A Comparative Immunohisto chemical Diagnosis of Hodgkin’s lymphoma with Conventional Method

Document Type : Research Paper

Authors

1 professor of pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad,Iran

2 Assistant professor, Imam Reza Hospital,Mashhad University of Medical Sciences,Mashhad,Iran

3 Anatomical & Clinical pathologist

4 Statistical Consultant, Mashhad University of Medical Sciences,Mashhad,Iran

5 Medical Student,Mashhad University of Medical Sciences,Mashhad,Iran

Abstract

Introduction
Hodgkin’s lymphoma is a malignant neoplasm of B lymphocytes and less common T lymphocytes that morphologically can be mistaken with several benign and malignant diseases. These diseases have different course, treatments and prognosis from Hodgkin’s lymphoma. Immunohistochemical staining is very useful that will aid in separating Hodgkin’s Lymphoma from a series of benign and malignant lookalikes.
Materials and Methods
We Randomly selected 53 cases with primary diagnosis of Hodgkin’s lymphoma in pathology laboratory of Imam Reza Hospital from 1374-1384. to confirm or rule out of the primary diagnosis, microscopic evaluation and Immunohistochemical staining were done.
Results
Overall in 47 cases (88.7%), Hodgkin’s lymphoma was confirmed and 6 cases (11.3%) were non hodgkin lymphoma.A total 5 cases were T cell Rich large B cell lymphoma and 1 case was anaplasic large cell lymphoma probably. In Hodgkin’s lymphoma cases, the mean age was 40 years. Except for nodular sclerosis other types were more frequent in males. (M/F = 2.5).The commonest type was mixed cellularity (39 cases) (54.7%).
Conclusion
Misdiagnosis of Hodgkin’s lymphoma without Immunohistochemical staining was 11.3%. Because of significant difference between treatment and prognosis of Hodgkin’s lymphoma and other morphologically similar diseases, immunohistochemical staining particulary in lymphocytic depletion and nodular lymphocytic predominant Hodgkin’s lymphoma, is necessary and recommended.

Keywords

References

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medical journal of mashhad university of medical sciences
Volume 53, Issue 4 - Serial Number 4
November and December 2010
Pages 214-221

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