The effect of intense aerobic training on ICAM-1, VCAM-1 and RAGE expression in heart tissue in type 2 diabetes obese rats

Document Type : Research Paper

Authors

1 PhD Student in Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran.

2 Associate Professor, Department of Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran

3 Assistant Professor, Department of Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran

4 Assistant Professor, Department of Sports Physiology, Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran

Abstract

Introduction:The  expression of  adhesion  molecules  in  the  heart  tissue  is  considered  as  an important  indicator  in  the  estimation  of  coronary  artery  disease  risk.  Since  physical  activity  is  effective  in  reducing symptoms of cardiac disease, The purpose of this study was to The effect of intense aerobic training on gene expression ICAM-1, VCAM-1 and RAGE in heart tissue in type 2 diabetes obese rats.
Materials & Methods: During this experiment 14 diabetic rats with High-Dieting Diet Vistar race with an average weight of 220 ±20 grams, were randomly divided into two  groups, including the control group, and the  group intense aerobic.The training program of the intense aerobic training group for 6 weeks was 5 sessions per week in the form of running on a treadmill with 30 second repetitions and 2 minute active rest between each repetition. Blood sampling was performed 48 hours after the last training session. test data were analyzed by Independent  Samples t test.
Results:The results showed that intense aerobic exercise have significant effect on gene expression ICAM-1 and VCAM-1(P=0/001). However, it had  no significant effect on gene expression RAGE(P=0/34).
Conclusion: According to the results, in seems that Intense aerobic  trainings effectively reduce gene expressions ICAM-1 and VCAM-1 in left ventricular heart of diabetic rats and therefore can be considered  an  effective way  in reducing  pathogenesis cardiac signaling pathways in the heart of rats with type 2 diabetes.

Keywords


1.Yanai H, Adachi H, Masui Y, Katsuyama H, Kawaguchi A, Hakoshima M, et al. Exercise Therapy for
Patients With Type 2 Diabetes: A Narrative Review. J Clin Med Res 2018;10(5):365-69.
2.Thent ZC, Das S, LJ H. Role of exercise in the management of diabetes mellitus: the global scenario.
PLoS One 2013;8(11):1-8.
3.Lazar MA. How obesity causes diabetes: not atall tale. Science 2005; 307(5708):373-5.
4. Wu H, Sheng ZQ, Xie J, Li R, Chen L, Li GN. et al. Reduced HMGB 1-Mediated Pathway and Oxidative
Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in
DiabetesMellitus.Oxid Med Cell Longev 2016;983680:10.
5. Jia G, Hill MA, Sowers JR. Diabetic Cardiomyopathy:An Update of Mechanisms Contributing to This
Clinical Entity. Circ Res 2018; 122(4): 624-38.
6. Blake GJ. Novel clinical markers of vascular wall inflammation. Circ Res 2001;89(8):763-71.
7. Lim ST, Min SK, Park H, Park JH, Park JK. Effects of a healthy life exercise program on
arteriosclerosis adhesion molecules in elderly obese women. Journal of Physical Therapy Science
2015;27(5):1529–1532.
8. Wang RY, Yang YR, Yu SM. Protective effects of treadmill training on infarction in rats. Brain Res 2001;
922(1): 140-3.
9. Malik I, Danesh J, Whincup P, Bhatia V, Papacosta O, Walker M, et al. Soluble Adhesion Molecules and
Prediction of Coronary Heart Disease: A Prospective Study and Meta-Analysis. Lancet 2011; 35(6).
10. Ramasamy R, Schmidt AM. Receptor for Advanced Glycation End Products (RAGE) and Implications
for the Pathophysiology of Heart Failure. Curr Heart Fail Rep 2012; 9(2):107–116.
11. Basta G, Lazzerini G, Massaro M, Simoncini T, Tanganelli P, Fu C, et al. Advanced Glycation End
Products Activate Endothelium Through Signal-Transduction Receptor RAGE, AMechanism for
Amplification of Inflammatory Responses. American Heart Association, Inc 2016; 11:816- 822.
12. Patel S, Santani D. Role of NF-_B in the pathogenesis of diabetes and its associated complications.
Pharmacological Reports 2009; 61(4):595- 603.
13. Lorenzo O, Picatoste B, Ares-Carrasco S, Ramirez E, Egido J, Tunon J. Potential Role of Nuclear
Factor κB inDiabetic Cardiomyopathy. Hindawi Publishing Corporation Mediators of Inflammation 2011;
9(3):1-9.
14. Qin J, Goswami R, Dawson S, Dawson G. Expression of the receptor for advanced glycation end
products in oligodendrocytes in response to oxidative stress. J neuroscience Res 2008; 86(11): 2414-22.
15. Xie J, Mendez JD, Mendez-Valenzuela V. Aguilar-Hernández MM. Cellular signalling of the receptor
for advanced glycation end products (RAGE). Cellular signall 2013; 25(11): 2185-97.
16. Asad M, Sistani M, Barzegari A. The Effect of Eight Weeks of Continuous Endurance Training on
ICAM-1 and VCAM-1 Expression in the Heart Tissue of Rats. JBUMS 2019; 21 (1): 230-236. [Farsi].
17. Mogharnasi M, Gaeini A, Javadi E, Kordi M, Ravasi A.Sheikholeslami Vatani D. The Effect of
Endurance Training on Inflammatory Biomarkers and Lipid Profiles in Wistar Rats. Wjss 2009;2(2): 82-89.
[Farsi].
18. Mohammadi R, Matin Homaee H, Azerbaijani M A, Baesi K. effect of 12 week resistance training on
gene expressions rage, icam, vcam in the heart of diabetic rats with stz. Ijdld 2016;16 (1):1-8. [Farsi].
19. Taghibeigi Hoseinabadi H, Esfarjani F, Marandi M, Karami H. Effects of Eight Weeks of Aerobic
Training on Expression Levels of the HMGB1-RAGE/TLR4-NF-kB Proinflammatory Pathway in Cardiac
Tissue of Male Rats with Hyperglycemia. Iranian Journal of Endocrinology and Metabolism 2019;20 (5) :
246-252. [Farsi].
20. Khademi Y, Azarbayejani M, Hossini S. The impact of high intensity aerobic interval training (HIIT) and
flaxseed oil on ICAM-1 gene expression in heart tissue in male Wistar rats. Armaghane danesh 2016; 21
(9):873-886. [Farsi].
21. Mohammad shirazi R, Ghazalian F, Soori R, Abed Natanzi H, Gholami M . Comparison of the effect
of 10- weeks’ high-intensity and moderate-intensity aerobic training on ICAM-I and VCAM-I plasma
levels in obese child boys 13 to 15 years old. Scientific Journal of Rehabilitation Medicine 2020;8. [Farsi].
22. Bagheri Afsariehee M. The effect of Resistance training on ICAM-1, VCAM-1 and RAGE expression
in heart tissue in type 2 diabetes obese rats. PhD Thesis in Applied Sports Physiology, Islamic Azad
University, South Tehran Branch 2020. [Farsi].
23. Barzegari A, Asad M R, Ranjbar H. Effect of one Bout Continuous Versus Intermittent Aerobic
Exercise on Plasma Levels of Intercellular Adhesion Molecules 1 and Vascular Cell Adhesion Molecules 1
in Patients with Coronary Heart Disease. JSSU. 2020;27(11):2052-62. [Farsi].
24. Yazdanpazhooh S, Banaeifar A, Arshadi S, Eizadi M. The effect of resistance training on PPARy
expression in subcutaneous fat tissue of diabetic rats with high fat diet and STZ.Razi J Med Sci 2019;
26(8):68-77. [Farsi].
25. Kalhor H, Peeri M, Matin Homaee H, Izadi M. The Effect of 6 Weeks Resistance Training and HITT on
GLP-1 Gene Expression of Diabetic Rats. Iranian Journal of Diabetes and Obesity 2018; 10(1):42-9. [Farsi].
26. Hadipour Ahmadi S. The Synergystic Effect Of Aerobic Exercise And Probiotic Intake On Gene ICAM–
1 In Rats With Nonalcoholic Fatty Liver. Master Thesis in Sports Physiology, Islamic Azad University, East
Tehran Branch 2020. [Farsi].
27. Abednatanzi H, Choopani Z. The effect of six weeks of high intensity interval training (hiit) on plasmatic
levels of cellular adhesion molecules (icam-1) and lipid profile in young overweight women. Int Res J Appl
Basic Sci 2014;8(11): 2082- 8. [Farsi].
28. Kargarfard M, Lam ET, Shariat A, Asle Mohammadi M, Afrasiabi S, Shaw I, et al. Effects of endurance
and high intensity training on ICAM-1 and VCAM1 levels and arterial pressure in obese and normal weight
adolescents. Physician Sports medicine 2016; 44(3): 208-16. [Farsi].
29. Ding YH, Young CN, Luan X, Li J, Rafols JA, Clark JC, et al. Exercise preconditioning ameliorates
inflammatory injury in ischemicf rats during reperfusion. Acta Neuropathol (Berl) 2005; 109(3):237-46.
30. Koh Y. Park J. Cell adhesion molecules and exercise. J Inflamm Res 2018; 11: 297–306
31. Zoppini G, Targher G, Zamboni C, Venturi C, Cacciatori V, Moghetti P, et al . Effects of moderateintensity exercise training on plasma biomarders of inflammation and endothelial dysfunction in order
patients with type 2 diabetes. Nutrition, Metabolism & Cardiovascular Disease 2006;16: 543-549.
32. Haghir H, Hejazi SM, Minaee S. Changes of Serum Intercellular Adhesion Molecule-1, Vascular
Adhesion Molecule-1 and C-Reactive Protein in Middle-Aged Men with Heart Failure after Eight Weeks of
Aerobic Exercis. JSSU 2017;24 (12):1013-23. [Farsi].
33. Brevetti G, De Caterina M, Martone VD, Ungaro B, Corrado F, Silvestro A, et al. Exercise increases
soluble adhesion molecules ICAM-1 and VCAM-1 in patients with intermittent claudication.Clin Hemorheol
Microcirc 2001; 24(3):193-9.
34. Monchanin G, Serpero LD, Connes P, Tripette J, Woassi D, Bezin L, et al. Effects of progressive
andmaximal exercise on plasma levels of adhesion moleculesin athletes with sickle cell trait with or without
alphathalassemia. J Appl Physiol 2007;102(1):169-73.
35. Yun-Hong D, Chen NY, Xiaodong L, Jie Li, Jose A R, Justin CC. Exercise econditioning ameliorates
inflammatory injury in ischemicrats during reperfusion. Acta Neuropathol 2005;109(3):237-46.
36. Rankovic G, Milicic B, Savic T, Dindic B, Mancev Z. Effects of physicalexercise on inflammatory
parameters and risk for repeated acute coronary syndrome in patients with ischemic heart disease.
Vojnosanitetski pregled 2009;66(1):44- 48.
37. Hosseni Abrishami L, Hejazi SM, Rashid Lamir A, Khajeie R. The Effect of Eight Weeks of Continuous
and Intermittent Aerobic Exercise on Serum C-Reactive Protein and Adhesion Molecules in Men With Heart
Failure. Sabzevar University of Medical Sciences 2019;26(4). [Farsi].
38. Khademi Y, Ebadi H. The effect of HIIT training on the expression of VCAM-1 gene in heart tissue in
male Wistar rats, 2nd National Conference on Achievements in Sports Science and Health. Ahvaz 2018.
[Farsi].
39. Gu Q, Wang B, Zhang XF, Ma YP, Liu JD, Wang XZ. Contribution of receptor for advanced glycation
enproducts to vasculature-protecting effects of exercise training in aged rats. Eur J Pharmacol 2014;
741(27):186-94.
40. Zhang F, Su X, Huang G, Xin X-F, Cao E-H, Shi Y, et al. sRAGE alleviates neutrophilic asthma by
blocking HMGB1/RAGE signalling in airway dendritic cells. Sci Rep 2017; 7(1):14268.
41. Nowotny K, Jung T, HOhn A, Weber D, Grune T. Advanced Glycation End Products and Oxidative
Stress in Type 2 Diabetes Mellitus. Biomolecules 2016; 5(1):194-222.
42. Zhao J, Randive R, Stewart JA, Molecular mechanisms of AGE/RAGE-mediated fibrosis in the diabetic
heart. World Journal of Diabetes 2014; 5(6):860- 867.