Investigating the anticancer effect of the lantibiotic, Lacticin 3147

Document Type : Research Paper

Authors

1 Ph.D Student of Microbiology,Department of Biology,Damghan Branch, Islamic Azad University, Damghan, Iran

2 Associate Professor of Biochemistry ,Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

3 Assistant Professor of Mycology, Department of Biology, Damghan Branch,Islamic Azad University,Damghan ,Iran.

4 Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.

Abstract

The most common type of cancer is skin cancer, especially in men. Lacticin 3147 is a bacteriocin from the lantibiotic family. Since it is similar to nisin, Lacticin was considered for its anticancer properties’ investigation. The aim was to investigate the effect of Lacticin 3147. The MTT Assay helped assess A431 cell survival and cytotoxicity following treatment with Lacticin 3147 after 24 and 48 hours. The cell apoptosis amounts were investigated by measuring the activity of caspases 8 and 9 in A431 cancer cells treated with the IC50 concentration of Lacticin 3147 after 24, 48, and 72 hours. The Boyden Chamber method evaluated the invasion ability of A431 cancer cells treated with a sublethal dose of Lacticin 3147. Specific primers were designed for proapoptotic genes bax, antiapoptotic bcl-2, and the reference gene ꞵ-actin. The expression level in A431 cells treated with IC50 concentration of Lacticin 3147 was investigated using the quantitative Real-Time PCR method. Data were analyzed by SPSS 16 software and ANOVA statistical test (P<0.05).
Statistical analysis of the data obtained from the MTT test showed that the growth of cells treated with different concentrations of Lacticin 3147 decreased significantly (p<0.001). The inhibitory effect of Lacticin 3147 on the viability of A431 cells depends on the concentration and treatment time. As a result, a 0.3 µM concentration and 72-hour duration were the most consequential effects. Lacticin IC50 concentration was equal to 0.8 µM. The activity of caspases 8 and 9 in the treated A431 cancer cells increased in a time-dependent manner, and after 72 hours, it was 2.36 and 2.72 times compared to the control, respectively (p<0.001). The invasion activity of A431 cancer cells treated with the sublethal dose of Lacticin 3147 decreased in a dose-dependent manner. The lowest invasion activity was at concentration of 0.5 µM with minimal lethal effect (p<0.001).
The expression of the bax proapoptotic gene in A431 cells treated with IC50 concentration of Lacticin 3147 increased significantly (p<0.001). Also, the antiapoptotic bcl-2 gene expression reduction was significant in the same conditions. As a result, the use of Lacticin 3147 in the future may lead to a new strategy for the treatment of skin cancer.

Keywords

References

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medical journal of mashhad university of medical sciences
Volume 65, Issue 5
May and June 2022

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