Study of CYP19a1 and 17beta_HSD gene expression in polycystic ovary syndrome in a mouse model (mus musculus)

Document Type : Research Paper

Authors

1 Master's Student, Department of Molecular Genetics, Faculty of Basic Sciences, University of Guilan, Rasht, Iran

2 Associate Professor, Department of Molecular Genetics, Faculty of Basic Sciences, University of Guilan, Rasht, Iran (Corresponding Author)

10.22038/mjms.2025.26168

Abstract

Introduction: Polycystic Ovary Syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age, characterized by hormonal imbalances, ovulatory dysfunction, and morphological changes in the ovaries. Understanding the molecular mechanisms of this disease, especially the pathways related to steroidogenesis, is crucial for improving diagnosis and developing targeted therapies.
Methods: This study aimed to investigate the expression patterns of two key steroidogenesis genes—CYP19A1 (encoding the aromatase enzyme) and 17β-HSD (17-beta-hydroxysteroid dehydrogenase)—in a mouse model of PCOS. Thirty-five female NMRI mice were divided into three groups: control (n=5), treated with testosterone enanthate (n=15), and treated with estradiol valerate (n=15). After 25 days of treatment, blood and ovarian samples were collected, and gene expression was assessed using Real-Time PCR. Histological analyses and quantification of ovarian structure were also performed.
Results: The results demonstrated a significant increase in CYP19A1 and 17β-HSD expression in the treated groups (P < 0.0001). While these findings indicate the involvement of these genes in the manifestations of the PCOS mouse model, clinical studies are necessary to validate them as non-invasive biomarkers in humans.

Keywords

Medical Journal of Mashhad university of Medical Sciences
Volume 68, Issue 1
March and April 2025

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