سنتز نانونیوزوم‌های حاوی عصاره گیاه درمنه بیابانی (L. Artemisia deserti): شناسایی ترکیبات گیاهی، اثرات ضدمیکروبی و سمیت سلولی نانونیوزوم‌های سنتز شده

نوع مقاله : مقاله پژوهشی

نویسنده

گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران

چکیده

مقدمه
نانونیوزوم‌ها، وزیکول‌های چندلایه‌ای هستند که امروزه به عنوان یکی از سیستم‌های نوین انتقال هدفمند دارو، مورد استفاده قرار می‌گیرند. در این مطالعه، عصاره گیاه درمنه بیابانی (Artemisia deserti L.) در نانونیوزوم انکپسوله شد، اثرات ضدمیکروبی و سمیت سلولی آن مورد مطالعه قرار گرفت.
روش کار
در این مطالعه تجربی، ابندا نانونیوزوم‌های حاوی عصاره گیاه درمنه دشتی سنتز گردید، خصوصیات ظاهری و ساختاری آن بررسی شد. پس از تایید ساختار، اثرات ضدمیکروبی و ضدبیوفیلمی آن بر روی سویه‌های بیماری‌زای کلبسیلا پنومونیه بررسی شد. در انتها، به منظور بررسی سازگاری زیستی، از روش رنگ سنجی سمیت سلولی نانونیوزوم‌ها بر روی رده سلولی فیبروبلاست نرمال HFF استفاده شد.
نتایج
نتایج سنتز نیوزوم ها نشان داد که نانونیوزوم های سنتز شده دارای ساختار کروی بوده و دارای اندازه 3/29 ± 4/125 نانومتر می باشند. علاوه بر آن، نتایج FTIR سنتز نیوزوم‌ها را تایید نمود. نتایج اثرات ضدمیکروبی نشان داد که نانونیوزوم های حاوی عصاره درمنه دارای اثرات ضدمیکروبی و ضدبیوفیلمی معنادارتری نسبت به عصاره تنها دارند، بطوریکه حداقل غلظت مهاری (MIC) نیوزوم‌ها بین 2 تا 4 برابر کاهش یافته بود. هم چنین، نتایج سمیت سلولی نشان داد که نیوزوم‌های سنتز شده دارای اثرات سمیت سلولی معناداری نیستند.
نتیجه‌گیری
بطور کلی، نتایج این مطالعه نشان داد که نانونیوزوم‌ها میتوانند به عنوان یک سیستم دارورسانی مناسب برای عصاره گیاه درمنه عمل کنند، بطوریکه میزان اثرات ضدمیکروبی و ضدبیوفیلمی را افزایش دهند. بنابراین با مطالعات بیشتر میتوان از این سیستم به عنوان سیستم بهینه دارورسانی جهت اهداف دارویی درمانی استفاده نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Synthesis of nanoniosomes loaded Artemisia deserti extract: identification of chemical compounds, antimicrobial effects and cytotoxicity of nanoniosomes

نویسنده [English]

  • Amir Mirzaie
Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
چکیده [English]

Introduction
Nanoniosomes are multi-layered vesicles that are used as one of the new targeted drug delivery systems today. In this study, the extract of the Artemisia deserti L. was encapsulated in nanonisomes and its antimicrobial and cytotoxic effects were studied.
Materials and Methods
In this experimental study, nanonisomes containing the extract of Artemisia deserti were synthesized and their appearance and structural characteristics were investigated. After confirming the structure, its antimicrobial and anti-biofilm effects on Klebsiella pneumoniae pathogenic strains were investigated. Finally, in order to check the biocompatibility, the colorimetric method of nanonisomes cytotoxicity was used on HFF normal fibroblast cell line.
Results
The results of niosomes synthesis showed that the synthesized nanoniosomes have a spherical structure and have a size of 125.4 ± 29.3 nm. In addition, FTIR results confirmed the synthesis of niosomes. The results of the antimicrobial effects showed that the nanoniosomes containing the medicinal extract have more significant antimicrobial and anti-biofilm effects than the extract alone, so that the minimum inhibitory concentration (MIC) of the niosomes was reduced between 2 and 4 times. Also, the cytotoxicity results showed that the synthesized niosomes do not have significant cytotoxicity effects.
Conclusion
In general, the results of this study showed that nanonisomes can act as a suitable drug delivery system for medicinal plant extract to increase the amount of antimicrobial and anti-biofilm effects. Therefore, with further studies, this system can be used as an optimal drug delivery system for medicinal purposes.

کلیدواژه‌ها [English]

  • Nanoniosome
  • Artemisia deserti
  • Klebsiella pneumoniae
  • antimicrobial effects
  • anti-biofilm

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 67، شماره 3
مرداد-شهریور1403
مرداد و شهریور 1403
صفحه 803-822

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