The effect of High-Intensity Functional Training on oxidative stress and antioxidant markers in active men

Document Type : Research Paper

Authors

1 Master of Sports Psychology, La.C., Islamic Azad University, Lahijan, Iran.

2 Department of Physical Education and Sport Sciences Sha.C., Islamic Azad University, Shahrood, Iran (Corresponding Author)

Abstract

Introduction: Although high-intensity physical activities can lead to an increase in the production of free radicals and consequently elevate oxidative stress, regular exercise is capable of enhancing the body's antioxidant defense system and mitigating its adverse effects. The aim of this study was to investigate the effect of High-Intensity Functional Training (HIFT) on oxidative stress and antioxidant markers in active men. 
Methods: This semi-experimental study was conducted using a pretest-posttest design with experimental and control groups. The statistical population included physically active men who were members of sports clubs in Lahijan city. From the volunteers, 24 individuals (13 in the experimental group and 11 in the control group) were randomly assigned to the two groups based on inclusion and exclusion criteria. Blood samples were collected from the antecubital vein at baseline and after eight weeks of training to assess serum levels of MDA, SOD, GPX, and CAT. The HIFT program consisted of combined resistance and aerobic exercises performed three sessions per week for eight weeks. Data analysis was conducted using analysis of variance (ANOVA) via SPSS version 27.
Results: The findings revealed that after controlling for pretest scores, there were significant differences between the posttest scores of the two groups in SOD, CAT, GPX, and MDA levels, such that the experimental group showed significantly improved levels compared to the control group (P < 0.001).
Conclusion: Based on the findings of this study, HIFT improves oxidative status in active men by increasing antioxidant enzyme activity and reducing oxidative stress markers.

Keywords

Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 3
May and June 2025
Pages 691-702

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