ارتباط بین سطح سرمی و بزاقی هورمون کورتیزول

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار و فوق تخصص غدد،

2 استاد و فوق تخصص غدد،

3 استادیار آسیب شناسی

چکیده

مقدمه: اندازه گیری کورتیزول بزاق، علی رغم اطمینان و ارزان بودن هنوز به صورت گسترده استفاده نمی شود. مفید بودن آن در بررسی فعالیت محور هیپوتالاموس- هیپوفیز- آدرنال در موارد مختلف کلینیکی اثبات شده است. مراکز مختلفی هنوز در مقابل استفاده از این روش مقاومت می کنند. هدف از این مطالعه بررسی ارتباط سطح سرمی و بزاقی هورمون کورتیزول با روش رادیو ایمونواسی (RIA) است.
روش کار: این مطالعه توصیفی مقطعی از شهریور 1384 لغایت اسفند ماه 1384 بر 80 مراجعه کننده به آزمایشگاه بیمارستان قائم (عج) انجام شد. از 38 مرد و 42 زن با متوسط سن 5/16 ± 46 سال با در نظر گرفتن شرایط ورود به مطالعه نمونه سرمی و بزاقی گرفته شد. نمونه های سرمی و بزاقی بین ساعت 8 تا 9 صبح جمع آوری شد. کورتیزول بزاق و کورتیزول تام سرمی به روش رادیوایمونواسی (RIA) اندازه گیری شد.
نتایج: میانگین کورتیزول سرم 35/8 ± 72/19 میکروگروم در دسی لیتر و میانگین کورتیزول بزاق 07/0 ± 18/0 میکروگرم در دسی لیتر محاسبه شد. همبستگی معنی داری بین میانگین کورتیزول سرم و بزاق وجود دارد (003/0=p ، 35/0=r).
نتیجه گیری: نتایج این مطالعه نشان می دهد که اندازه گیری کورتیزول بزاق با روش رادیوایمونواسی (RIA) می تواند جایگزین اندازه گیری تام سرمی این هورمون شود. چرا که اندازه گیری بزاقی این هورمون نشانه قسمت آزاد و از نظر بیولوژیک فعال این هورمون است و نیز روش ساده غیر تهاجمی و بدون استرس می باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Level of Salivary and Serum Cortisol

نویسندگان [English]

  • M Haratian 1
  • R Rajabian 2
  • H Ayatollahi 3
چکیده [English]

I





ntroduction:In spite of its convenience and inexpensiveness, measurement of salivary cortisol has not gained widespread popularity. Indeed, even though its usefulness in the assessment of activity of hypothalamic-pituitary-adrenal (HPA) axis has been reported in various clinical conditions, several centers are still resistant to adopting this procedure. The aim of this study is to evaluate the correlation between salivary and serum cortisol by radioimmunoassay (RIA).
Materials and Methods: This descriptive crass-sectional study carried out in September to February 2005 on 80 patients at laboratory of Ghaem Hospital of Mashhad University of Medical Sciences. A total of 42 women and 38 men aged 46 16.5 were studied. Paired saliva and serum samples were obtained after specialized protocols. Salivary and blood samples were collected at 8 to 9 A.M. Cortisol in saliva and total cortisol in serum were measured with a commercial radioimmunoassay (RIA).
Results: Mean serum cortisol was 19.72 8.35 and mean salivary cortisol was 0.18 0.07 µg/dL. Significant linear correlation was found between salivary and serum total cortisol (r=0/35, p<0.001).
Conclusion: Results indicate that salivary cortisol measures with radioimmunoassay (RIA) can be used in place of serum total cortisol, based on the evidence that salivary level represents the biologicaly active and free fraction of this hormone, and is non invasive and unstressful.

کلیدواژه‌ها [English]

  • Serum
  • Salivary
  • Cortisol
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