اثر تمرینات تناوبی با شدت بالا (HIIT) بر پپتید وابسته به ژن کلسی‌تونین در عضله نعلی موش های صحرایی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، گروه تربیت‌بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران

2 استادیار فیزیولوژی ورزش ، گروه تربیت بدنی ،واحد بروجرد ، دانشگاه آزاد اسلامی ، بروجرد ، ایران.(نویسنده مسئول)

3 استادیار فیزیولوژی ورزش، گروه تربیت‌بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران.

4 دانشیار فیزیولوژی ورزش، گروه تربیت بدنی، دانشگاه رازی، کرمانشاه، ایران

چکیده

زمینه و هدف: اتصال عصبی عضلانی و انتقال سیناپسی به انواع عضلات اسکلتی ممکن است تحت تأثیر تمرینات بدنی قرار گیرند. هدف از انجام این پژوهش بررسی اثر یک دوره تمرینات اینتروال با شدت بالا (HIIT) بر بیان پپتید وابسته به ژن کلسی‌تونین (CGRP) در عضله نعلی موش های صحرایی نر است.
روش تحقیق: در این مطالعه تجربی، 12 سر موش صحرایی نر نژاد ویستار با وزن 220-200 گرم، در دو گروه تمرین و کنترل، به صورت تصادفی قرار گرفتند. تمرین HIIT شامل 6 تا 12 تکرار تمرین تناوبی 2 دقیقه‌ای بود که بین هر تکرار یک دقیقه استراحت وجود داشت. تمرین برای 5 روز در هفته و 6 هفته طراحی شده بود. شرایط گروه کنترل مشابه گروه تمرین بود، ولی تمرین ورزشی انجام نمی‌دادند. 48 ساعت پس از آخرین جلسه تمرینی، موش‌های صحرایی توسط روش استاندارد بیهوش، قربانی و بافت عضله نعلی تمامی موش‌ها استخراج شد، سپس برای تعیین میزان بیان CGRP از روش وسترن‌بلات استفاده شد. از آزمون آماری تی مستقل برای بررسی اختلاف معناداری میانگین‌ بین گروه‌های کنترل و (HIIT)استفاده شد. سطح معنی‌داری 05/0>p در نظر گرفته شد.
یافته‌ها: یافته‌های آماری برای مقادیر بیان پروتئین گیرنده CGRP نشان داد که اجرای 6 هفته پروتکل تمرینی HIIT باعث افزایش در بیان پروتئینCGRP در مقایسه با گروه کنترل شد؛ که این مقدار از نظر آماری معنی‌دار نبود (078/0=p).

کلیدواژه‌ها


عنوان مقاله [English]

The effect of high-intensity interval training on calcitonin gene-dependent peptide in horseshoe muscles of male Wistar rats

نویسندگان [English]

  • Firoze Khamverdi 1
  • Ahmad Hematfar 2
  • Mania Roozbayani 3
  • Naser Behpour 4
1 PhD student in exercise physiology, Department of Physical Education, Borujard Branch, Islamic Azad University, Borujard, Iran
2 Assistant Professor of Exercise Physiology, Department of Physical Education, Borujard Branch, Islamic Azad University, Borujard, Iran. (Responsible author)
3 Assistant Professor of Exercise Physiology, Department of Physical Education, Borujard Branch, Islamic Azad University, Borujard, Iran.
4 Associate Professor of Exercise Physiology, Department of Physical Education, Razi University, Kermanshah, Iran
چکیده [English]

Background and Aim: Neuromuscular junction and synaptic transmission to skeletal muscle types may be affected by physical exercise. The aim of this study was to investigate the effect of a high-intensity interval training (HIIT) on CGRP gene expression in the horseshoe muscles of male Wistar rats.
Materials and Methods: In this experimental study, 12 male Wistar rats weighing 200-220 g, with a sleep cycle of 12.12, free access to food and water and an ambient temperature of 25 ° C, were randomly divided into two training and control groups. HIIT training consisted of 6 to 12 repetitions of a 2-minute periodic exercise with a one-minute break between each repetition. The workout was designed for 5 days a week and 6 weeks. The conditions of the control group were similar to the training group but they did not exercise. Finally, 48 hours after the last training session, rats were extracted by standard anesthesia, victim and horseshoe muscle method of all mice, and then Western blot method was used to determine the expression of CGRP protein. Significance level was considered p> 0.05.
Results: Statistical findings from the output of independent t-test for CGRP receptor protein expression values showed that implementation of six weeks of HIIT training protocol caused an increase in CGRP protein expression compared to the control group, which was not statistically significant (p = 0.078).

کلیدواژه‌ها [English]

  • Calcitonin gene-related peptide (CGRP)
  • high-intensity interval training (HIIT)
  • skeletal muscle
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