Effects of a L-citrulline supplementation on nitric oxide and blood lactate in young women after a maximum exercise session

Document Type : Research Paper

Authors

1 Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran

2 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran

3 Assistant Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran

Abstract

Introduction: The consumption of sports supplements and their effects on the performance of athletes needs to be evaluated. The purpose of this study was to determine the effects of L-citrulline supplementation on nitric oxide (NO) and blood lactate in young women after a maximal exercise session.
Methods & Materials: The research method was quasi-experimental with double blind design. After filling out the health questionnaire, 24 voluntary inactive young women with body mass index less than 25 kg/m2 were randomly divided into two groups (n=12) and placebo (n=12). Subjects visited the laboratory twice; in the first session, the first blood samples were taken immediately after the exercise protocol. After 5 minutes of warm-up, the subjects were performed the Bruce treadmill test protocol to time to exhaustion. Then the supplement group consumed 6 grams daily L-citrulline powder and placebo (starch powder) for one week. The second session was performed one week later in the same conditions as the first session. Immediately after the test, time to exhaustion was recorded, blood samples collected and lactate and NO plasma levels measured. Analysis of variance with repeated measures with significant level p < 0.05 was used for analyzing data.
Findings: The results showed that there was no significant difference between time to exhaustion, plasma levels of lactate and NO of two groups (placebo and supplement) in two sessions.
Discussion & Conclusions: It seems that acute supplementation of L-citrulline has no effect on nitric NO and blood lactate in young women after a maximal exercise session.

Keywords

References

1- Wilkinson DJ, Smeeton NJ, Watt PW. Ammonia metabolism, the brain and fatigue;
revisiting the link. Progress in Neurobiology. 2010; 91(3):200-19.
2- Pitcher JB, Miles TS. Pitcher JB, Miles TS. Influence of muscle blood flow on fatigue during
intermittent human hand‐grip exercise and recovery. Clinical and Experimental
Pharmacology and Physiology. 1997; 24(7):471-6.
3- McConell GK. Effects of L-arginine supplementation on exercise metabolism. Current
Opinion in Clinical Nutrition & Metabolic Care. 2007; 10(1):46-51.
4- Alvares TS, Conte-Junior CA, Silva JT, Paschoalin VM. Acute L-arginine supplementation
does not increase nitric oxide production in healthy subjects. Nutrition & Metabolism. 2012;
9(1):1-8.
5- Kreider RB, Wilborn CD, Taylor L, Campbell B, Almada AL, Collins R, et al. ISSN exercise
& sport nutrition review: research & recommendations. Journal of the International Society
of Sports Nutrition. 2010; 7(1):1-43.
6- Fitts RH, Balog EM. Effect of intracellular and extracellular ion changes on E–C coupling
and skeletal muscle fatigue. Acta Physiologica Scandinavica. 1996; 156(3):169-81.
7- Rougé C, Des Robert C, Robins A, Le Bacquer O, Volteau C, De La Cochetière MF,
Darmaun D. Manipulation of citrulline availability in humans. American Journal of
Physiology-Gastrointestinal and Liver Physiology. 2007; 293(5):G1061-7.
8- Bendahan D, Mattei JP, Ghattas B, Confort-Gouny S, Le Guern ME, Cozzone PJ.
Citrulline/malate promotes aerobic energy production in human exercising muscle. British
Journal of Sports Medicine. 2002; 36(4):282-9.
9- Pérez-Guisado J, Jakeman PM. Citrulline malate enhances athletic anaerobic performance
and relieves muscle soreness. Journal of Strength and Conditioning Research. 2010;
24(5):1215-22.
10- Ashley J, Kim Y, Gonzales JU. Impact of L-citrulline supplementation on oxygen uptake
kinetics during walking. Applied Physiology, Nutrition, and Metabolism. 2018; 43(6):631-
37.
11- Moazami M, Taghizadeh V, Ketabdar A, Dehbashi M, Jalilpour R. Effects of oral L-arginine
supplementation for a week, on changes in respiratory gases and blood lactate in female
handballists. Iranian Journal of Nutrition Sciences & Food Technology. 2015; 9(4):45-52.
(Persian)
12- Yavuz HU, Turnagol H, Demirel AH. Pre-exercise arginine supplementation increases time
to exhaustion in elite male wrestlers. Biology of Sport. 2014; 31(3):187-91.
13- Bailey S. Acute L-arginine supplementation reduces the O2 cost of moderate-intensity
exercise and enhances high-intensity exercise tolerance. Journal of Applied Physiology.
2010; 109(5):1394-403.
14- Amorena CE, Wilding TJ, Manchester JK, Roos A. Changes in intracellular pH caused by
high K in normal and acidified frog muscle. Relation to metabolic changes. Journal of
General Physiology. 1990; 96(5):959-72
15- Wax B, Kavazis AN, Luckett W. Effects of supplemental citrulline-malate ingestion on
blood lactate, cardiovascular dynamics, and resistance exercise performance in trained males.
Journal of Dietary Supplements. 2016; 13(3):269-82.
16- Takeda K, Machida M, Kohara A, Omi N, Takemasa T. Effects of citrulline supplementation
on fatigue and exercise performance in mice. Journal of Nutritional Science and
Vitaminology (Tokyo). 2011; 57(3):246-50.
17- Santos Baião DD, Conte Jr CA, Joab Silva T, Flosi VM Paschoalin. L-arginine
supplementation and nitric oxide production: no additional effect when associated to
exercise. Food and Nutrition Sciences. 2013, 4(8):779-84.
18- Churchward-Venne TA, Cotie LM, MacDonald MJ, Mitchell CJ, Prior T, Baker SK, Phillips
SM. Citrulline does not enhance blood flow, microvascular circulation, or myofibrillar
protein synthesis in elderly men at rest or following exercise. American Journal of
Physiology-Endocrinology and Metabolism. 2014; 307(1):E71-83.
19- Liu TH, Hung W, Tsong-Rong J, Chang CK. Effect of short-term arginine supplementation
on vasodilation and performance in intermittent exercise in judo athletes. International
Journal of Sport and Exercise Science. 2010; 1(2):7-12.
20- Koppo K, Taes YE, Pottier A, Boone J, Bouckaert J, Derave W. Dietary arginine
supplementation speeds pulmonary VO2 kinetics during cycle exercise. Medicine & Science
in Sports & Exercise. 2009; 41(8):1626-32.
21- Hickner RC, Tanner CJ, Evans CA, Clark PD, Haddock A, Fortune C, et al. L-citrulline
reduces time to exhaustion and insulin response to a graded exercise test. Medicine &
Science in Sports & Exercise. 2006; 38(4):660-6.
22- Piatti P, Fragasso G, Monti LD, Setola E, Lucotti P, Fermo I, et al. Acute intravenous Larginine infusion decreases endothelin-1 levels and improves endothelial function in patients
with angina pectoris and normal coronary arteriograms: correlation with asymmetric
dimethylarginine levels. Circulation 2003; 107(3):429-36.
23- Koifman B, Wollman Y, Bogomolny N, Chernichowsky T, Finkelstein A, Peer G, et al.
Improvement of cardiac performance by intravenous infusion of L-arginine in patients with
moderate congestive heart failure. Journal of the American College of Cardiology. 1995;
26(5):1251-6.
24- Sureda A, Cordova A, Ferrer MD, Tauler P, Perez G, Tur JA, et al. Effects of L-citrulline
oral supplementation on polymorphonuclear neutrophils oxidative burst and nitric oxide
production after exercise. Free Radical Research. 2009; 43(9):828-35.
25- Hickner RC, Fisher JS, Ehsani AA, Kohrt WM. Role of nitric oxide in skeletal muscle blood
flow at rest and during dynamic exercise in humans. American Journal of Physiology. 1997;
273(1 Pt 2):H405-10.
medical journal of mashhad university of medical sciences
Volume 63, Issue 4 - Serial Number 4
November and December 2020
Pages 2509-2517

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