A study on pseudo- positive and negative smears obtained from skin lesions suspected to Cutaneous Leishmaniasis at Emamreza Hospital and Health Centers of Mashhad.

Document Type : Research Paper

Authors

1 Faculty of Medicine, Mashhad University of Medical Sciences

2 استادیار گروه انگل شناسی و قارچ شناسی, دانشکده پزشکی, دانشگاه علوم پزشکی مشهد, ایران

3 Department of Parasitology and mycology, Faulty of Medicine, Mashhad University of Medical Sciences. Mashhad. Iran

4 Parasitology & Mycology Lab. Emam Reza Hospital, MUMS

5 دانشجو کارشناسی ارشد انگل شناسی

6 Professor of Parasitology and mycology, Emamreza Hospital, School of Medicine, Mashhad University of Medical Sciences.

Abstract

Introduction: Cutaneous Leishmaniasis (CL) is a parasitic skin disease, endemic worldwide. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. However sensitivity of direct smear is rather high, but it depends on the quality of sampling, staining and the expert of Laboratory technician and responsible Medical Parasitologist for the test. However the sensitivity of direct smear is not as high as molecular methods. The aim of this study is to identify pseudo-positive and pseudo negative direct smears obtained from skin lesions suspected to CL by PCR method.
Methods & Materials: Among direct smears obtained from patients with suspicious skin lesions to CL who referred to Emamreza Hospital and Health Centers of Mashhad, 100 positive Giemsa-stained smears and 50 negative Giemsa-stained smears were collected. DNA extraction performed by scraping the stained smears, obtained from Patients with skin lesions. To perform PCR.
Results: Among DNA extracted from negative smears obtained in Health Centers, 28 % showed positive result for Leishmnia tropica. There was only one pseudo-positive smear among the reported positive smears. Among smears obtained from Hospital Lab., only one pseudo-negative without any pseudo-positive smears was observed.
Conclusion: Direct microscopy on stained smears is a good and accurate with high sensitivity laboratory method for diagnosis of Leishmaniasis, if sampling and preparation of the smear is proper and an expert microscopist using a standard microscope reports the result.

Keywords

Main Subjects

References

  1. Kheirandish F, Sharafi AC, Kazemi B, Mohebali M, Sarlak A, Tarahi MJ, Holakouee K, Hajaran H. Identification of Leishmania species using PCR assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients. Iranian journal of parasitology. 2013 Jul;8(3):382.
  2. Fata A, Kiani B, Raouf Rahmati A, Tayefi M, Moradinejad F, Moghaddas E. Epidemiological Status of Cutaneous Leishmaniasis in Mashhad, Northeastern Iran, During 2015-2019 .Zahedan Journal of Research in Medical Sciences. 2021 Jan31;23(1).
  3. Fata A, Rezaei A, Moghadas E, Mousavi F, Shamsian AA., Identification of Cutaneous Leishmaniasis Species in Dargaz City, Iran.
  4. Mahmoudi MR, Mohajeri M, Tavakol Afshar J, Shakeri MT, Yazdanpanah MJ, Berenji F, Fata A.,  Molecular identification of Leishmania species causing cutaneous leishmaniasis in Mashhad, Iran.
  5. Salehi Gh,  Fata A, Ali MM, Mojtaba MB, Hushang R, Abdolghayom M. Molecular identification of Leishmania species in Taybad district, Iran. Asian Pacific Journal of Tropical Disease. 2014 Sep1;4:S9-535.
  6. Mohaghegh MA, Fata A, Salehi GH, Berenji F. Molecular identification of Leishmania species using samples obtained from negative stained smears. Iranian journal of parasitology. 2013 Apr;8(2):337.
  7. Naseri A, Fata A, Rezai A, Hedayatimoghadam M, Berengi F, Akbarzadeh O, Shamsian SA. Molecular identification of leishmania species in Torbat-e Heydarieh, Khorasan Razavi province, Iran. International Journal of Medical Research & Health Sciences. 2016;5(6):92-87.
  8. Shafiei R, Kalantari M, Yousefi M, Aspatwar A, Arzamani K, Bozorgomid A, Mirahmadi H, Soleimani A, Raeghi S. Bionomics and phylo-molecular Analysis of Leishmania species isolated from human lesions using ITS1 genes in north-east of Iran. Journal of Parasitic Diseases. 2021 Feb 10:1-8.
  9. Omidian M, Khosravi AD, Nazari M, Rashidi A. The comparison of histopathological findings and Poly merase chain reaction in lesion with primary clinical diagnosis of cutaneous leishmaniasis with negative smear. Pak J Med Sci 2008; Jan-Mar.24(1):96-9
  10. Gonzalez U, Pinart M, Reveiz L, Alvar J, Intervantion for old world Cutaneous leishmaniasis (Review), Published by John Wiley & Sons.2008;3-4
  11. Shirzad MR, Cutaneous Leishmaniasis Control Guideline in Iran. Zoonoses Control Department, Center for Communicable Disease Control, Ministry of health and medical education, Tehran, Iran; 2012.
  12. Elahi R, Fata A, Berenji F. Comparison of laboratory Methods in diagnosis of cutaneous leishmaniasis. Med J Mashhad Univ Med Sc. 1995; 38(1): 62-68.
  13. Fata A, Salehi SGh, Rafatpanah H, Musavi BM, Mohaghegh M, Movahedi A. Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran. Tropical Parasitology. 2015 Jan 1;5(1):50-4.
Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 6
January and February 2026
Pages 1646-1652

Files

Share

How to cite

Statistics