تاثیر تمرینات هوازی بر مقاومت انسولین و محور AMPK/PI3K در عضله نعلی رت های دیابتی نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی واحد بروجرد، بروجرد، ایران

2 گروه فیزیولوژی ورزشی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران.

3 گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی واحد بروجرد، بروجرد، ایران

چکیده

مقدمه: نقش مولفه های ژنتیکی در هایپرتروفی و نیمرخ گلیسیمیک بویژه در حضور دیابت نوع 2 به خوبی مشخص شده است. این مطالعه با هدف تعیین اثر تمرینات هوازی بر محور AMPK/PI3K در عضله نعلی و مقاومت انسولین در رت های دیابتی نوع انجام گرفت.
روش کار: برای این منظور، 14 سر رت نر ویستار 10 هفته ای(10±220 گرم) توسط تزریق درون صفاقی نیکوتین آمید و STZ دیابتی نوع 2 شده و به شیوه تصادفی به 2 گروه کنترل(7=n) و ورزش(7=n) تقسیم شدند. رت های گروه ورزش در یک دوره تمرینات 10 هفته ای هوازی به تعداد 5 جلسه در هفته در قالب دویدن روی تریدمیل شرکت نمودند. 48 ساعت پس از آخرین جلسه تمرین، بیانPI3K و AMPK در عضله نعلی همچین سطوح گلوکز ناشتا در گروه های مورد مطالعه اندازه گیری شد. از مقادیر ناشتایی گلوکز و انسولین جهت محاسبه مقاومت انسولین استفاده شد. داده ها توسط آزمون تی مستقل بین گروه ها مقایسه شدند.
نتایج: در مقایسه با گروه کنترل، تمرینات هوازی به افزایش معنی دار بیانPI3K در عضله نعلی(005/0 =P) و کاهش معنی دار گلوکز ناشتا(001/0 =P) و مقاومت انسولین(010/0 =P) منجر شد. بیان AMPK در پاسخ به تمرینات هوازی نسبت به گروه کنترل تغییر نکرد(238/0 =P).
نتیجه گیری: بر پایه این یافته ها، بهبود گلوکز و مقاومت انسولین در پاسخ به تمرینات هوازی را شاید بتوان به افزایش بیان PI3K در عضله نعلی نسبت داد. شناخت مکانیسم های عهده دار هایپرتروفی عضلانی در پاسخ به ورزش نیازمند مطالعات سلولی مولکولی بیشتر در این زمینه است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of aerobic training on insulin resistance and AMPK/PI3K axis in Soleus muscle of type 2 diabetic rats

نویسندگان [English]

  • Hamid Eslami 1
  • Mojtaba Eizadi 2
  • Mania Rozbayani 3
1 Ph.D student, Department of Exercise physiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2 Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran.
3 Department of Exercise physiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
چکیده [English]

Introduction: The role of genetic components in hypertrophy and glycemic profile especially in the presence of type 2 diabetes is well defined. This study aimed to determine the effect of aerobic training on AMPK/PI3K axis in Soleus muscle and insulin resistance in type 2 diabetic rats.
Methods: For this purpose, type 2 diabetic induced in fourteen male Wistar rats aged 10 week (220 ± 10 g) by intraperitoneal injection of nicotinamide and STZ and were randomly selected in exercise (n = 7) and control (n = 7) groups. The exercise rats participated in an aerobic training program (5 times weekly) in the form of running on the treadmill. 48 hours after the lasting exercise session, PI3K and AMPK expression in soleus muscle as well as fasting glucose were measured. Fasting glucose and insulin values were used to calculate insulin resistance. Data compared by independent t test between groups.
Results: Compared to control group, aerobic training resulted in significant increase in PI3K expression in soleus muscle (P = 0.005) and significant decrease in fasting glucose (P = 0.001) and insulin resistance (P = 0.010). AMPK expression did not change in response to aerobic training compare with control group (P = 0.238).
Conclusion: Based on these data, improved glucose and insulin resistance in response to aerobic training may be attributed to increased PI3K expression in soleus muscle. Understanding the mechanisms responsible for hypertrophy in response to exercise requires more studies in this area.

کلیدواژه‌ها [English]

  • Type 2 diabetes
  • Gene expression Aerobic training
  • Glycemic profile
  • Hypertrophy

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 68، شماره 1
فروردین-اردیبهشت1404
فروردین و اردیبهشت 1404
صفحه 32-40

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