Streptozotocin-Nicotinamide. Journal of Knowledge & Health 2016; 11(3): 40-48.
    18. Sunmin P, Sang MH, Ji EL, So RS. Exercise improves glucose homeostasis that has been impaired
    by a high-fat diet by potentiating pancreatic β-cell function and mass through IRS2 in diabetic rats.
    Journal of Applied Physiology November 2007; 103(5): 1764-1771.
  3. 19. Kaneto H, Matsuoka TA. Role of pancreatic transcription factors in maintenance of mature β-cell
    function. Int J Mol Sci. 2015 Mar 18;16(3):6281-97.
    20. Matsuoka, T. A., Kaneto, H., Stein, R., Miyatsuka, T., Kawamori, D., Henderson, E., Kojima, I.,
    Matsuhisa, M., Hori, M. and Yamasaki, Y.MafA regulates expression of genes important to islet
    β-cell function. Mol. Endocrinol.2007; 21 , 2764–2774.
    21. Song, Y. D., Lee, E. J., Yashar, P., Pfaff, L. E., Kim, S. Y. and Jameson, J. L.Islet cell differentiation
    in liver by combinatorial expression of transcription factors neurogenin-3, BETA2, and RIPE3b1.
    Biochem. Biophys. Res. Commun. . 2007; 354 , 334–339.
    22. Zhang C. , Moriguchi T. , Kajihara M,et al., “MafA is a key regulator of glucose-stimulated insulin
    secretion,” Molecular and Cellular Biology, , 2005; vol. 25, no. 12, pp. 4969–4976.
    23. Andrali. S. S., Smapley M. L., Vanderford N. L., and Ozcan S., “Glucose regulation of insulin gene
    expression in pancreatic B- cells,” Biochemical Journal, 2008; vol. 415, no. 1, pp. 1–1,.
    24. Harmon, J. S., Stein, R., & Robertson, R. P. Oxidative stress-mediated, post-translational loss of
    MafA protein as a contributing mechanism to loss of insulin gene expression in glucotoxic beta
    cells. The Journal of Biological Chemistry, 2005; 280(12), 11107-11113.
    25. Robertson, R. P., & Harmon, J. S.Diabetes, glucose toxicity, and oxidative stress: A case of double
    jeopardy for the pancreatic islet beta cell. Free Radical Biology & Medicine. 2006. 41(2), 177-
    184.
    26. Ligtenberg PC, Hoekstra JB, Bol E, Zonderland ML, Erkelens DW. Effects of physical training on
    metabolic control in elderly type 2 diabetes mellitus patients. Clin Sci (Lond). 1997 Aug; 93(2):127-
    35.
    27. Maltais ML, Perreault K, Courchesne-Loyer A, Lagacé JC, Barsalani R, Dionne IJ. Effect of
    Resistance Training and Various Sources of Protein Supplementation on Body Fat Mass and
    Metabolic Profile in Sarcopenic Overweight Older Adult Men: A Pilot Study. Int J Sport Nutr Exerc
    Metab. 2016 Feb; 26(1):71-7.
    28. Sheu WH, Chang TM, Lee WJ, Ou HC, Wu CM, Tseng LN, Lang HF, Wu CS, Wan CJ, Lee IT.
    Effect of weight loss on proinflammatory state of mononuclear cells in obese women. Obesity
    (Silver Spring). 2008 May;16(5):1033-8
medical journal of mashhad university of medical sciences
Volume 63, Issue 1
May and June 2020
Pages 2267-2276

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