Effect of High Intensity Interval Training and Thyme Honey on Hepatic miR-423-5P, FAM3A, Akt2 Pathway of Type ll Diabetic rats

Document Type : Research Paper

Authors

1 Teacher of district one of education in Shiraz

2 Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, Islamic Azad University, South Tehran Branch, Tehran. Iran.

3 Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction: Micro-RNAs are effective in inflicting diabetes type II and its side effects. The Purpose of this study was to evaluate the effect of High Intensity Interval Training & thyme honey on expression of the genes of the hepatic miR-423-5P - FAM3A-AKt2 pathway in rats.
Materials and Methods: 36 male rats were fed with high-fat diets for 20weeks and were then injected streptozotocin (25 mg/kg) in order to inflict diabetes type II. The 36rats were then divided into four groups: Control, regular exercise group, nutritional intervention group and exercise with nutritional intervention group. Regular exercise included five sessions a week for eight weeks and nutritional intervention was done at a dosage of 3 g/kg of thyme honey using a gauge method. The amount of expression of miR-423-5P, mRNA FAM3A and mRNA AKt2 genes of the liver were assessed using RT-PCR and evaluation kits. The obtained findings were analyzed using the two-way analysis of variance and Bonferroni post hoc test.
Results: Expression of the miR-423-5P gene was significantly lower in all groups compared to control, expression of mRNA FAM3 had insignificant increase and expression of mRNA AKt2 had a significant increase in groups of regular exercise and exercise with nutritional intervention. This gene had an insignificant increase of expression in the group with only nutritional intervention. (p value<0.05)
Discussion: It seems that HIIT & consumption of thyme honey, both alone and interactively with greater effectiveness, can change in expression of the mentioned gene in liver of rats with diabetes type II.

Keywords

References

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medical journal of mashhad university of medical sciences
Volume 65, Issue 1
May and June 2022
Pages 101-115

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